pSilencer™ 2.1-U6 puro Vector Map
pSilencer™ 2.1-U6 puro Annotated Promoter/Cloning Site Region
We recommend the following primers for sequencing:
T7 primer: 5'-TAATACGACTCACTATAGGG-3'
2.0rev primer: 5'-AGGCGATTAAGTTGGGTA-3'
pSilencer™ 2.1-U6 puro Restriction Sites (Vector Size: 4455 bp)
Enzymes with single Restriction Sites
| Site | Enzyme |
|---|---|
| 3586 | AccI |
| 1250 | AflIII |
| 3416 | AgeI |
| 2143 | AhdI |
| 1666 | AlwNI |
| 3365 | AvrII |
| 461 | BanHI |
| 3387 | BmtI |
| 4204 | BsaBI |
| 3533 | BsiWI |
| 4293 | BsmI |
| 3390 | BspMI |
| 3611 | BstEII |
| 794 | EcoRI |
| 399 | HindIII |
| 4305 | HpaI |
| 4292 | MfeI |
| 3852 | MscI |
| 3383 | NjeI |
| 1250 | PciI |
| 3061 | PflMI |
| 759 | PmlI |
| 4325 | PsiI |
| 3401 | PstI |
| 3593 | RsrII |
| 3691 | SacII |
| 3585 | SalI |
| 1134 | SapI |
| 3401 | SbfI |
| 2623 | ScaI |
| 3318 | SfiI |
| 2947 | SspI |
| 3519 | Tth111I |
| 4193 | XbaI |
| 2742 | XmnI |
Non-Cutting Enzymes:
| Enzyme |
|---|
| AarI |
| Acc65I |
| AfeI |
| AflII |
| AleI |
| AloI |
| AloI' |
| ApaI |
| AscI |
| AsiSI |
| BaeI |
| BaeI' |
| BbsI |
| BbvCI |
| BclI |
| BglII |
| BlpI |
| BmgBI |
| Bpu10I |
| BsgI |
| BspEI |
| BsrGI |
| BstBI |
| BstXI |
| BstZ17I |
| Bsu36I |
| BtrI |
| ClaI |
| EcoICRI |
| EcoNI |
| EcoRV |
| FalI |
| FalI' |
| FseI |
| FspAI |
| KpnI |
| MluI |
| NaeI |
| NgoMIV |
| NotI |
| NruI |
| PacI |
| PmeI |
| PpuMI |
| PshAI |
| PspOMI |
| PsrI |
| PsrI' |
| SacI |
| SanDI |
| SgfI |
| SgrAI |
| SnaBI |
| SpeI |
| SrfI |
| SwaI |
| TaqII' |
| XcmI |
| XhoI |
