pSilencer™ 3.1-H1 neo Vector Map

pSilencer™ 3.1-H1 neo Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.


pSilencer™ 3.1-H1 neo Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'

3.0rev: 5'-GAGTTAGCTCACTCATTAGGC-3'





pSilencer™ 3.1-H1 neo Restriction Sites (Vector Size: 4285 bp)

Enzymes with single Restriction Sites

Site Enzyme
4220 AatII
915 AflIII
1808 AhdI
1331 AlwNI
3772 AvaI
3793 AvrII
495 BamHI
3226 BanII
2313 BcgI
2347 BcgI`
3742 BclI
3283 BsaAI
3728 BsaBI
790 BsaXI
760 BsaXI`
3803 BseRI
45 BsmBI
2667 EagI
4274 EcoO109I
396 EcoRI
556 HindIII
3505 MscI
184 NdeI
2903 NruI
915 PciI
3538 PstI
3068 RsrII
2288 ScaI
4025 SexAI
3774 SmaI
3798 StuI
3468 Tth111I
3772 XmaI
2407 XmnI
4218 ZraI

Non-Cutting Enzymes:

Enzyme
AarI
Acc65I
AccI
AfeI
AflII
AgeI
AleI
AloI
AloI'
ApaI
AscI
AsiSI
BaeI
BaeI`
BbsI
BbvCI
BglII
BlpI
BmgBI
BmtI
Bpu10I
BsgI
BsiWI
BspEI
BsrGI
BstBI
BstEII
BstXI
BstZ17I
Bsu36I
BtrI
ClaI
DraIII
EcoICRI
EcoNI
EcoRV
FseI
FspAI
HincII
HpaI
KpnI
MfeI
MluI
NheI
NotI
PacI
PflMI
PmeI
PmlI
PpuMI
PshAI
PspOMI
PsrI
PsrI`
SacI
SacII
SalI
SanDI
SbfI
SfiI
SgfI
SgrAI
SnaBI
SpeI
SrfI
SwaI
TaqII'
XbaI
XcmI
XhoI