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By including all of the components from direct sample conversion to purification, the Cells-to-CpG™ System provides a validated, flexible, simplified bisulfite conversion solution that is ideal for real-time PCR, CE, and next generation sequencing workflows. Choose a 50-reaction format or 2x96-plate format for more flexibility in performing your bisulfite conversion. Validated controls, including the Cells-to-CpG™ Bisulfite Conversion and Quantitation Control Kit as well as the Cells-to-CpG™ Methylated and Unmethylated Control Kit, are available to use through bisulfite conversion and amplification of the bisulfite-converted DNA.
The Cells-To-CpG™ Bisulfite Conversion Kit is designed to provide quantitatively reliable results verified with available controls. DNA recovery after conversion correlates linearly to the input amount with no bias observed between non-methylated and methylated samples (Figure 3).
Starting with cells, tissues, blood, FFPE samples, or purified DNA, directly lyse and denature sample for subsequent bisulfite conversion, desalting, and desulfonation. The protocol takes less than 3 hours, which is a significant reduction in time compared to other available methods that require at least 18 hours.
Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.