blood droplet
Use of liquid biopsy dPCR assays for monitoring resistance and response

Digital PCR (dPCR) is an established method for the fast and reliable identification of significant cancer-related mutations. For liquid biopsy analysis, dPCR assays are a precise, cost-effective, and rapid method for monitoring response and resistance to treatment by testing for relevant cancer-driver and therapy-resistant mutations.

The TaqMan dPCR Liquid Biopsy assays detect and quantify the most common cancer-related mutations (e.g., EGFR, BRAF, KRAS, PIK3CA, JAK2), as well as therapy-resistant mutations (e.g., EGFR T790M). They have been verified and are guaranteed* to perform on the QuantStudio 3D Digital PCR System and other dPCR and droplet digital PCR (ddPCR) instruments, including the Bio-Rad QX-100™ and QX-200™ systems.**

Poster: TaqMan dPCR Liquid Biopsy Assays targeting the TERT promoter region

The TERT promotor region has a high incidence of mutations in multiple cancer types, yet detection of these mutations is difficult due to the high GC content and repetitive elements in this region. In this poster, learn about how we successfully designed and optimized TaqMan dPCR Liquid Biopsy Assays for TERT C228T and TERT C250T mutations with detection to 0.1%.

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Key features of the TaqMan Liquid Biopsy dPCR Assays

  • Optimized dPCR performance—wet lab–verified TaqMan assays targeting over 100 common cancer mutations including NSCLC, breast cancer, and CRC markers including:
  • o EGFR p.T790M
  • o EGFR p.L858R
  • o BRAF p.V600E
  • o KRAS p.G12D
  • o TERT p.C228T
  • o TERT p.C250T
  • Highly sensitive—detect and quantify rare mutant prevalence to ≤0.1%
  • Cost-effective, single-tube assay—single-tube format includes both wild type and mutant alleles
  • Streamlined analysis—enhanced bioinformatics tools for better quantification of rare mutations
  • Guaranteed performance—backed by the TaqMan performance guarantee

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Additional liquid biopsy solutions

Targeted next-generation sequencing (NGS) assays

Exosome isolation

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** Bio-Rad and Bio-Rad QX100 and QX200 Droplet Digital PCR Systems are trademarks of Bio-Rad Laboratories, Inc., which is not affiliated with Thermo Fisher Scientific.  These statements have not been reviewed or endorsed by Bio-Rad. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under license.