The Applied Biosystems HIV-1 Genotyping Kit harnesses gold-standard Sanger sequencing technology to amplify and reliably sequence the diverse and rapidly evolving HIV-1 virus. To address the HIV surveillance needs of the global community, this kit offers broad genotyping coverage of HIV-1 Group M subtypes from plasma and dried blood spot (DBS) samples to detect resistance to protease inhibitors, nucleoside reverse-transcriptase inhibitors, and non-nucleoside reverse-transcriptase inhibitors.

This improved Applied Biosystems assay workflow was developed by Thermo Fisher Scientific in collaboration with the US Centers for Disease Control and Prevention.

Coming soon: The Applied Biosystems HIV-1 Genotyping Kit with Integrase

The new HIV-1 Genotyping Kit will cover the protease, reverse transcriptase, and integrase regions of the pol gene. Be the first to know when it becomes available. 

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The Applied Biosystems HIV-1 Genotyping Kit features:

  • A standardized, cost-effective assay in a scalable workflow
  • A total workflow of about 14 hours for processing 14 samples from extracted viral RNA to results, with total hands-on time of less than 3 hours
  • The ability to detect mutations in the protease and reverse transcriptase regions of the HIV-1 pol gene in subtypes A, B, C, D, AE, CRF01_AE, CRF02_AG, and AG
  • Easy-to-read sequencing results with free software for assembly and interpretation

 

Assay workflow

proflex

After sample processing and RNA extraction, samples undergo an initial reverse transcription + PCR, followed by a second nested PCR step for mutation targets of interest. The HIV-1 Genotyping Kit Amplification Module includes the RT-PCR master mix, the nested PCR master mix, and necessary positive and negative controls that allow for a simple, streamlined workflow as you move into your cleanup and sequencing steps.

3500xl

Once you confirm and clean up your targeted PCR product, the HIV-1 Genotyping Kit Sequencing Module is used for cycle sequencing. Included in this kit are the forward/reverse pre-formulated sequencing mixes for the targets of interest and the pGEM sequencing control. 

Free access to Exatype™ software is provided with the purchase of HIV-1 Genotyping Kits.  This easy-to-use application allows for:

  • Data upload directly to a secure web interface
  • Analyzing sequencing data files, creating consensus for target regions and reporting drug resistance into a user-friendly report
  • Integration with laboratory information management systems (LIMS)
  • Multiple users under a single organization license
proflex

After sample processing and RNA extraction, samples undergo an initial reverse transcription + PCR, followed by a second nested PCR step for mutation targets of interest. The HIV-1 Genotyping Kit Amplification Module includes the RT-PCR master mix, the nested PCR master mix, and necessary positive and negative controls that allow for a simple, streamlined workflow as you move into your cleanup and sequencing steps.

3500xl

Once you confirm and clean up your targeted PCR product, the HIV-1 Genotyping Kit Sequencing Module is used for cycle sequencing. Included in this kit are the forward/reverse pre-formulated sequencing mixes for the targets of interest and the pGEM sequencing control. 

Free access to Exatype™ software is provided with the purchase of HIV-1 Genotyping Kits.  This easy-to-use application allows for:

  • Data upload directly to a secure web interface
  • Analyzing sequencing data files, creating consensus for target regions and reporting drug resistance into a user-friendly report
  • Integration with laboratory information management systems (LIMS)
  • Multiple users under a single organization license
genotyping-hiv-detect-drug-resistance


Ordering information

HIV-1-Genotyping-Kit-Amplification-Module-

HIV-1 Genotyping Kit Amplification Module

The Amplification module contains sufficient reagents for 48 RT-PCR reactions and 48 nested PCR reactions.

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HIV-1-Genotyping-Kit-Cycle-Sequencing-Module-

HIV-1 Genotyping Kit Cycle Sequencing Module

The Cycle Sequencing module contains sufficient reagents for 48 sets of bidirectional cycle sequencing reactions.

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cdc webinar

Having an accurate and sensitive test for HIV-1 genetic variants that resist common antiretroviral therapeutics is critical for epidemiologic and genetic surveillance studies. In this webinar, Dr. Joy Chang of the United States Centers for Disease Control (CDC) discusses the CDC’s experience investigating the Applied Biosystems HIV-1 Genotyping Kit with Integrase.

Watch presentation ›

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