We’ve collected these protocols, publications, and scientific posters citing the use of Gibco™ KnockOut™ products for stem cell culture. A keyword search is available for the items listed in the table below. Alternatively, the columns may be sorted by resource type, resource name, application, or the products used. Our Stem Cell Research Learning Center is home to many more learning resources to help you maximize your stem cell research efforts and outcomes.

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Type Name Application Products used
Protocol Creation of embryoid bodies from iPSCs using complete KnockOut Serum Replacement feeder-free medium EB formation in human PSCs KnockOut Serum Replacement; KnockOut DMEM/F-12
Protocol Culturing human embryonic stem cells (hESCs) on MEF-conditioned medium Conditioned medium culture of human ESCs KnockOut Serum Replacement; Geltrex hESC-Qualified Matrix; CELLstart CTS substrate
Protocol Culturing induced pluripotent stem cells (iPSCs) on MEF-conditioned medium Conditioned medium culture of human iPSCs KnockOut Serum Replacement
Protocol Derivation of dopaminergic neurons (from human embryonic stem cells) Differentiation of human ESCs KnockOut Serum Replacement; Geltrex hESC-Qualified Matrix; CELLstart CTS substrate
Protocol Feeder-dependent culture of human embryonic stem cells (hESCs) Feeder-dependent culture of human ESCs KnockOut Serum Replacement
Protocol Feeder-dependent culture of human induced pluripotent stem cells (hiPSCs) Feeder-dependent culture of human iPSCs KnockOut Serum Replacement
Protocol Feeder-dependent culture and passaging mouse embryonic stem cells (mESCs) in KnockOut Serum Replacement with leukemia inhibitory factor (LIF) Feeder-dependent culture of mouse ESCs KnockOut Serum Replacement; KnockOut DMEM
Protocol Feeder-dependent culture of human pluripotent stem cells (hPSCs) with KnockOut™ Serum Replacement – Multi-Species Feeder-dependent culture of hPSCs KnockOut™ Serum Replacement – Multi-Species; DMEM/F-12
Protocol Feeder-dependent culture of mouse pluripotent stem cells (mPSCs) with Leukemia Inhibitory Factor (LIF) Feeder-dependent culture of mouse PSCs KnockOut™ Serum Replacement – Multi-Species; KnockOut™ DMEM
Protocol Formation of embryoid bodies (EBs) from mouse embryonic stem cells (mESCs) EB formation in mouse ESCs KnockOut Serum Replacement; KnockOut DMEM
Protocol Formation of Embryoid Bodies (EBs) from Mouse Pluripotent Stem Cells (mPSCs) EB formation from mouse PSCs KnockOut™ Serum Replacement – Multi-Species; KnockOut™ DMEM
Publication (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture Culture of human ESCs; comparison of FBS and KnockOut SR KnockOut Serum Replacement; KnockOut DMEM
Publication (2001) Feeder-free growth of undifferentiated human embryonic stem cells Feeder-free culture of human hESCs KnockOut Serum Replacement; KnockOut DMEM
Publication (2003) Generating CNS neurons from embryonic, fetal, and adult stem cells Differentiation of human SCs KnockOut DMEM
Publication (2004) Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor Culture of human and mouse ESCs KnockOut Serum Replacement; KnockOut DMEM
Publication (2004) Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor Culture of human ESCS; comparison of FBS and KnockOut SR KnockOut Serum Replacement; KnockOut DMEM
Publication (2004) Feeder layer- and serum-free culture of human embryonic stem cells Feeder-dependent culture of hESCs KnockOut Serum Replacement; KnockOut DMEM
Publication (2004) Electromechanical integration of cardiomyocytes derived from human embryonic stem cells Integration of human ESCs KnockOut DMEM
Publication (2004) Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media Serum-free culture KnockOut Serum Replacement; KnockOut DMEM
Publication (2005) Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells Feeder-dependent culture of hESCs; comparison of FBS and KnockOut SR KnockOut Serum Replacement
Publication (2006) An efficient method for the derivation of mouse embryonic stem cells Derivation of mouse ESCs; comparison of FBS and KnockOut SR KnockOut Serum Replacement; KnockOut DMEM
Publication (2006) Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES-7 Differentiation of human ESCs; comparison of FBS and KnockOut SR KnockOut Serum Replacement; KnockOut DMEM
Publication (2006) Unique gene expression signature by human embryonic stem cells cultured under serum-free conditions correlates with their enhanced and prolonged growth in an undifferentiated stage Serum-free culture; comparison of FBS and KnockOut SR KnockOut Serum Replacement; KnockOut DMEM
Publication (2006) Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines Serum-free culture; comparison of FBS and KnockOut SR KnockOut Serum Replacement
Publication (2007) New cell lines from mouse epiblast share defining features with human embryonic stem cells Derivation of mouse ESCs KnockOut Serum Replacement; KnockOut DMEM
Publication (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors Generation of human iPSCs KnockOut Serum Replacement
Publication (2007) Generation of induced pluripotent stem cells in the absence of drug selection Generation of mouse iPSCs; comparison of FBS and KnockOut SR KnockOut Serum Replacement
Publication (2008) Disease-specific induced pluripotent stem cells Generation of human iPSCs KnockOut Serum Replacement
Publication (2008) Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 Generation of human iPSCs KnockOut Serum Replacement; KnockOut DMEM
Publication (2008) Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts Generation of Rhesus monkey IPSCs KnockOut Serum Replacement
Publication (2008) Reprogramming of human somatic cells to pluripotency with defined factors Reprogramming of human somatic cells KnockOut DMEM
Publication (2008) Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds Reprogramming of mouse fibroblasts KnockOut Serum Replacement; KnockOut DMEM
Publication (2009) Derivation of induced pluripotent stem cells from pig somatic cells Generation of pig iPSCs KnockOut Serum Replacement; KnockOut DMEM
Publication (2010) Characterization of human Huntington's Disease cell model from induced pluripotent stem cells Characterization of human iPSCs KnockOut Serum Replacement; KnockOut DMEM/F-12
Publication (2010) Human dermal stem cells differentiate into functional epidermal melanocytes Differentiation of human SCs KnockOut Serum Replacement; KnockOut DMEM/F-12
Publication (2010) Generation of novel rat and human pluripotent stem cells by reprogramming and chemical approaches Generation of rat and human PSCs KnockOut Serum Replacement; KnockOut DMEM
Publication (2010) Efficient and rapid generation of induced pluripotent stem cells using an alternative culture medium Generation of mouse iPSCs; comparison of FBS and KnockOut SR KnockOut Serum Replacement
Publication (2011) Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells Differentiation of mouse ESCs KnockOut DMEM/F-12
Publication (2011) Induced pluripotent stem cells for neural tissue engineering Generation of human iPSCs KnockOut DMEM/F-12
Publication (2011) Generation of germline-competent rat induced pluripotent stem cells. Generation of rat iPSCs KnockOut Serum Replacement; KnockOut DMEM
Publication (2011) Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors Reprogramming of fibroblasts; comparison of FBS and KnockOut SR KnockOut Serum Replacement; KnockOut DMEM; KnockOut DMEM/F-12
Publication (2012) Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non obese diabetic mouse Derivation of mouse ESCs; comparison of FBS and KnockOut SR KnockOut Serum Replacement; KnockOut DMEM
Publication (2012) Signaling defects in iPSC-derived fragile X premutation neurons Generation of human iPSCs KnockOut Serum Replacement; KnockOut DMEM/F-12
Publication (2012) Efficient iPS cell production with the MyoD transactivation domain in serum-free culture Serum-free culture; comparison of FBS and KnockOut SR KnockOut Serum Replacement
Publication (2013) Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons Differentiation of hESC, hiPSC and iPSCs KnockOut Serum Replacement
Publication (2016) Serum-based culture conditions provoke gene expression variability in mouse embryonic stem cells as revealed by single-cell analysis Generation of mouse ESCs; comparison of FBS and KnockOut SR KnockOut Serum Replacement
Scientific poster Genome editing of α-Synuclein in iPSCs from a donor with multiple system atrophy Genome editing of iPSCs grown on MEFs KnockOut Serum Replacement; KnockOut DMEM
Scientific poster CytoTune-iPS 2.0 Sendai Reprogramming Kit for high efficiency reprogramming of fibroblasts and blood-derived cells Reprogramming of fibroblasts KnockOut Serum Replacement; CytoTune 2.0 Reprogramming Kit
Scientific poster Enabling successful reprogramming of peripheral blood mononuclear cells to induced pluripotent stem cells with the CytoTune-iPS 2.0 Sendai Reprogramming Kit Reprogramming of PBMCs KnockOut Serum Replacement; CytoTune 2.0 Reprogramming Kit
Scientific poster (ISSCR 2011) Synthetic surfaces for the culture of human embryonic and adult stem cells Matrices for growing hESCs and iPSCs KnockOut Serum Replacement; Geltrex hESC-Qualified Matrix; CELLstart CTS
Scientific poster (ISSCR 2011) High-quality episomal human iPSC derived from cord blood with a zero rootprint: applications in serum-free and xeno-free culture Serum-free culture KnockOut Serum Replacement; Gibco Episomal hiPSCs
Scientific poster (ISSCR 2011) Xeno-free Culture Systems for Stem Cells Xeno-free culture of H9 hESCs KnockOut DMEM; KnockOut SR XenoFree CTS; KnockOut SR GF Cocktail CTS
Scientific poster (ISSCR 2011) Generation of zero-footprint iPSCs in xeno-free or feeder-free with CytoTune™ -iPS Sendai Reprogramming Kit Xeno-free generation of iPSCs KnockOut DMEM; KnockOut SR XenoFree CTS; KnockOut SR GF Cocktail CTS; CytoTune-iPS Sendai Reprogramming Kit
Scientific poster (ISSCR 2012) Xeno-free culture systems for pluripotent stem cells Generation of iPSCs KnockOut DMEM CTS; KnockOut SR XenoFree CTS; KnockOut SR GF Cocktail CTS