Invitrogen

IL-13 Monoclonal Antibody (eBio13A), Functional Grade, eBioscience™

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IL-13 Antibody in Immunohistochemistry (IHC) — Figure 4 HpARI Suppresses Responses to Alternaria Allergen (A) Day 17 BAL eosinophil numbers after Alternaria allergen, OVA protein, and HpARI administration on day 0 (sensitization), and OVA protein alone on days 14, 15, and 16 (challenge). (B) Lung ILC2 IL-5 production from mice in (A). (C) Lung ILC2 IL-13 production from mice in (A). (D) Lung resistance in methacholine challenge from mice treated as in (A). (E) Lung compliance in methacholine challenge from mice treated as in (A). (F) H&E- (top panels) and PAS-stained (bottom panels) lung sections from mice treated as in (A). Scale bars indicate 100 mum. (G) H&E scoring of sections from mice treated as in (A). (H) PAS scoring of sections from mice treated as in (A). Alternaria model data representative of 2-3 repeat experiments, each with 4-6 mice per group. (I) Mice were subcutaneously infected with N. brasiliensis , and HpARI administered intranasally on days 0, 1, and 2 of infection. Lung larvae were counted 3 days after infection. (J) Day 6 intestinal N. brasiliensis worms from mice treated as in (I). (K) Day 3 and day 6 BAL eosinophil numbers from mice treated as in (I). Error bars show SEM.

IL-13 Antibody in Flow Cytometry (Flow) — Fig. 2 Arf1 ablation induces the infiltration and activation of immune cells in Lgr5/Apc mice. a , b Immunofluorescent staining of GFP and CD8a ( a ) or CD4 ( b ) in the intestine of the indicated mice. c - e Flow cytometric analysis of gut APCs: DCs ( c ), inflammatory DCs ( d ), and macrophages ( e ) ( n = 3 mice each group; ** p < 0.01, t -test; repeat two independent experiments). f - m Flow cytometric analysis of the immune cells of LPLs: CD4 + T cells ( f ), IFNgamma + CD4 + T cells ( g ), CD8 + T cells ( h ), IFNgamma + CD8 + T cells ( i ), gammadelta T cells ( j ), NK cells ( k ), Treg cells ( l ), and IL-13 + CD4 + T cells, ( m ) ( n = 3 or 5). n - p Flow cytometric analysis of the immune cells of IELs: CD4 + T cells ( n ), CD8alphabeta + T cells ( o ), and CD8alphaalpha + T cells ( p ) ( n = 5 mice per group; * p < 0.05, ** p < 0.01, t- test; repeat two independent experiments). q - u Relative gene expression of the indicated chemokines, cytokines, and granzymes ( n = 5 mice each group, repeat two independent experiments). v Immunofluorescent staining of GFP and pZap70 in the intestine of the indicated mice ( n = 5 mice each group; * p < 0.05, ** p < 0.01, *** p < 0.001, t -test; repeat two independent experiments). Each experiment replicate two times. Data are shown as the mean +- SEM. Scale bars are as indicated.

IL-13 Antibody in Neutralization (Neu) — Figure 6 The number of M2 AMs is decreased in Tlr2 -/- mice after S. aureus priming. ( a ) ELISA of IL-13 in BALF from WT mice and Tlr2 -/- mice at day 3 after S. aureus priming. ( b ) Quantitative RT-PCR analysis of the expression of Arg1, Fizz1, Ym1 and IL-10 mRNAs in AMs from control WT mice and anti-IL-13 neutralizing antibody-treated WT mice at day 3 after S. aureus priming. ( c ) Hematoxylin and eosin staining of the lungs from control mice, S. aureus -primed mice and S. aureus -primed anti-IL-13 neutralizing antibody-treated mice at day 5 after infecting with 0.5 HA of PR8. Scale bars represent 200 mum. ( d ) Survival of control mice, S. aureus -primed mice and S. aureus -primed anti-IL-13 neutralizing antibody-treated mice after infecting with 0.5 HA of PR8. ( e ) The number of AMs in BALF from WT mice and Tlr2 -/- mice at day 3 after S. aureus priming. ( f ) CD11b expression on AMs from WT mice and Tlr2 -/- mice at day 3 after S. aureus priming. ( g ) Quantitative RT-PCR analysis of the expression of Arg1, Fizz1, Ym1, IL-10 and TGF-beta mRNA in AMs from WT mice and Tlr2 -/- mice at day 3 after S. aureus priming. Two-tailed Student's t -tests; NS, not significant; * P <0.05, ** P <0.01, *** P <0.001. Data are expressed as mean+-s.e.m. Data represent three independent experiments with at least five mice per group in ( a , b , e - g ), or represent two independent experiments with at least five mice per group in ( c , d ).

Product Details

16-7133-85

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 15 publications 15 publications
ELISA (ELISA)	1-4 µg/mL	-
Neutralization (Neu)	-	View 1 publication 1 publication
Functional Assay (FN)	Assay-Dependent	-
Control (Ctrl)	Assay-Dependent	-

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBio13A
Conjugate	Functional Grade Alexa Fluor 488 APC-eFluor 780 Brilliant UV 737 Brilliant UV 805 eFluor 450 eFluor 660 PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	4° C
RRID	AB_763558

Product Specific Information

Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Applications Reported: The eBio13A antibody has been reported useful for ELISA and ELISPOT capture, as well as intracellular staining for flow cytometric analysis.

Applications Tested: The Functional Grade Purified eBio13A antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA and ELISPOT capture.

The eBio13A antibody has been tested as the capture antibody in a sandwich ELISA (and ELISPOT) for analysis of mouse IL-13, in combination with the biotinylated eBio1316H antibody for detection (13-7135) and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1.0 - 4.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8.0 pg/mL should be included in each ELISA plate.

The Functional Grade Purified eBio1316H antibody (16-7135) is recommended for in vitro blocking studies.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice.

Nature communications

Wang G,Xu J,Zhao J,Yin W,Liu D,Chen W,Hou SX

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

Ovalbumin induces natural killer cells to secrete Th2 cytokines IL‑5 and IL‑13 in a mouse model of asthma.

Molecular medicine reports

Chen Z,Wang L

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes.

Nature communications

Galle-Treger L,Sankaranarayanan I,Hurrell BP,Howard E,Lo R,Maazi H,Lewis G,Banie H,Epstein AL,Hu P,Rehan VK,Gilliland FD,Allayee H,Soroosh P,Sharpe AH,Akbari O

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Tue Feb 12 00:00:00 EST 2019

Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation.

Genome medicine

Pramanik J,Chen X,Kar G,Henriksson J,Gomes T,Park JE,Natarajan K,Meyer KB,Miao Z,McKenzie ANJ,Mahata B,Teichmann SA

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Wed Oct 24 00:00:00 EDT 2018

Autoimmune Th17 Cells Induced Synovial Stromal and Innate Lymphoid Cell Secretion of the Cytokine GM-CSF to Initiate and Augment Autoimmune Arthritis.

Immunity

Hirota K,Hashimoto M,Ito Y,Matsuura M,Ito H,Tanaka M,Watanabe H,Kondoh G,Tanaka A,Yasuda K,Kopf M,Potocnik AJ,Stockinger B,Sakaguchi N,Sakaguchi S

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

Tissue-Restricted Adaptive Type 2 Immunity Is Orchestrated by Expression of the Costimulatory Molecule OX40L on Group 2 Innate Lymphoid Cells.

Immunity

Halim TYF,Rana BMJ,Walker JA,Kerscher B,Knolle MD,Jolin HE,Serrao EM,Haim-Vilmovsky L,Teichmann SA,Rodewald HR,Botto M,Vyse TJ,Fallon PG,Li Z,Withers DR,McKenzie ANJ

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells.

Arthritis research & therapy

Saigusa R,Asano Y,Taniguchi T,Hirabayashi M,Nakamura K,Miura S,Yamashita T,Takahashi T,Ichimura Y,Toyama T,Yoshizaki A,Trojanowska M,Sato S

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Wed Feb 07 00:00:00 EST 2018

HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33.

Immunity

Osbourn M,Soares DC,Vacca F,Cohen ES,Scott IC,Gregory WF,Smyth DJ,Toivakka M,Kemter AM,le Bihan T,Wear M,Hoving D,Filbey KJ,Hewitson JP,Henderson H,Gonzàlez-Cìscar A,Errington C,Vermeren S,Astier AL,Wallace WA,Schwarze J,Ivens AC,Maizels RM,McSorley HJ

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Immunohistochemistry

Tue Oct 17 00:00:00 EDT 2017

HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33.

Immunity

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Immunohistochemistry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Epidermal Growth Factor Receptor Expression Licenses Type-2 Helper T Cells to Function in a T Cell Receptor-Independent Fashion.

Immunity

Minutti CM,Drube S,Blair N,Schwartz C,McCrae JC,McKenzie AN,Kamradt T,Mokry M,Coffer PJ,Sibilia M,Sijts AJ,Fallon PG,Maizels RM,Zaiss DM

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region.

PloS one

Kim K,Kim N,Lee GR

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Thu Jul 07 00:00:00 EDT 2016

RSV vaccine-enhanced disease is orchestrated by the combined actions of distinct CD4 T cell subsets.

PLoS pathogens

Knudson CJ,Hartwig SM,Meyerholz DK,Varga SM

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2015

Cutting edge: maresin-1 engages regulatory T cells to limit type 2 innate lymphoid cell activation and promote resolution of lung inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Krishnamoorthy N,Burkett PR,Dalli J,Abdulnour RE,Colas R,Ramon S,Phipps RP,Petasis NA,Kuchroo VK,Serhan CN,Levy BD

16-7133 was used in Flow cytometry/Cell sorting to reveal that lung MaR1 augments de novo Treg generation, and that both Tregs and MaR1 inhibit ILC2s to restrain allergic lung inflammation.

Sun Feb 01 00:00:00 EST 2015

Bacterial colonization dampens influenza-mediated acute lung injury via induction of M2 alveolar macrophages.

Nature communications

Wang J,Li F,Sun R,Gao X,Wei H,Li LJ,Tian Z

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Neutralization

Mon Dec 30 00:00:00 EST 2013

Type 2 innate lymphoid cells control eosinophil homeostasis.

Nature

Nussbaum JC,Van Dyken SJ,von Moltke J,Cheng LE,Mohapatra A,Molofsky AB,Thornton EE,Krummel MF,Chawla A,Liang HE,Locksley RM

Published figure using IL-13 monoclonal antibody (Product # 16-7133-85) in Flow Cytometry

Thu Oct 10 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

Gene Aliases: Il-13; Il13

UniProt ID: (Mouse) P20109

Entrez Gene ID: (Mouse) 16163

Function(s)