Invitrogen

IL-13 Monoclonal Antibody (eBio13A), PerCP-eFluor™ 710, eBioscience™

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IL-13 Antibody in Flow Cytometry (Flow) — BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82), soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82), Mouse IL-2 Recombinant Protein (Product # 14-8021-64), and Mouse IL-4 Recombinant Protein (Product # 14-8041-80). Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) for 5 hours. Following restimulation, cells were fixed and permeabilized and then stained with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Rat IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4301-80) (left) or 0.125 µg of Anti-Mouse IL-13 PerCP-eFluor® 710 (right). Viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

Product Details

46-7133-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.25 µg/test	View 11 publications 11 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), PerCP-eFluor™ 710, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBio13A
Conjugate	PerCP-eFluor™ 710 Alexa Fluor 488 APC-eFluor 780 Brilliant UV 737 Brilliant UV 805 eFluor 450 eFluor 660 Functional Grade PE PE-Cyanine7 PE-eFluor 610 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_11218496

Product Specific Information

Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Applications Reported: This eBio13A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio13A antibody has been tested by intracellular staining and flow cytometric analysis of Th2-polarized mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice.

Nature communications

Wang G,Xu J,Zhao J,Yin W,Liu D,Chen W,Hou SX

Published figure using IL-13 monoclonal antibody (Product # 46-7133-82) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes.

Nature communications

Galle-Treger L,Sankaranarayanan I,Hurrell BP,Howard E,Lo R,Maazi H,Lewis G,Banie H,Epstein AL,Hu P,Rehan VK,Gilliland FD,Allayee H,Soroosh P,Sharpe AH,Akbari O

Published figure using IL-13 monoclonal antibody (Product # 46-7133-82) in Flow Cytometry

Tue Feb 12 00:00:00 EST 2019

Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation.

Genome medicine

Pramanik J,Chen X,Kar G,Henriksson J,Gomes T,Park JE,Natarajan K,Meyer KB,Miao Z,McKenzie ANJ,Mahata B,Teichmann SA

Published figure using IL-13 monoclonal antibody (Product # 46-7133-82) in Flow Cytometry

Wed Oct 24 00:00:00 EDT 2018

Autoimmune Th17 Cells Induced Synovial Stromal and Innate Lymphoid Cell Secretion of the Cytokine GM-CSF to Initiate and Augment Autoimmune Arthritis.

Immunity

Hirota K,Hashimoto M,Ito Y,Matsuura M,Ito H,Tanaka M,Watanabe H,Kondoh G,Tanaka A,Yasuda K,Kopf M,Potocnik AJ,Stockinger B,Sakaguchi N,Sakaguchi S

Published figure using IL-13 monoclonal antibody (Product # 46-7133-82) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

46-7133 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 EST 2018

Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells.

Arthritis research & therapy

Saigusa R,Asano Y,Taniguchi T,Hirabayashi M,Nakamura K,Miura S,Yamashita T,Takahashi T,Ichimura Y,Toyama T,Yoshizaki A,Trojanowska M,Sato S

Published figure using IL-13 monoclonal antibody (Product # 46-7133-82) in Flow Cytometry

Wed Feb 07 00:00:00 EST 2018

HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33.

Immunity

Osbourn M,Soares DC,Vacca F,Cohen ES,Scott IC,Gregory WF,Smyth DJ,Toivakka M,Kemter AM,le Bihan T,Wear M,Hoving D,Filbey KJ,Hewitson JP,Henderson H,Gonzàlez-Cìscar A,Errington C,Vermeren S,Astier AL,Wallace WA,Schwarze J,Ivens AC,Maizels RM,McSorley HJ

Published figure using IL-13 monoclonal antibody (Product # 46-7133-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using IL-13 monoclonal antibody (Product # 46-7133-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Epidermal Growth Factor Receptor Expression Licenses Type-2 Helper T Cells to Function in a T Cell Receptor-Independent Fashion.

Immunity

Minutti CM,Drube S,Blair N,Schwartz C,McCrae JC,McKenzie AN,Kamradt T,Mokry M,Coffer PJ,Sibilia M,Sijts AJ,Fallon PG,Maizels RM,Zaiss DM

Published figure using IL-13 monoclonal antibody (Product # 46-7133-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region.

PloS one

Kim K,Kim N,Lee GR

Published figure using IL-13 monoclonal antibody (Product # 46-7133-82) in Flow Cytometry

Thu Jul 07 00:00:00 EDT 2016

Type 2 innate lymphoid cells control eosinophil homeostasis.

Nature

Nussbaum JC,Van Dyken SJ,von Moltke J,Cheng LE,Mohapatra A,Molofsky AB,Thornton EE,Krummel MF,Chawla A,Liang HE,Locksley RM

Published figure using IL-13 monoclonal antibody (Product # 46-7133-82) in Flow Cytometry

Thu Oct 10 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

Gene Aliases: Il-13; Il13

UniProt ID: (Mouse) P20109

Entrez Gene ID: (Mouse) 16163

Function(s)