COL34013-EXP-RUSH-GTS-NPI-Creative-Concept-TaqMan-COVID-19-Mutation-Panel-Creative-Hero

Choose from a menu of 22 verified real-time PCR assays to build your own custom Applied Biosystems TaqMan SARS-CoV-2 Mutation Panel and get the answers you need today, while preparing you for what’s to come. This scalable solution lets you run a few or hundreds of samples to identify one or many mutations—all on your current real-time PCR instrumentation.

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Features of the TaqMan SARS-CoV-2 Mutation Panel include:

  • Customizable—build your own custom panel from a menu of 22 verified real-time PCR assays that allows you to identify currently relevant SARS-CoV-2 mutations and adapt quickly as additional mutations and variants emerge
  • Convenient—use your current real-time PCR instrumentation to conduct follow-up testing of SARS-CoV-2 samples
  • Scalable—run a few or hundreds of samples to identify for one or many mutations
  • Unique, streamlined workflow—combines our gold-standard TaqMan SNP Genotyping Assays with a 1-step RT-PCR reaction; go from RNA to results in just over 1 hour


Product details

Targets Choose from a menu of 22 verified real-time PCR SNP assays to build your custom panel
Assay design

Sequence-specific forward and reverse primers to amplify the target sequence region. The reverse primer also primes reverse transcription of the SARS-CoV-2 genomic RNA sequences. Each assay includes two TaqMan minor groove binder (MGB) probes with nonfluorescent quenchers (NFQ):

  • One VIC dye labeled probe to detect the reference sequence
  • One FAM dye labeled probe to detect the mutation sequence
  • Available sizes 300 reactions 
    1,000 reactions 
    Sample input RNA extracted from SARS-CoV-2 samples with a CT value of less or equal to 30 
    Turn around time 1 hour and 10 min from extracted RNA to results
    Recommended instruments Any real-time PCR instrument such as the Applied Biosystems 7500, 7500 Fast, QuantStudio 5, and QuantStudio 7 systems 
    Recommended software QuantStudio Design and Analysis Software v2.5 or later with the Genotyping Analysis Module


    Workflow

    Performance

    Our menu of verified real-time PCR assays provide robust detection of SARS-CoV-2 mutations. Below are results (cluster plots) as viewed using QuantStudio Design and Analysis Software 2.5 with the Genotyping Analysis Module. These cluster plots show very clear discrimination between the wild type samples (red dots along the x-axis) and the mutation samples (blue dots along the y-axis).

    Training Support

    In this short, 2 video tutorial, you will learn how to:

    • Set up the instrument software for detecting the SARS CoV-2 mutation panel
    • Analyze the data with the Applied Biosystems™ Design and Analysis Software 2.5 or later with the Genotyping Analysis Module using the run files from all Applied Biosystems real-time PCR instruments

    Go to thermofisher.com/educationconnect and log into your account or create a new one! 

    Analysis software

    Use QuantStudio Design and Analysis Software v2.5 or later with the Genotyping Analysis Module.

    Ordering information

    Mutation Gene Associated Variants Assay ID Catalog # 4332077 300 rxn Catalog # 4332075 1000 rxn
    delH69V70 S B.1.1.7, B.1.258, B.1.525  AN9HXTM
    N501Y S P.1, B.1.1.7, B.1.351 ANPRYZA
    E484K S P.1, B.1.1.33, B.1.351, B.1.525 ANU7GMZ
    K417N S B.1.351 ANZTTXP
    K417T S P.1 AN49ARF
    N439K S B.1.258 ANPRYXU
    D614G S B.1.1.207, P.1, B.1.1.33, B.1.1.7, B.1.177, B.1.258, B.1.351, B.1.525, Mink Variant ANDKDET
    A222V S B.1.177 ANT2M3N
    Y453F S Mink Variant ANWDA9H
    P681H S B.1.1.207, B.1.1.7 ANCFHV6
    Q27stop ORF8 B.1.1.7 ANH6NNF
    delY144 S B.1.1.7 ANAAN6D
    A570D S B.1.1.7 ANU7GR9
    T716I S B.1.1.7 ANXG4WR
    S982A S B.1.1.7 AN49AMT
    A1708D ORF1 B.1.1.7 ANAAN7M
    A701V S B.1.351 AN49ATD
    L242_244L S B.1.351 ANFVZJM
    D80A S B.1.351 ANCFHWG
    L18F S P.1, B.1.351 ANXG4TV
    R246I S B.1.351 ANYMYDT
    D215G S B.1.351 ANT2M6T


    Additional products needed

    Master mix


    Recommended controls

    Product  
    GeneArt DNA string or plasmid controls  thermofisher.com/geneart​