Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
ELISA Sample Preparation Protocols |
Learn detailed step-by-step instructions for preparing your samples for an ELISA experiment. Sample preparation protocols are available for various common sample types, including adherent and suspension cells, cell culture supernatant, cell lysate, blood (plasma, serum) and rat tissues (liver, lung, blood). The protocols provided below are general guidelines and may need further optimization to meet the specific requirements of your experimental plan. If you need additional help, our technical support team is ready to assist you. For ready-to-use ELISA kits, please refer to the specific protocol included with the kit.
Learn sample preparation of adherent and suspension cells in a 96-well format.
Learn the protocols to extract cytoplasmic and nuclear fractions from cultured cells.
Learn the protocols to separate plasma and serum from blood.
Learn sample preparation of rat liver, and lung, and protocol for rat blood collection.
Learn best practices for common sample types to run a successful ELISA assay.
Learn tips to best compare ELISA kits from different vendors.
Enhance your ELISA assay performance and navigate through common pitfalls.
Explore ELISA kit formats, including complete, ready-to-use kits and preoptimized reagents for designing your own assay.
Find HRP and other enzyme substrates for ELISA applications in a range of sensitivities for different methods including colorimetric, chemiluminescent, and fluorescent detection.
Learn more about centrifuges, cold storage equipment, dispensers, incubator/shakers, microplates, pipettes and tips, and microplate readers and washers to help meet all your ELISA needs.
Select from a wide variety of easy-to-use and reliable blocking buffers and reagents for ELISA applications.
Explore our immunoassays for different research areas, including immuno-oncology, cell signaling, neurobiology, inflammation and cytokine storm, hallmarks of cancer, CAR T cell therapy and more.
For Research Use Only. Not for use in diagnostic procedures.