Introduction

Learn detailed step-by-step instructions for preparing your samples for an ELISA experiment. Sample preparation protocols are available for various common sample types, including adherent and suspension cells, cell culture supernatant, cell lysate, blood (plasma, serum) and rat tissues (liver, lung, blood). The protocols provided below are general guidelines and may need further optimization to meet the specific requirements of your experimental plan. If you need additional help, our technical support team is ready to assist you. For ready-to-use ELISA kits, please refer to the specific protocol included with the kit.


Educational guides and technical note


Additional ELISA products

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ELISA Kits and Components

Explore ELISA kit formats, including complete, ready-to-use kits and preoptimized reagents for designing your own assay.

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ELISA Enzyme Substrates

Find HRP and other enzyme substrates for ELISA applications in a range of sensitivities for different methods including colorimetric, chemiluminescent, and fluorescent detection.

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ELISA Instrumentation and Equipment

Learn more about centrifuges, cold storage equipment, dispensers, incubator/shakers, microplates, pipettes and tips, and microplate readers and washers to help meet all your ELISA needs.

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ELISA Buffers and Reagents

Select from a wide variety of easy-to-use and reliable blocking buffers and reagents for ELISA applications.

Immunoassay Research Areas

Explore our immunoassays for different research areas, including immuno-oncology, cell signaling, neurobiology, inflammation and cytokine storm, hallmarks of cancer, CAR T cell therapy and more.

Learn more about Immunoassay Research Areas

Microplate readers

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For Research Use Only. Not for use in diagnostic procedures.

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