SuperScript® III Platinum® One-Step qRT-PCR Kit

Catalog number: 11732020

Invitrogen™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

The SuperScript® III Platinum® One-Step qRT-PCR Kits combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. The SuperScript® III Platinum® One-Step qRT-PCR Kit provides sensitive, specific detection with fluorogenic primers and probes, while the SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR Kit offers the ease and convenience of SYBR® Green I detection

SuperScript® III Platinum® One-Step qRT-PCR Kits provide:

• SuperScript® III RT for higher temperature cDNA synthesis for greater success with difficult RNA secondary structure
• Platinum® Taq DNA Polymerase with hot-start technology for improved specificity
• LUX™ Fluorogenic Primers (Figure 1) or dual-labeled fluorogenic probes (Figure 2) for superior detection performance
• SYBR® Green I dye for easy and convenient detection (Figure 3)
• Validated performance on multiple real-time instrument platforms, including rotor-based systems
For Research Use Only. Not for use in diagnostic procedures.

Specifications

PCR Method: 1-Step qRT-PCR
Detection Method: Primer-Probe Detection
Reaction Speed: Standard
For Use With (Equipment): Cepheid SmartCycler, BioRad iCycler iQ, BioRad MyiQ, MJ Opticon, Stratagene Mx3005P, Stratagene Mx4000, Stratagene Mx3000P, MJ Chromo4, BioRad iQ5, 7500 System
Sample Type (General): RNA
Product Size: 100 reactions
Shipping Condition: Dry Ice

Contents & storage

• 100 µl SuperScript® III/Platinum® Taq Mix
• 2 × 1.25 ml 2X Reaction Mix
• 1 ml Magnesium Sulfate (50 mM)
• 100 µl ROX Reference Dye (25 µM)

Store components at -20°C. ROX Reference Dye must be stored in the dark.

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Manuals & protocols

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