Staining of 24-hour LPS-stimulated normal human peripheral blood cells with 0.25 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (open histogram) or 0.25 µg of Anti-CCL2 (MCP-1) PE (filled histogram). Cells in the monocyte gate were used for analysis.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Product Details

12-7096-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H5

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Ambient (domestic); Wet ice (international)

AB_466171

Product Specific Information

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.

Applications Reported:The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and neutralization. (Please use Functional Grade purified 2H5 (Product # 16-7096-81) in functional assays).

Applications Tested: This 2H5 antibody is tested by flow cytometric analysis of fixed permabilized LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells. Has been tested by flow cytometric analysis of fixed permabilized LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ccl2; chemokine (C-C motif) ligand 2; chemotactic and activating factor precursor; Genbank Staff Annotation: The small inducible cytokine A2 (SWISS-PROT ACC P13500) is encoded in this mRNA, although it is interrupted and partial, perhaps indicating that this CDS is not the true product of the gamma.1 gene.; H-MCP-1; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; JE; MGC9434; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte chemotactic protein; monocyte chemotactic protein 1; monocyte secretory protein JE; Mutations: 171:OK; platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; small-inducible cytokine A2; unnamed protein product

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Gene Aliases: GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF

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UniProt ID: (Human) P13500, (Mouse) P10148, (Rat) P14844

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Entrez Gene ID: (Human) 6347, (Mouse) 20296, (Rat) 24770

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Function(s)

protein kinase activity receptor binding protein binding chemokine activity CCR2 chemokine receptor binding chemoattractant activity chemokine receptor binding CCR chemokine receptor binding G-protein coupled receptor binding cytokine activity heparin binding

Process(es)

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CCL2 (MCP-1) Monoclonal Antibody (2H5), PE, eBioscience™

CCL2 (MCP-1) Antibody (12-7096-82) in Flow

Product Details

12-7096-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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