SulfoLink™ Coupling Resin
SulfoLink™ Coupling Resin
Thermo Scientific™

SulfoLink™ Coupling Resin

Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalentRead more
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Catalog NumberQuantity
2040250 mL
2040110 mL
20404250 mL
Catalog number 20402
Price (USD)/ 50 mL
778.65
Online exclusive
796.00 
Save 17.35 (2%)
-
Add to cart
Quantity:
50 mL
Request bulk or custom format
Price (USD)/ 50 mL
778.65
Online exclusive
796.00 
Save 17.35 (2%)
Add to cart
SulfoLink™ Coupling Resin
Catalog number20402
Price (USD)/ 50 mL
778.65
Online exclusive
796.00 
Save 17.35 (2%)
-
Add to cart
Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.

Features of SulfoLink Coupling Resin:

Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
Fast—spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)
Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
High capacity—Immobilize 1 to 2 mg of peptide or 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin

When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest. Choose one of two convenient SulfoLink Immobilization Kits for peptides or proteins. Each kit contains all the reagents for preparing the sample and five columns containing 2 mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately.

Applications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

Related Products
SulfoLink™ Immobilization Kit for Peptides, 2 mL
SulfoLink™ Immobilization Kit for Proteins, 2 mL
SulfoLink™ Immobilization Trial Kit, 2 mL
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormLiquid Suspension
Product LineSulfoLink™
Quantity50 mL
TypeResin
Stationary PhaseHaloacetyl
Sufficient For1 mg Peptide or 10 mg Antibody per mL of Resin
FormatBottle
Column TypeAffinity, Agarose Resin
Unit Size50 mL
Contents & Storage
Upon receipt store product in the dark at 4°C. Product is shipped at ambient temperature.