SIA (succinimidyl iodoacetate)

Catalog number: 22349

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

Features of SIA:

Reactive groups: NHS ester and iodoacetyl
Reactive toward: amino and sulfhydryl groups
• Shortest sulfhydryl-reactive and amine-reactive heterobifunctional crosslinker available; compare to SIAB
• NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond
• Iodoacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bond
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)

Product References:

Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.


Spacer: Short
Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Product Size: 50 mg
Labeling Method: Chemical Labeling
Crosslinker Type: Heterobifunctional

Contents & storage

Upon receipt store at 4°C protected from moisture. Product is shipped at ambient temperature.


Manuals & protocols