Pierce™ Controlled Protein-Protein Crosslinking Kit
Pierce™ Controlled Protein-Protein Crosslinking Kit
Thermo Scientific™

Pierce™ Controlled Protein-Protein Crosslinking Kit

The Thermo Scientific Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes andRead more
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Catalog NumberQuantity
234561 Reaction
Catalog number 23456
Price (USD)/ Each
656.00
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Quantity:
1 Reaction
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Price (USD)/ Each
656.00
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The Thermo Scientific Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.

This crosslinking kit contains everything you need to help you insert an unbreakable molecular bridge between practically any two purified proteins and do it successfully right out of the box.

Features of the Controlled Protein-Protein Crosslinking Kit:

• Easy-to-control, stepwise coupling of two proteins—You are in charge of the chemistry. Stop and restart at convenient points or go to completion. It is your choice.
• Better protocol…logically presented and clearly worded instructions which will be appreciated by novice and expert—Instructions teach the method and chemistry of this crosslinking strategy to the user while providing a high likelihood of success. Great for life scientists attempting to prepare a conjugate or crosslink two proteins for the first time.
• Reliable non-cleavable heterobifunctional crosslinking agent—A very popular choice for a crosslinking agent was selected for the kit offering proven chemistry ,highly stabile intermediates and efficient formation of the target conjugate adding to the ease of use and likelihood of success.
• All reagents necessary to affect the crosslinking—Cuts costs over purchasing 'catalog size' packaging of all the components by more than 60%. If it's not in the kit, you don't need it!
• Includes disulfide (S-S) reductants and thiolation reagents—Allows for all possibilities regarding the state of the—SH group on one of the proteins to be coupled.
• Ellman's Reagent colorimetric—SH group determination chemistry built into the protocols—You have the option to see how well your reactions went. eg. determine the extent of—SH introduction or estimate the incorporation of maleimide reactive group.

Related Products
Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityNo
DescriptionControlled Protein-Protein Crosslinking Kit
FormKit
Labeling MethodChemical Labeling
Molecular Weight (g/mol)436.37
PEGylatedNo
Product LinePierce™
Quantity1 Reaction
Reactive MoietyMaleimide, Sulfo-NHS Ester
Shipping ConditionAmbient
SolubilityWater
Spacer Arm Length11.6 Å
Water SolubleYes
Chemical ReactivityAmine-Sulfhydryl
CleavableNo
Crosslinker TypeHeterobifunctional
FormatKit
Product TypeCrosslinker
SpacerShort (<10 Å)
Unit SizeEach
Contents & Storage
Sufficient For: One conjugation with two purified proteins (up to 5 mg each)
• Sulfo-SMCC Crosslinking Agent, 2 mg
• Activation/Conjugation Buffer (10X), 20 mL
• PBS packs (each makes 500mL), 2 packs
• 2-Mercaptoethylamine-HCl, 6 mg
• Immobilized-Reductant, 0.2 mL
• SATA, 2 mg
• Dimethylformamide (DMF), 1 mL
• Hydroxylamine-HCl, 5 mg
• Dextran Desalting Column, 2 x 10 mL
• Column Extender, 2 ea.
• Cysteine-HCl, 20 mg
• Ellman's Reagent, 2 mg

Store at 4°C.