Thermo Scientific™

Pierce™ Controlled Protein-Protein Crosslinking Kit

Catalog number:  23456

 Related applications: Protein Labeling & Crosslinking

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The Thermo Scientific Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.

This crosslinking kit contains everything you need to help you insert an unbreakable molecular bridge between practically any two purified proteins and do it successfully right out of the box.

Features of the Controlled Protein-Protein Crosslinking Kit:

• Easy-to-control, stepwise coupling of two proteins—You are in charge of the chemistry. Stop and restart at convenient points or go to completion. It is your choice.
• Better protocol…logically presented and clearly worded instructions which will be appreciated by novice and expert—Instructions teach the method and chemistry of this crosslinking strategy to the user while providing a high likelihood of success. Great for life scientists attempting to prepare a conjugate or crosslink two proteins for the first time.
• Reliable non-cleavable heterobifunctional crosslinking agent—A very popular choice for a crosslinking agent was selected for the kit offering proven chemistry ,highly stabile intermediates and efficient formation of the target conjugate adding to the ease of use and likelihood of success.
• All reagents necessary to affect the crosslinking—Cuts costs over purchasing "catalog size" packaging of all the components by more than 60%. If it's not in the kit, you don't need it!
• Includes disulfide (S-S) reductants and thiolation reagents—Allows for all possibilities regarding the state of the—SH group on one of the proteins to be coupled.
• Ellman's Reagent colorimetric—SH group determination chemistry built into the protocols—You have the option to see how well your reactions went. eg. determine the extent of—SH introduction or estimate the incorporation of maleimide reactive group.

Related Products
Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
For Research Use Only. Not for use in diagnostic procedures.


Crosslinker Type: Heterobifunctional
Labeling Method: Chemical Labeling
Product Line: Pierce™
Product Size: 1 reaction
Solubility: Water
Spacer: Short

Contents & storage

Sufficient For: One conjugation with two purified proteins (up to 5 mg each)
• Sulfo-SMCC Crosslinking Agent, 2 mg
• Activation/Conjugation Buffer (10X), 20 mL
• PBS packs (each makes 500mL), 2 packs
• 2-Mercaptoethylamine-HCl, 6 mg
• Immobilized-Reductant, 0.2 mL
• SATA, 2 mg
• Dimethylformamide (DMF), 1 mL
• Hydroxylamine-HCl, 5 mg
• Dextran Desalting Column, 2 x 10 mL
• Column Extender, 2 ea.
• Cysteine-HCl, 20 mg
• Ellman's Reagent, 2 mg

Store at 4°C.


Manuals & protocols