Thermo Scientific™

Neuron Isolation Enzyme (with papain) for Pierce™ Primary Cell Isolation Kits

Catalog number:  88285

 Related applications:

Primary Cell Culture

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Neuronal Isolation Enzyme (with papain) is a tissue-specific dissociation reagent for the isolation of primary cortical and hippocampal neurons from mouse/rat embryonic brain tissue and is a component of the Thermo Scientific™ Pierce™ Primary Neuron Isolation Kit (Product No. 88280).

Papain has been demonstrated to be more effective and less harsh than other proteases for tissue dissociation. When used with Cardiomyocyte Isolation Enzyme 2 (with thermolysin), it improves digestion efficiency and cell yield of primary cardiomyocytes.

Papain Information:

Source:Papaya latex
Molecular mass: 23kDa
Optimal pH: 6.0-7.0

More Product Data
An efficient method for the isolation of highly functional primary neurons

Related Products
Pierce™ Primary Neuron Isolation Kit
Neuronal Culture Medium for Pierce™ Primary Cell Isolation Kits
Neuronal Culture Media Supplement (50X) for Pierce™ Primary Cell Isolation Kits
N-PER™ Neuronal Protein Extraction Reagent
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Neuronal Cells (Pre-Natal), Primary Cells
Product Line: Pierce™
Product Size: 1 vial
Target Species: Mouse, Rat

Contents & storage

Stability: 1 year at -20°C
Storage of Reconstituted Enzyme: -20°C for 6 months and up to two freeze-thaw cycles, or 1 week at 4°C. Enzyme use after prolonged storage may result in poor performance.


Manuals & protocols