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Invitrogen
HuC/HuD Monoclonal Antibody (16A11)
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FIGURE: 1 / 7
HuC/HuD Antibody (A-21271) in ICC/IF
Immunofluorescent analysis of HuC/D (green) and MAP2 (red) on rat primary cortical neurons cultured for 28 days in the B-27 Plus Neuronal Culture System (Product # A3653401). At day 28 the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% triton x-100 for 30min, and blocked with 1% BSA for 30 min at room temperature. Cells were stained with anti-HuC/D antibody (Product # A-21271) at a dilution of 1:250, and anti-MAP2 (Product # PA5-17646) at a dilution of 1:250, in 1% BSA staining buffer, overnight at 4C, and th... View More
Product Details
A-21271
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Avian,
Chicken,
Human,
Zebrafish
Published species
Amphibian,
Avian,
Bacteria,
Cat,
Chicken,
Chimpanzee,
Fish,
Guinea pig,
Horse,
Human,
Lizard,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Reptile,
Rhesus monkey,
Rodent,
Shark,
Sheep,
Virus,
Xenopus,
Zebrafish
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
16A11
Immunogen
Human HuC/HuD neuronal protein.
Conjugate
Form
Lyophilized
Amount
100 µg
Purification
purified
Storage buffer
HEPES buffered saline
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.
Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20°C or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20°C, avoidig freeze/thaw cycles.
For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes.
A-21271 was successfully used to detect HuC/HuD in neurons differentiated from H9 ESC derived NSCs.
Target Information
Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV like neuron-specific RNA binding protein 3; ELAV like neuron-specific RNA binding protein 4; ELAV-like protein 3; ELAV-like protein 4; elav-related C; elav-related D; elavC; embryonic lethal, abnormal vision-related C; embryonic lethal, abnormal vision-related D; etID309927.3; Hu antigen C; Hu antigen D; Hu-antigen C; Hu-antigen D; HuC; HuD; Paraneoplastic cerebellar degeneration-associated antigen; paraneoplastic encephalomyelitis antigen; Paraneoplastic encephalomyelitis antigen HuD; Paraneoplastic limbic encephalitis antigen 21; Protein elrD; RNA-binding protein HuD; unnamed protein product
Gene Aliases: ELAVL3; ELAVL4; elrc; elrd; HUC; HUCL; HUD; id:ibd1248; PLE21; PNEM; wu:fb77b03; wu:fc24h01; zHuC
UniProt ID: (Human) Q14576, (Human) P26378, (Zebrafish) A0A0R4IEW8
Entrez Gene ID: (Human) 1995, (Chicken) 395634, (Human) 1996, (Zebrafish) 30732, (Zebrafish) 30737
nervous system development
cell differentiation
RNA processing
mRNA processing
learning
locomotory behavior
RNA splicing
cerebral cortex neuron differentiation
dendrite morphogenesis
3'-UTR-mediated mRNA stabilization
positive regulation of 3'-UTR-mediated mRNA stabilization
regulation of neurogenesis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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