iBright™ Imaging System Sample Stage/Turntable - FAQs

View additional product information for iBright™ Imaging System Sample Stage/Turntable - FAQs (A33829)

10 product FAQs found

Why do the iBright Imaging Systems use green LED illumination for DNA imaging?

The iBright Imaging Systems utilize a transilluminator based on green LEDs, which effectively excite popular DNA dyes such ethidium bromide and SYBR Green dye and offer several additional benefits:

- No harmful UV rays: While UV light effectively excites many fluorescent dyes and stains, UV light is a health hazard. Further, prolonged exposure to UV light can damage DNA samples, and compromise the integrity of samples to be used for downstream applications, such as subcloning.
- No mercury waste: UV transilluminator bulbs may contain mercury, a hazardous substance, and therefore require special care for handling and disposal.
- Longer lifetime: LED bulbs have a substantially longer real-time life than fluorescent UV bulbs, which can add up to considerable cost savings over the lifetime of the instrument.

For additional information on iBright functionality, visit this page.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How large is the sample imaging area on the iBright Imaging Systems?

The imaging area of the iBright Imaging system is 22.5 x 18.0 cm. This gives ample space for imaging 4 mini blots (or 2 midi blots) simultaneously.

For more information on iBright features and capabilities, visit this page.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the weight of the Thermal Printer for iBright Imaging Systems?

The Thermal Printer for iBright Imaging Systems weighs approximately 5.5 lbs (2.5 kg).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the dimensions of the Thermal Printer for iBright Imaging Systems?

Here are the dimensions of the Thermal Printer for iBright Imaging Systems:
Approximately 3.46 x 6.06 x 9.45 inches (88 x 154 x 240 mm).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the power consumption of the Thermal Printer for iBright Imaging Systems?

In stand-by mode, the power consumption of the Thermal Printer for iBright Imaging Systems is 10 Watts, and during the printing process, it is 115 Watts.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Which computer systems are compatible with the Thermal Printer for iBright Imaging Systems?

Windows 7/8/10 (32/64 bit) and MacOSX systems are compatible with the Thermal Printer for iBright Imaging Systems.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What type of paper do you recommend for the Thermal Printer for iBright Imaging Systems?

We recommend using UVP High Gloss Thermal Printer Paper (Fisher Cat. No. UVP89017401). It is 110 mm wide, with 220 to 245 prints per roll.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I image Ponceau staining best using an iBright imager?

We recommend taking Ponceau staining images in “Protein gel” mode, with the white screen. It is possible to play with the focus/resolution/sensitivity to get bands as sharp as possible. Better quality pictures are obtained when the membrane is wet.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How does the smart exposure technology work for an iBright imager?

Smart exposure technology rapidly determines optimal exposure time, which helps minimize the potential for over- or underexposed images, and the need to repeat exposures to get the desired signal. Smart exposure is enabled by built-in algorithms which aim to maximize sensitivity without creating band saturation. Smart exposure time varies depending on the gel imaged and bands displayed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the difference between the iBright CL1500 Imaging System and the iBright FL1500 Imaging System?

The iBright CL1500 Imaging System allows imaging of chemiluminescent Western blots, DNA and RNA gels stained with fluorescent nucleic acid stains, and visible protein gels. The iBright FL1500 Imaging System adds the capability of imaging fluorescent Western blots using multiple dyes.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.