iBright™ System Contrast Tray - FAQs

The iBright Imaging Systems support a wide range of dyes, stains, and fluorophores from the visible to NIR wavelength range.

For more information on compatible reagents and applications, visit this page.

To learn more about the iBright system-supported fluorophores and installed filters and light sources, visit Fluorescence SpectraViewer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The imaging area of the iBright Imaging system is 22.5 x 18.0 cm. This gives ample space for imaging 4 mini blots (or 2 midi blots) simultaneously.

For more information on iBright features and capabilities, visit this page.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The iBright CL750 Imaging System supports imaging of chemiluminescent western blots, colorimetric and fluorescent stained gels, and nucleic acid gels. It can also be used to image GFP- and crystal violet-stained colony plates.

For additional information about these applications or to learn more about the three iBright model capabilities, visit this page.

To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The iBright CL1500 Imaging System supports a wide range of applications including the following:

Protein gel: colorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain)

Nucleic acid gel: ethidium bromide and Invitrogen SYBR stains; imaging of E-Gels and hand pour gels is supported

Chemiluminescent western blot: chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)

Additional applications: colony counting for GFP, crystal violet, and blue/white colonies; TLC plates, and leaf sections

For additional information about these applications or to learn more about the three iBright model capabilities, visit this page.

To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The iBright FL1500 Imaging System provides maximum application support including up to 4-channel multiplex western blot imaging. See the list below for more details:

Protein gel: colorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain)

Nucleic acid gel: ethidium bromide and Invitrogen SYBR stains; imaging of E-Gels and hand-poured gels is supported

Chemiluminescent western blot: chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)

Fluorescent western blot: fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor and Alexa Fluor Plus conjugates)

Additional applications: colony counting for GFP, crystal violet, and blue/white colonies; TLC plates, and leaf sections

For additional information about these applications or to learn more about the three iBright model capabilities, visit this page.

To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The iBright System Contrast Tray is included with the purchase of a current iBright Imaging System, inclusive of the CL750, CL1500, and FL1500 iBright Imaging Systems. For additional information on iBright features and capabilities, visit the iBright Imaging Systems page.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

All iBright imager models are compatible with the orange iBright System Contrast Tray (Cat. No. A56597), inclusive of the CL1000, FL1000, CL750, CL1500, and FL1500 iBright imager models. For more information on compatible reagents and applications, visit the iBright Imaging Systems Compatible Reagents page.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

We recommend using the iBright System Contrast Tray (Cat. No. A56597) for imaging all visible protein gel samples such as those stained with Coomassie Blue or Silver Stain. For more information on compatible reagents and applications, visit the iBright Imaging Systems Compatible Reagents page.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The recommended imaging method depends on the iBright imager model being used. For iBright FL1500 and CL1500 Imaging Systems, we recommend imaging Ponceau-stained samples with the Ponceau application that can be found in Universal mode. For the iBright CL750 Imaging System, we recommend imaging Ponceau-stained samples in Protein gel mode.

We always recommend imaging with the orange iBright System Contrast Tray (Cat. No. A56597), and if needed, adjust the focus or zoom to get bands as sharp as possible. Better quality pictures are obtained when the membrane is wet.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.