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Invitrogen
SSEA4 Monoclonal Antibody (MC-813-70)
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FIGURE: 1 / 8
SSEA4 Antibody (MA1-021) in ICC/IF
Immunofluorescent analysis of SSEA-4 using anti-SSEA-4 monoclonal antibody (Product # MA1-021) shows staining on the cell surface of human H9 human embryonic stem cells, indicating pluripotency. SSEA-4 staining (green) and an overlay image of SSEA-4 with DAPI (blue) is shown. H9 human embryonic stem cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing SSEA-4 (Product # MA1-021) at a dilution of 1:40 overnight at 4 °C, washed with PBS and i... View More
Product Details
MA1-021
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Deer,
Human
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
MC-813-70
Immunogen
human embryonal carcinoma cell line 2102Ep
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
potassium phosphate with 0.15M NaCl, 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: SSEA-4; stage-specific embryonic antigen 4
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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