New—The Qubit™ 4 Fluorometer can now measure RNA quality in addition to providing accurate quantitation of dsDNA, ssDNA, RNA & protein.
Invitrogen™

Qubit™ dsDNA HS Assay Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  Q32854

 Related applications: Nucleic Acid Quantitation

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Description

The Qubit dsDNA HS (High Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for dsDNA HS (High Sensitivity) quantitation?
• For 1–20 samples: use this Qubit dsDNA HS Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT dsDNA HS Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.
3. Also available as a "1X" dsDNA assay kit that provides reagent and buffer in a formulation that is stable as a ready-to-use solution for up to one year after preparation.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): dsDNA
For Use With (Equipment): Qubit® Fluorometer
Quantitation Range: 0.2-100 ng
Number of Reactions: 500 reactions
Product Line: Quant-iT™, Qubit™
Product Size: 500 assays
Green Features: Fewer resources used and less waste
Shipping Condition: Room Temperature

Contents & storage

Store reagent and buffer at room temperature (protect reagent from light). Store the standards at 2–8°C.

Note: 500 µL thin-walled PCR tubes are required but not included.

Documents

Manuals & protocols

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