Invitrogen™

iBind™ Flex Western Starter Kit, fluorescent detection

蛍光検出用のiBind Western Starter Kitには、iBind Flex Western Device、iBind Flexカード詳細を見る

製品番号（カタログ番号）	数量
SLF2002S	1 kit

製品番号（カタログ番号） SLF2002S

価格（JPY）

328,000

1 kit

1 kit

蛍光検出用のiBind Western Starter Kitには、iBind Flex Western Device、iBind Flexカード、iBind Flex蛍光検出溶液キット（FD）が含まれています。iBind Flex Western Deviceは、ウェスタンブロット処理（免疫検出）を自動化する、小型の卓上用デバイスです。iBind Flexカードに機械的圧力をかけることで、ブロッキング、抗体結合、および洗浄ステップ用の免疫検出溶液のシーケンシャルラテラルフロー（SLF）を生成します。iBind Flexカードのガラス繊維マトリックスにより、溶液が絶えず均一にメンブレン上を流れるため、抗原と抗体の相互作用が促進されます。

iBind Flex Western Deviceを使用すると、手順の開始時にすべての溶液が調製されてデバイスにローディングされます。SLFの動作により、以降のすべてのステップは電気やバッテリーなしで自動的に続行されます。ブロットは約2.5時間で最終検出ステップで使用可能になります。

iBind Western Systemsの詳細 ›

iBind Flex Western Deviceには、ユーザーが交換可能な3つのウェルインサートが付属しています。このシステムとiBind FlexカードにiBind Flex溶液キットを併せて使用する場合は、最大で1ミディブロット、2ミニブロット、または6垂直カットストリップを処理できます。iBind Flex蛍光検出（FD）溶液キットを併せて使用する場合は、1ミニブロットを処理できます。

研究用にのみ使用できます。診断用には使用いただけません。

数量1 kit

出荷条件室温

容量1ミディブロット、6垂直カットストリップブロット、2ミニブロット

検出法蛍光、化学発光、比色分析

使用対象（アプリケーション）Western Blot

Membrane Compatibilityニトロセルロース、PVDF

製品ラインiBind

Running Time∼2.5 hr.

スループット1 Midi Blot, 6 Vertically-Cut Strip Blots, 2 Mini Blots

タイプスターターキット

Unit Size1 kit

組成および保存条件

1個のiBind Flex Western Device、10枚のiBind Flexカード（室温で保存）、1個のiBind Flex蛍光検出（FD）ソリューションキット（2～8℃で保存）

よくあるご質問（FAQ）

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.