|Flow Cytometry (Flow)||1:50-1:500|
|Host / Isotype||Mouse / IgG3|
|Immunogen||human embryonal carcinoma cell line 2102Ep|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
MA1-021-D650 has been successfully used in ICC/IF and flow cytometry applications on human samples.
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: SSEA-4; stage-specific embryonic antigen 4
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