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Product Details
PMC9484
Product Specifications
Species
Mouse
Expression System
E. coli
Amino acid sequence
Mouse LIF recombinant protein contains 181 amino acids
Molecular weight
20 kDa
Class
Recombinant
Type
Protein
Purity
≥95% by SDS-PAGE
Endotoxin concentration
≤1 EU/µg
Activity
ED50 ≤ 1 ng/mL; determined by the dose-dependent production of IL-6 in M1 myeloid leukemic cells.
Conjugate
Form
Liquid
Purification
purified
Storage buffer
PBS with 0.02% Tween 20
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
Storage: Store at -20°C to -80°C for 12 months, 1 month at 4°C after reconstituted or 3 months when stored at -20°C to -80°C after reconstituting as directed.
Target Information
Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: D factor; Differentiation-stimulating factor; Leukemia inhibitory factor; LIF; myeloid leukaemia inhibitory factor
Gene Aliases: Lif
UniProt ID: (Mouse) P09056
Entrez Gene ID: (Mouse) 16878
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