Will glycerol pose a problem when purifying antibodies with the Pierce Antibody Clean-up Kit (Cat. No. 44600)?
When using the Pierce Antibody Clean-up Kit, glycerol may pose a problem for the spin desalting columns for buffer exchange, but not for the Melon Gel itself. If glycerol concentrations are high, the solution becomes viscous enough to exert a frictional drag during spin column chromatography and the performance and yield may suffer.
Please find a technical tip on using Melon Gel for cleaning up BSA or gelatin from commercial antibodies.
If the glycerol concentration is higher than 10%, dialysis may be considered to exchange the sample into the Melon Gel buffer. We have some dialysis devices suitable for small volume dialysis, e.g., the Slide-A-Lyzer MINI devices: see here. Alternatively, some of the smaller Slide-A-Lyzer dialysis cassettes can be used: see here.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
If the glycerol concentration is higher than 10%, dialysis may be considered to exchange the sample into the Melon Gel buffer. We have some dialysis devices suitable for small volume dialysis, e.g., the Slide-A-Lyzer MINI devices: see here. Alternatively, some of the smaller Slide-A-Lyzer dialysis cassettes can be used: see here.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.