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Gibco Efficient-Pro Media and Feeds System
Discover the future of mAb manufacturing
The Efficient-Pro System is an advanced, next-generation monoclonal antibody media and feed system designed to work optimally with CHO cell lines. The media and feeds are specifically formulated to pair together for use in key bioproduction cell lines.
Developed with chemically defined and animal origin-free formulations, Efficient-Pro Media and feeds have been designed to help you maintain batch-to-batch consistency. Additionally, through Thermo Fisher’s global network of harmonized facilities and manufacturing redundancy, you can reduce the risk of supply disruption, enabling you to focus on your process.
For CHO cell lines requiring insulin, the Gibco Efficient-Pro Medium (+) Insulin offers the same strong performance and consistency as Efficient-Pro Medium with the added convenience of containing recombinant human insulin. This feature aims to eliminate the need for manual insulin supplementation and reduce process variability. Paired with the Efficient-Pro feeds, the medium can support a broad range of insulin-dependent CHO cell lines.
Mix less, achieve more
Struggling to achieve optimal titers with insulin-dependent CHO cell lines?
Inconsistent expression and slow growth can hinder upstream productivity and delay timelines. That’s why Thermo Fisher Scientific developed Efficient-Pro Medium (+) Insulin—a ready-to-use, high-performance medium formulated to help boost titers by up to 61% and streamline protein production without the need for manual insulin supplementation.
Key differentiators of Efficient-Pro Medium (+) Insulin
- Boost titers by up to 61% in insulin-dependent CHO cell lines—engineered for performance
- Streamline upstream prep with pre-supplemented insulin—no manual addition required
- Scale with confidence using liquid or AGT formats, from bench to GMP
- Improve consistency and compliance with a chemically defined animal-origin-free (AOF) formulation
- Reduce your media reconstitution steps by more than 50% with AGT format
The Efficient-Pro product family is expanding
Help boost CHO-K1 GS titers with Efficient-Pro Feed 3 and Feed Enhancer
Achieve up to 146% higher titers with our two-part, chemically defined, animal origin–free feed system. Designed with advanced metabolomics and high-throughput screening, these next-generation solutions aim to optimize CHO-K1 GS productivity while helping to maintain critical product quality attributes.
Customer testimonials
Hear how MacroGenics efficiently scaled up their process to 500 L cGMP scale with the Efficient-Pro System
Learn how MacroGenics was able to efficiently develop a consistent and scalable process in under a year by partnering with Thermo Fisher Scientific and successfully transitioning to the use of the Efficient-Pro Media and Feed System.
See the Efficient-Pro System in action: A collaboration with Fresenius Kabi BioPharma
Discover how Fresenius Kabi BioPharma partnered with Thermo Fisher Scientific to overcome the challenges of biosimilar production. This collaboration highlights the power of Gibco media and the Efficient-Pro system in achieving stringent quality targets and optimizing mAb production.
Learn how Zurich University of Applied Sciences unlocked the secret to consistent scale-up success with the Efficient-Pro System
Find out how the Zurich University of Applied Science partnered with Thermo Fisher Scientific to overcome the challenges in scaling a bioproduction process. Learn more about how the use of the Efficient-Pro System with the Thermo Scientific DynaDrive Single-Use Bioreactor enabled them to efficiently develop a consistent and scalable mAb bioproduction process.
Hear how MacroGenics efficiently scaled up their process to 500 L cGMP scale with the Efficient-Pro System
Learn how MacroGenics was able to efficiently develop a consistent and scalable process in under a year by partnering with Thermo Fisher Scientific and successfully transitioning to the use of the Efficient-Pro Media and Feed System.
See the Efficient-Pro System in action: A collaboration with Fresenius Kabi BioPharma
Discover how Fresenius Kabi BioPharma partnered with Thermo Fisher Scientific to overcome the challenges of biosimilar production. This collaboration highlights the power of Gibco media and the Efficient-Pro system in achieving stringent quality targets and optimizing mAb production.
Learn how Zurich University of Applied Sciences unlocked the secret to consistent scale-up success with the Efficient-Pro System
Find out how the Zurich University of Applied Science partnered with Thermo Fisher Scientific to overcome the challenges in scaling a bioproduction process. Learn more about how the use of the Efficient-Pro System with the Thermo Scientific DynaDrive Single-Use Bioreactor enabled them to efficiently develop a consistent and scalable mAb bioproduction process.
Support the unique nutritional and metabolic requirements of your CHO-K1 GS cell line to help maintain high production
Efficient-Pro Media and Feed System—Designed to increase titers and enhance your mAb manufacturing workflow
The basal media, available with or without insulin, are high-performing monoclonal antibody cell culture solutions that can help boost the performance of your CHO cell line. When paired with our Efficient-Pro Feed 3 and Feed Enhancer, the complete medium and feed system can effectively improve titers in CHO-K1 GS cells.
Together, the media and cell-specific feeds can help increase titers, as shown in Figures 1 and 2. When pairing the Efficient-Pro Medium with Efficient-Pro Feed 3 and Feed Enhancer, the CHO-K1 GS average titer improved by up to 146% over other suppliers’ medium and feeds (Figure 1). In the case of an insulin-dependent CHO-K1 GS cell line, up to 61% improvement in average mAb titer was observed compared to other suppliers’ medium and feeds when pairing the Efficient-Pro Feed 3 and Feed Enhancer with the Efficient-Pro Medium (+) Insulin (Figure 2).
Click image to enlarge
Figure 1: Efficient-Pro Medium with Efficient-Pro Feed 3 and Feed Enhancer resulted in up to 146% higher CHO-K1 GS mAb titers compared to other suppliers’ media and feeds.
Click image to enlarge
Figure 2: Efficient-Pro Medium (+) Insulin with Efficient-Pro Feed 3 and Feed Enhancer resulted in up to 61% higher titers compared to other suppliers’ media and feeds in an insulin-dependent CHO-K1 GS cell line.
Designed to support CHO-K1 productivity and improved protein quality
Help increase CHO-K1 productivity to achieve your desired mAb quality profiles
The basal media, available with or without insulin, are high-performing mAb cell culture solutions that can help boost the performance of your CHO cell line. When paired with our Efficient-Pro Feed 1, the complete medium and feed system can effectively support enhanced specific productivity in CHO-K1 cells (Figure 3), while helping to achieve your mAb production critical quality attribute targets (Figure 4), including optimized glycosylation patterns, charge-variant profiles, and protein aggregation levels.
Click image to enlarge
Figure 3:Efficient-Pro Feed 1 showed comparable productivity to the competitor. Efficient-Pro Medium and Feed 1 maintained high viability and lower cell density indicating a higher specific productivity compared to the competitor.
Click image to enlarge
Figure 4:Improved charge variant profile of CHO-K1 cells with Efficient-Pro Feed 1. Using Efficient-Pro Medium with Feed 1 resulted in a preferred charge variant profile with a more neutral profile than the benchmark medium and feed.
Designed to improve your CHO-S/DG44 cell line productivity and protein quality
Increase your titers up to 44% and support improved mAb product quality with the Efficient-Pro System
The basal media, available with or without insulin, are high-performing mAb cell culture solutions that can help boost the performance of your CHO cell line. When paired with our Efficient-Pro Feed 2, the complete medium and feed system can effectively improve titers and mAb quality in CHO-S and DG44 cells.
Titers increased by up to 44% with Efficient-Pro Medium and Efficient-Pro Feed 2 compared to another supplier’s medium and feed (Figure 5), while supporting improved mAb product quality (Figure 6).
Click image to enlarge
Figure 5: Efficient-Pro Medium with Efficient-Pro Feed 2 resulted in 44% higher protein titer over the competitor. Efficient-Pro Medium and Feed 2 maintained viability above 90% during the 14-day run with comparable cell density to the competitor.
Click image to enlarge
Figure 6: Charge profile variant of CHO-S cells with Efficient-Pro Feed 2. Using Efficient-Pro Medium and Feed 2 resulted in a preferred charge variant profile with a more neutral profile than competitor medium and feed.
Streamline your mAb production with the Gibco Advanced Granulation Technology (AGT) media format
All components of the Efficient-Pro system are available in liquid format and our proprietary AGT format, which can help simplify reconstitution and streamline your mAb manufacturing workflow.
The AGT medium and feeds are pH and osmolality preadjusted and designed for rapid dissolution, facilitating accelerated media preparation with 50% fewer steps, and helping to reduce the risk of manual errors as well as time and labor costs.
For faster scale-up, try the Efficient-Pro system in our AGT dry media format. The AGT format offers simple reconstitution and animal origin free (AOF), chemically defined properties that help streamline regulatory processes.
- Efficient-Pro AGT Medium
- Efficient-Pro AGT Medium (+) Insulin
- Efficient-Pro AGT Feed 1
- Efficient-Pro AGT Feed 2
- Efficient-Pro AGT Feed 3
The Efficient-Pro Medium and Feeds are protein-free. It is recommended to maintain supplementation with growth factors, an anti-clumping agent (Pluronic), peptones, GS supplement, GlutaMAX, or L-Glutamine, if currently used in the control process.
Since the Efficient-Pro Feeds are nutrient-rich, a lower feed concentration may be sufficient, and a feed titration can help with process optimization.
Support the unique nutritional and metabolic requirements of your CHO-K1 GS cell line to help maintain high production
Efficient-Pro Media and Feed System—Designed to increase titers and enhance your mAb manufacturing workflow
The basal media, available with or without insulin, are high-performing monoclonal antibody cell culture solutions that can help boost the performance of your CHO cell line. When paired with our Efficient-Pro Feed 3 and Feed Enhancer, the complete medium and feed system can effectively improve titers in CHO-K1 GS cells.
Together, the media and cell-specific feeds can help increase titers, as shown in Figures 1 and 2. When pairing the Efficient-Pro Medium with Efficient-Pro Feed 3 and Feed Enhancer, the CHO-K1 GS average titer improved by up to 146% over other suppliers’ medium and feeds (Figure 1). In the case of an insulin-dependent CHO-K1 GS cell line, up to 61% improvement in average mAb titer was observed compared to other suppliers’ medium and feeds when pairing the Efficient-Pro Feed 3 and Feed Enhancer with the Efficient-Pro Medium (+) Insulin (Figure 2).
Click image to enlarge
Figure 1: Efficient-Pro Medium with Efficient-Pro Feed 3 and Feed Enhancer resulted in up to 146% higher CHO-K1 GS mAb titers compared to other suppliers’ media and feeds.
Click image to enlarge
Figure 2: Efficient-Pro Medium (+) Insulin with Efficient-Pro Feed 3 and Feed Enhancer resulted in up to 61% higher titers compared to other suppliers’ media and feeds in an insulin-dependent CHO-K1 GS cell line.
Designed to support CHO-K1 productivity and improved protein quality
Help increase CHO-K1 productivity to achieve your desired mAb quality profiles
The basal media, available with or without insulin, are high-performing mAb cell culture solutions that can help boost the performance of your CHO cell line. When paired with our Efficient-Pro Feed 1, the complete medium and feed system can effectively support enhanced specific productivity in CHO-K1 cells (Figure 3), while helping to achieve your mAb production critical quality attribute targets (Figure 4), including optimized glycosylation patterns, charge-variant profiles, and protein aggregation levels.
Click image to enlarge
Figure 3:Efficient-Pro Feed 1 showed comparable productivity to the competitor. Efficient-Pro Medium and Feed 1 maintained high viability and lower cell density indicating a higher specific productivity compared to the competitor.
Click image to enlarge
Figure 4:Improved charge variant profile of CHO-K1 cells with Efficient-Pro Feed 1. Using Efficient-Pro Medium with Feed 1 resulted in a preferred charge variant profile with a more neutral profile than the benchmark medium and feed.
Designed to improve your CHO-S/DG44 cell line productivity and protein quality
Increase your titers up to 44% and support improved mAb product quality with the Efficient-Pro System
The basal media, available with or without insulin, are high-performing mAb cell culture solutions that can help boost the performance of your CHO cell line. When paired with our Efficient-Pro Feed 2, the complete medium and feed system can effectively improve titers and mAb quality in CHO-S and DG44 cells.
Titers increased by up to 44% with Efficient-Pro Medium and Efficient-Pro Feed 2 compared to another supplier’s medium and feed (Figure 5), while supporting improved mAb product quality (Figure 6).
Click image to enlarge
Figure 5: Efficient-Pro Medium with Efficient-Pro Feed 2 resulted in 44% higher protein titer over the competitor. Efficient-Pro Medium and Feed 2 maintained viability above 90% during the 14-day run with comparable cell density to the competitor.
Click image to enlarge
Figure 6: Charge profile variant of CHO-S cells with Efficient-Pro Feed 2. Using Efficient-Pro Medium and Feed 2 resulted in a preferred charge variant profile with a more neutral profile than competitor medium and feed.
Streamline your mAb production with the Gibco Advanced Granulation Technology (AGT) media format
All components of the Efficient-Pro system are available in liquid format and our proprietary AGT format, which can help simplify reconstitution and streamline your mAb manufacturing workflow.
The AGT medium and feeds are pH and osmolality preadjusted and designed for rapid dissolution, facilitating accelerated media preparation with 50% fewer steps, and helping to reduce the risk of manual errors as well as time and labor costs.
For faster scale-up, try the Efficient-Pro system in our AGT dry media format. The AGT format offers simple reconstitution and animal origin free (AOF), chemically defined properties that help streamline regulatory processes.
- Efficient-Pro AGT Medium
- Efficient-Pro AGT Medium (+) Insulin
- Efficient-Pro AGT Feed 1
- Efficient-Pro AGT Feed 2
- Efficient-Pro AGT Feed 3
The Efficient-Pro Medium and Feeds are protein-free. It is recommended to maintain supplementation with growth factors, an anti-clumping agent (Pluronic), peptones, GS supplement, GlutaMAX, or L-Glutamine, if currently used in the control process.
Since the Efficient-Pro Feeds are nutrient-rich, a lower feed concentration may be sufficient, and a feed titration can help with process optimization.
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Media evaluation, analytics and custom development services
From antibodies to advanced therapies, collaborate with a knowledgeable and trusted pioneer of bioprocessing innovation, providing customized solutions that help empower you to meet your project goals. Take advantage of Gibco Media by Design Services to customize a catalog medium to meet your exact specifications. We also offer convenient media and feed panel evaluations, comprehensive bioproduction analytics services, and flexible full custom media development services.
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