Seminar
Jun 16, 2019 - Jun 20, 2019
University of Milano-Bicocca | Building U6 - Piazza dell'Ateneo, 1 - 20126 Milan, Italy
HPLC 2019

Access seminar, oral and poster presentations from HPLC 2019

Benefit from the exciting new developments, applications and workflows we shared at HPLC 2019 in Milan.

Register for access

Lunchtime seminars

Revolutionizing Chromatography with the Vanquish UHPLC

Tuesday, 18 June 2019 | 13:00-13:50 | Hall U6-01A, ground floor
Gunda Köllensperger, University of Vienna, Department of Chemistry, Austria
Carsten Paul, Thermo Fisher Scientific, Germering, Germany

This seminar shall demonstrate how the Thermo Scientific Vanquish UHPLC platform has delivered tangible user benefits and revolutionized liquid separations over the past five years, cumulating in the recent Vanquish Duo UHPLC systems. It concludes with a practical illustration of how Dual LC on the Vanquish Duo enables both metabolomics and lipidomics applications in parallel.

Increasing Method Throughput with Ultra-High Pressure Liquid Chromatography

Wednesday, 19 June 2019 | 13:00-13:50 | Hall U6-01A, ground floor
James P. Grinias, Rowan University, Department of Chemistry & Biochemistry, USA
Whitney Shatz, Principal Scientist, Genentech Research and Development Group, San Francisco, USA

Learn how a 10-to-20-fold improvement in throughput utilizing UHPLC, including a 20 second cycle time method for the separation of four compounds and maintaining a baseline resolution of 1.5 between all peaks can be achieved. Considerations for the validation of these high-throughput methods will also be discussed.

Oral presentations

 

17 June,  
11:10-11:25, Hall U6 - 08
OC 17, Session 3.1 Fundamentals -1, Column efficiency: 
Ultra-high-pressure ion chromatography with suppressed conductivity detection at 70 MPa using columns packed with 2.5 um particle
Christopher Pohl, Thermo Fisher Scientific
17 June,  
14:35-14:50, Hall U6 - 07

OC 34, Session 2.2. Ion exchange chromatography & MS:
Universal ion exchange chromatography separation platform
Shanhua Hin, Thermo Fisher Scientific

17 June, 15:05-15:20, Hall U6 - 07

OC 36, Session 2.2. Ion exchange chromatography & MS:
A new multi-attribute method—Ion exchange chromatography of biopharmaceutical proteins coupled directly to high resolution MS
Ken Cook, Thermo Fisher Scientific

18 June, 11:45-12:00, Hall U6 - 09 OC 115, Session 4.2 Pharmaceuticals and biopharmaceuticals: Effects of metal contamination caused by iron-free HPLC systems on peakshape and retention of drugs with chelating properties
Mauro De Pra, Thermo Fisher Scientific
18 June, 17:30-17:45, Hall U6 - 07 OC 150, Session 2.4 Glycomics & protein interactions:
Creation, implementation and transferability of a Glucose Unit (GU) library for HILIC-FLD analysis of APTS-labelled released N-glycans
Phil Widdowson, Thermo Fisher Scientific
20 June, 11:55-12:10, Hall U6 - 06 OC 281, Session 1.2 Quality control:
An end-to-end workfl ow for an automated peptide mapping identifi cation method aimed at routine QA/QC laboratories
Tom Buchanan, Thermo Fisher Scientific

 

Posters

 

19 June, 11:55-14:00

Applications

P13: Site-specifi c conjugation of metal-chelating polymers for radioimmunotherapy applications

17 June, 11:55-14:00

Application

P16: A universal mobile phase system for simple pH optimization of mobile phases used for ion exchange chromatography and hydrophobic interaction chromatography

17 June,  11:55-14:00

Applications

P20: Creation, implementation and transferability of a Glucose Unit (GU) library for HILIC-FLD analysis of APTS-labelled released N-glycans

17 June,  11:55-14:00

Applications

P31: UHPLC-UV coupled to a single quadrupole mass detector for confi rmation and quantitation of a genotoxic impurity in a drug sample

17 June, 11:55-14:00

Applications

P37: Charged aerosol detection: Factors affecting uniform analyte response

17 June, 11:55-14:00

Applications

P84: Charged aerosol detection and method transfer of compendial, including USP, methods

17 June, 15:35-17:00

Applications

P139: An empirical approach to predict separation of water-soluble vitamins using reversed-phase chromatography

17 June, 15:35-17:00

Applications

P141: Automated method development in HPLC for the quantitative determination of catechins in tea

17 June, 11:55-14:00

Applications

P149: Native amino acid analysis in wine by HILIC separation and detection with single quadrupole mass spectrometry

17 June, 15:35-17:00

Applications

P178: Extraction and quantifi cation of synthetic cathinones from human urine using SOLA SPE and HILIC-MS/MS

18 June, 10:10-11:10

Applications

P184: High-throughput comprehensive coverage of hydrophilic and hydrophobic metabolites in beer utilizing a dual separation/high resolution accurate mass spectrometry system

18 June, 15:45-16:35

LC-MS & hyphenation

P298: Tandem UHPLC operation for high-throughput LC-MS peptide mapping analysis

18 June, 15:45-16:35

LC-MS & hyphenation

P302: Robust extraction, separation and quantitation of structural isomer steroids from human plasma by SPE with LC-MS/MS detection

18 June, 15:45-16:35

LC-MS & hyphenation

P305: Reproducible chromatographic performance enables low ppm LC-MS detection of Host Cell Proteins (HCPs) in biotherapeutics

18 June, 15:45-16:35

LC-MS & hyphenation

P307: Automated proteolytic digestion for high precision peptide mapping and in-depth characterization of biotherapeutic proteins

18 June, 15:45-16:35

LC-MS & hyphenation

P308: Novel way to introduce the traditional salt based chromatography technique of ion exchange charged variant analysis of biopharmaceutical proteins into high resolution MS-CVA/MS a new multi-attribute method

19 June, 10:20-11:20

LC-MS & hyphenation

P345: Low-fl ow on-line 2D LC-MS hyphenated with HRAM MS for comprehensive proteome profi ling

19 June, 12:40-14:00

Fundamentals

P453: HPLC mobile phase packaging, performance and laboratory productivity

19 June, 12:40-14:00

Fundamentals

P466: Strategies for the transfer of liquid chromatography methods between different instruments

19 June, 12:40-14:00

Fundamentals

P479: Localised quantitative structure-retention relationship modelling for rapid method development in reversed-phase HPLC

 
Applications
Applications

Products

Scale up to more options: Columns in every format, media and size!

For Prep LC columns, one size does not fit all. Whether you need unique chemistries for difficult separations, XtendedLife columns for longer use, or budget-friendly solutions for routine separations, we deliver. Our full range of column hardware offers robust performance and our wide variety of chemistries makes it easy to scale-up from your current analytical method. Our new chiral LC phases can extend the benefits even farther—separating most chiral compounds easily.

   Preparative HPLC Columns Brochure

   Preparative HPLC Columns Specifications Sheet

Preparative HPLC Dolumns Method Transfer Tool

 


Obtain more answers from your liquid chromatography by using mass spectrometry and detect a variety of analytes of extended mass range with HESI or APCI.

Resources


Choose biphenyl LC columns for fast and reliable separation of critical pairs and isomers. They provide unique selectivity for aromatic and moderately polar analytes.


Three workflows. Two flow paths. One integrated UHPLC solution. Improve your productivity and sample knowledge for a faster return on investment.

Resources


Discover the unique ProPac WCX weak cation exchange (WCX) column designed for protein characterization including therapeutic proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), biosimilars and their charge variants.

Resources


Pharmaceutical scientists can now have confidence in the results gained from the ultra-trace analysis of critical polar N-containing compounds due to the ultra-low absorption surface. New inert glass vial is constructed of 1st hydrolytic class special 33 expansion glass to meet strict USPs and Pharmacopeias.

Resources


The Q Exactive HF-X MS rises to the occasion and sets new standards in sensitivity, performance, and productivity.

Resources


Allows any chromatographer to easily access mass spectrometry with a system that has unmatched low mass sensitivity, is simple-to-use and robust.

Resources


Separate your science from the status quo. Experience uncompromised UHPLC—with no trade-offs in performance, robustness and ease of use.

Resources


Make the invisible visible with a versatile, universal detector that adds a unique form of detection to the speed and resolution of your UPHLC system.

Resources


With the ability to use 4-μm particle size IC columns on high-pressure capable IC systems, you can finally choose between achieving even faster run times or better chromatographic resolution.

Resources


The Thermo Scientific nano and capillary systems are ideal for use as an integrated part of the proteomics workflow and seamlessly combine with our mass spectrometry systems

Resources


Our extensive family of products includes a variety of particle sizes and column designs to meet all separation needs, offering improved resolution, enhanced sensitivity, faster analysis and consistent performance.

Resources


From microsampling to headspace applications, you'll find the right vials and closures to match your application and autosampler requirements from our comprehensive portfolio.

Resources


Simplify your lab workflow with unified control, processing and data management for your chromatography/mass spectrometry instruments.

Resources


For all HPLC/UHPLC and LC-MS applications, each Fisher Chemical high-quality solvent undergoes rigorous quality assurance and testing measures.


Deliver robust reproducible gradients with the Thermo Scientific pH buffer platform that is applicable to a wide range of mAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

Lunchtime seminars

Revolutionizing Chromatography with the Vanquish UHPLC

Tuesday, 18 June 2019 | 13:00-13:50 | Hall U6-01A, ground floor
Gunda Köllensperger, University of Vienna, Department of Chemistry, Austria
Carsten Paul, Thermo Fisher Scientific, Germering, Germany

This seminar shall demonstrate how the Thermo Scientific Vanquish UHPLC platform has delivered tangible user benefits and revolutionized liquid separations over the past five years, cumulating in the recent Vanquish Duo UHPLC systems. It concludes with a practical illustration of how Dual LC on the Vanquish Duo enables both metabolomics and lipidomics applications in parallel.

Increasing Method Throughput with Ultra-High Pressure Liquid Chromatography

Wednesday, 19 June 2019 | 13:00-13:50 | Hall U6-01A, ground floor
James P. Grinias, Rowan University, Department of Chemistry & Biochemistry, USA
Whitney Shatz, Principal Scientist, Genentech Research and Development Group, San Francisco, USA

Learn how a 10-to-20-fold improvement in throughput utilizing UHPLC, including a 20 second cycle time method for the separation of four compounds and maintaining a baseline resolution of 1.5 between all peaks can be achieved. Considerations for the validation of these high-throughput methods will also be discussed.

Oral presentations

 

17 June,  
11:10-11:25, Hall U6 - 08
OC 17, Session 3.1 Fundamentals -1, Column efficiency: 
Ultra-high-pressure ion chromatography with suppressed conductivity detection at 70 MPa using columns packed with 2.5 um particle
Christopher Pohl, Thermo Fisher Scientific
17 June,  
14:35-14:50, Hall U6 - 07

OC 34, Session 2.2. Ion exchange chromatography & MS:
Universal ion exchange chromatography separation platform
Shanhua Hin, Thermo Fisher Scientific

17 June, 15:05-15:20, Hall U6 - 07

OC 36, Session 2.2. Ion exchange chromatography & MS:
A new multi-attribute method—Ion exchange chromatography of biopharmaceutical proteins coupled directly to high resolution MS
Ken Cook, Thermo Fisher Scientific

18 June, 11:45-12:00, Hall U6 - 09 OC 115, Session 4.2 Pharmaceuticals and biopharmaceuticals: Effects of metal contamination caused by iron-free HPLC systems on peakshape and retention of drugs with chelating properties
Mauro De Pra, Thermo Fisher Scientific
18 June, 17:30-17:45, Hall U6 - 07 OC 150, Session 2.4 Glycomics & protein interactions:
Creation, implementation and transferability of a Glucose Unit (GU) library for HILIC-FLD analysis of APTS-labelled released N-glycans
Phil Widdowson, Thermo Fisher Scientific
20 June, 11:55-12:10, Hall U6 - 06 OC 281, Session 1.2 Quality control:
An end-to-end workfl ow for an automated peptide mapping identifi cation method aimed at routine QA/QC laboratories
Tom Buchanan, Thermo Fisher Scientific

 

Posters

 

19 June, 11:55-14:00

Applications

P13: Site-specifi c conjugation of metal-chelating polymers for radioimmunotherapy applications

17 June, 11:55-14:00

Application

P16: A universal mobile phase system for simple pH optimization of mobile phases used for ion exchange chromatography and hydrophobic interaction chromatography

17 June,  11:55-14:00

Applications

P20: Creation, implementation and transferability of a Glucose Unit (GU) library for HILIC-FLD analysis of APTS-labelled released N-glycans

17 June,  11:55-14:00

Applications

P31: UHPLC-UV coupled to a single quadrupole mass detector for confi rmation and quantitation of a genotoxic impurity in a drug sample

17 June, 11:55-14:00

Applications

P37: Charged aerosol detection: Factors affecting uniform analyte response

17 June, 11:55-14:00

Applications

P84: Charged aerosol detection and method transfer of compendial, including USP, methods

17 June, 15:35-17:00

Applications

P139: An empirical approach to predict separation of water-soluble vitamins using reversed-phase chromatography

17 June, 15:35-17:00

Applications

P141: Automated method development in HPLC for the quantitative determination of catechins in tea

17 June, 11:55-14:00

Applications

P149: Native amino acid analysis in wine by HILIC separation and detection with single quadrupole mass spectrometry

17 June, 15:35-17:00

Applications

P178: Extraction and quantifi cation of synthetic cathinones from human urine using SOLA SPE and HILIC-MS/MS

18 June, 10:10-11:10

Applications

P184: High-throughput comprehensive coverage of hydrophilic and hydrophobic metabolites in beer utilizing a dual separation/high resolution accurate mass spectrometry system

18 June, 15:45-16:35

LC-MS & hyphenation

P298: Tandem UHPLC operation for high-throughput LC-MS peptide mapping analysis

18 June, 15:45-16:35

LC-MS & hyphenation

P302: Robust extraction, separation and quantitation of structural isomer steroids from human plasma by SPE with LC-MS/MS detection

18 June, 15:45-16:35

LC-MS & hyphenation

P305: Reproducible chromatographic performance enables low ppm LC-MS detection of Host Cell Proteins (HCPs) in biotherapeutics

18 June, 15:45-16:35

LC-MS & hyphenation

P307: Automated proteolytic digestion for high precision peptide mapping and in-depth characterization of biotherapeutic proteins

18 June, 15:45-16:35

LC-MS & hyphenation

P308: Novel way to introduce the traditional salt based chromatography technique of ion exchange charged variant analysis of biopharmaceutical proteins into high resolution MS-CVA/MS a new multi-attribute method

19 June, 10:20-11:20

LC-MS & hyphenation

P345: Low-fl ow on-line 2D LC-MS hyphenated with HRAM MS for comprehensive proteome profi ling

19 June, 12:40-14:00

Fundamentals

P453: HPLC mobile phase packaging, performance and laboratory productivity

19 June, 12:40-14:00

Fundamentals

P466: Strategies for the transfer of liquid chromatography methods between different instruments

19 June, 12:40-14:00

Fundamentals

P479: Localised quantitative structure-retention relationship modelling for rapid method development in reversed-phase HPLC

 
Applications
Applications

Products

Scale up to more options: Columns in every format, media and size!

For Prep LC columns, one size does not fit all. Whether you need unique chemistries for difficult separations, XtendedLife columns for longer use, or budget-friendly solutions for routine separations, we deliver. Our full range of column hardware offers robust performance and our wide variety of chemistries makes it easy to scale-up from your current analytical method. Our new chiral LC phases can extend the benefits even farther—separating most chiral compounds easily.

   Preparative HPLC Columns Brochure

   Preparative HPLC Columns Specifications Sheet

Preparative HPLC Dolumns Method Transfer Tool

 


Obtain more answers from your liquid chromatography by using mass spectrometry and detect a variety of analytes of extended mass range with HESI or APCI.

Resources


Choose biphenyl LC columns for fast and reliable separation of critical pairs and isomers. They provide unique selectivity for aromatic and moderately polar analytes.


Three workflows. Two flow paths. One integrated UHPLC solution. Improve your productivity and sample knowledge for a faster return on investment.

Resources


Discover the unique ProPac WCX weak cation exchange (WCX) column designed for protein characterization including therapeutic proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), biosimilars and their charge variants.

Resources


Pharmaceutical scientists can now have confidence in the results gained from the ultra-trace analysis of critical polar N-containing compounds due to the ultra-low absorption surface. New inert glass vial is constructed of 1st hydrolytic class special 33 expansion glass to meet strict USPs and Pharmacopeias.

Resources


The Q Exactive HF-X MS rises to the occasion and sets new standards in sensitivity, performance, and productivity.

Resources


Allows any chromatographer to easily access mass spectrometry with a system that has unmatched low mass sensitivity, is simple-to-use and robust.

Resources


Separate your science from the status quo. Experience uncompromised UHPLC—with no trade-offs in performance, robustness and ease of use.

Resources


Make the invisible visible with a versatile, universal detector that adds a unique form of detection to the speed and resolution of your UPHLC system.

Resources


With the ability to use 4-μm particle size IC columns on high-pressure capable IC systems, you can finally choose between achieving even faster run times or better chromatographic resolution.

Resources


The Thermo Scientific nano and capillary systems are ideal for use as an integrated part of the proteomics workflow and seamlessly combine with our mass spectrometry systems

Resources


Our extensive family of products includes a variety of particle sizes and column designs to meet all separation needs, offering improved resolution, enhanced sensitivity, faster analysis and consistent performance.

Resources


From microsampling to headspace applications, you'll find the right vials and closures to match your application and autosampler requirements from our comprehensive portfolio.

Resources


Simplify your lab workflow with unified control, processing and data management for your chromatography/mass spectrometry instruments.

Resources


For all HPLC/UHPLC and LC-MS applications, each Fisher Chemical high-quality solvent undergoes rigorous quality assurance and testing measures.


Deliver robust reproducible gradients with the Thermo Scientific pH buffer platform that is applicable to a wide range of mAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

Discover the best HPLC systems for your applications and budget

Use these tools to help you decide

HPLC Systems Selection Guide
Rank the top HPLC instruments for your applications based on your responses to simple pull-down menus.

HPLC System Total Cost of Ownership Calculator
Estimate your cost for purchasing and running Thermo Scientific HPLC and UHPLC systems and compare costs between instruments.

LC Method Transfer Calculator
Transfer methods from HPLC to UHPLC conditions and calculate the new gradient table, adjust sample volumes and run times if applicable.

Not sure which system is the right for you?

Contact Us

Connect with an expert to help you decide.

Contact us