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Choose from a menu of verified real-time PCR assays to build your own custom Applied Biosystems TaqMan SARS-CoV-2 Mutation Panel, and get the results you need today, while preparing you for what’s to come. This scalable solution lets you run a few or hundreds of samples to identify one or many mutations—all on your current real-time PCR instrumentation.

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Features of the TaqMan SARS-CoV-2 Mutation Panel include:

  • Customizable—build your own custom panel from a menu of verified real-time PCR assays that allows you to identify currently relevant SARS-CoV-2 mutations and adapt quickly as additional mutations and variants emerge
  • Convenient—use your current real-time PCR instrumentation to conduct follow-up testing of SARS-CoV-2 samples
  • Scalable—run a few or hundreds of samples to identify for one or many mutations
  • Unique, streamlined workflow—combines our gold-standard TaqMan SNP Genotyping Assays with a 1-step RT-PCR reaction; go from RNA to results in just over 1 hour


Product details

TargetsChoose from a menu of verified real-time PCR SNP assays to build your custom panel
Assay design

Sequence-specific forward and reverse primers to amplify the target sequence region. The reverse primer also primes reverse transcription of the SARS-CoV-2 genomic RNA sequences. Each assay includes two TaqMan minor groove binder (MGB) probes with nonfluorescent quenchers (NFQ):

  • One VIC dye labeled probe to detect the reference sequence
  • One FAM dye labeled probe to detect the mutation sequence
  • Available sizes375 reactions 
    1,250 reactions 
    Sample inputRNA extracted from SARS-CoV-2 samples with a CT value of less or equal to 30 
    Turn around time1 hour and 10 min from extracted RNA to results
    Recommended instrumentsAny real-time PCR instrument such as the Applied Biosystems 7500, 7500 Fast Dx, 7500 FastQuantStudio 5, and QuantStudio 7 systems 
    Recommended softwareQuantStudio Design and Analysis Software v2.5 or later with the Genotyping Analysis Module


    Workflow

    Performance

    Our menu of verified real-time PCR assays provide robust detection of SARS-CoV-2 mutations. Below are results (cluster plots) as viewed using QuantStudio Design and Analysis Software 2.5 with the Genotyping Analysis Module. These cluster plots show very clear discrimination between the wild type samples (red dots along the x-axis) and the mutation samples (blue dots along the y-axis).

    Training Support

    In this short, 2 video tutorial, you will learn how to:

    • Set up the instrument software for detecting the SARS CoV-2 mutation panel
    • Analyze the data with the Applied Biosystems™ Design and Analysis Software 2.5 or later with the Genotyping Analysis Module using the run files from all Applied Biosystems real-time PCR instruments

    Go to thermofisher.com/educationconnect and log into your account or create a new one! 

    Analysis software

    Use QuantStudio Design and Analysis Software v2.5 or later with the Genotyping Analysis Module.



    Ordering information

    Premier Assays are high demand assays for popular variants, such as those originating in India, the UK, and Brazil/Amazon. These assays come in one standard size of 1,250 reactions and have a shorter timeline of delivery than Additional Assays.

    MutationGeneAssociated variantsVariants nicknamesCatalog#Qty
    D215GSB.1.351South African variantA51823
    D614GSB.1.1.207, P.1, B.1.1.33, B.1.1.7, B.1.177, B.1.258, B.1.351, B.1.525, Mink VariantNigerian, UK, South African, and Brazilian / Amazonas variantsA51818
    delH69V70SB.1.1.7, B.1.258, B.1.525UK variantA51808
    delY144SB.1.1.7UK variantA51817
    E484KSP.1 ,B.1.1.33, B.1.351, B.1.525 South African and Brazilian variantsA51813
    E484QSB.1.617​, B.1.617.1​, B.1.617.3India variantA51820
    F888LSB.1.52520A/S:484K UK VariantA51827
    K417NSB.1.351South African variantA51814
    K417TSP.1Brazilian / Amazonas variantA51815
    L18FSP.1, B.1.351South African and Brazilian/Amazonas variant)A51830
    L452RSB.1.617​, B.1.617.1​, B.1.617.2​, B.1.617.3, B.1.429India and California variantsA51819
    N439KSB.1.258A51825
    N501YSP.1, B.1.1.7, B.1.351UK, South African, and Brazilian / Amazonas variantsA51812
    P681HSB.1.1.207, B.1.1.7Nigerian and UK variantsA51816
    P681RSB.1.617.1​, B.1.617.2​, B.1.617.3India variantA51822
    S13ISB.1.427California variantsA51828
    S477NSB.1.526New York variantA51824
    T20NS

    P.1

    Brazilian / Amazonas variant A51821
    V1176FSP.2BrazilianA51826

    Additional Assays are available but have longer delivery timelines than Premier Assays as these products are made-to-order.

    MutationGeneAssociated VariantsVariant
    nicknames
    Assay IDCatalog #
    A49785
    375 rxn
    Catalog #
    A49786
    1250 rxn
    A1708DORF1B.1.1.7UK variantCVYMJF3
    A222VSB.1.177 CVMFWVP
    A570DSB.1.1.7UK variantCVU62RC
    A701VSB.1.351South African variantCVZTDZZ
    D80ASB.1.351South African variantCV32Z6V
    F157LS CVRWEK3
    L242_244LSB.1.351South African variantCV2W7KX
    Q27stopORF8B.1.1.7UK variantCVRWEKG
    R246ISB.1.351South African variantCV7DPCP
    S982ASB.1.1.7UK variantCVXGPV6
    T716ISB.1.1.7UK variantCVWCWA9
    W152LSR.1 CVTZ76Z
    Y453FSB.1.1.298Mink variantCVNKRFM

    Premier Assays are high demand assays for popular variants, such as those originating in India, the UK, and Brazil/Amazon. These assays come in one standard size of 1,250 reactions and have a shorter timeline of delivery than Additional Assays.

    MutationGeneAssociated variantsVariants nicknamesCatalog#Qty
    D215GSB.1.351South African variantA51823
    D614GSB.1.1.207, P.1, B.1.1.33, B.1.1.7, B.1.177, B.1.258, B.1.351, B.1.525, Mink VariantNigerian, UK, South African, and Brazilian / Amazonas variantsA51818
    delH69V70SB.1.1.7, B.1.258, B.1.525UK variantA51808
    delY144SB.1.1.7UK variantA51817
    E484KSP.1 ,B.1.1.33, B.1.351, B.1.525 South African and Brazilian variantsA51813
    E484QSB.1.617​, B.1.617.1​, B.1.617.3India variantA51820
    F888LSB.1.52520A/S:484K UK VariantA51827
    K417NSB.1.351South African variantA51814
    K417TSP.1Brazilian / Amazonas variantA51815
    L18FSP.1, B.1.351South African and Brazilian/Amazonas variant)A51830
    L452RSB.1.617​, B.1.617.1​, B.1.617.2​, B.1.617.3, B.1.429India and California variantsA51819
    N439KSB.1.258A51825
    N501YSP.1, B.1.1.7, B.1.351UK, South African, and Brazilian / Amazonas variantsA51812
    P681HSB.1.1.207, B.1.1.7Nigerian and UK variantsA51816
    P681RSB.1.617.1​, B.1.617.2​, B.1.617.3India variantA51822
    S13ISB.1.427California variantsA51828
    S477NSB.1.526New York variantA51824
    T20NS

    P.1

    Brazilian / Amazonas variant A51821
    V1176FSP.2BrazilianA51826

    Additional Assays are available but have longer delivery timelines than Premier Assays as these products are made-to-order.

    MutationGeneAssociated VariantsVariant
    nicknames
    Assay IDCatalog #
    A49785
    375 rxn
    Catalog #
    A49786
    1250 rxn
    A1708DORF1B.1.1.7UK variantCVYMJF3
    A222VSB.1.177 CVMFWVP
    A570DSB.1.1.7UK variantCVU62RC
    A701VSB.1.351South African variantCVZTDZZ
    D80ASB.1.351South African variantCV32Z6V
    F157LS CVRWEK3
    L242_244LSB.1.351South African variantCV2W7KX
    Q27stopORF8B.1.1.7UK variantCVRWEKG
    R246ISB.1.351South African variantCV7DPCP
    S982ASB.1.1.7UK variantCVXGPV6
    T716ISB.1.1.7UK variantCVWCWA9
    W152LSR.1 CVTZ76Z
    Y453FSB.1.1.298Mink variantCVNKRFM


    Additional products needed

    Master mix


    Recommended controls

    Product 
    GeneArt DNA string or plasmid controls thermofisher.com/geneart​

    GeneArt plasmid positive controls are available for selected assays. Contact us ›