The Thermo Scientific Aquilos 2 Cryo-FIB is the latest generation of our cryo-DualBeam system. It is dedicated to the preparation of thin, electron-transparent lamellas for high-resolution cryo-electron tomography or MicroED of micro-crystals. 

With the Aquilos 2 Cryo-FIB, you can take a revolutionary step forward by automating steps in the workflow to produce multiple lamellas from specific programmed locations. The improved dedicated cryo-hardware will provide you with longer run times at ultra-low contamination rates. Discover how our latest cryo-FIB platform enables the determination of structures right inside the cell.

  • Automation: Run the system overnight, allowing it to automatically produce multiple lamellas for you with Thermo Scientific AutoTEM Software.
  • Lift-out: Target and extract your structure of interest with the Thermo Scientific EasyLift NanoManipulator, our high-precision cryo-lift-out solution.
  • 3D visualization: View the native subcellular structure below the surface using charging contrast and identify features for high-resolution tomography in cryo-TEM with Thermo Scientific Auto Slice and View Software.
Thermo Scientific Aquilos 2 Cryo-FIB cryo sample prep tool.
DualBeam FIB-SEM Microscopes

Aquilos 2 Cryo-FIB for Life Sciences

See inside the cell to discover your answer.


Key Features

Extended run time

Your samples remain at vitreous temperatures, even for overnight runs. Cryo-hardware and a fully rotatable cryo-stage protect the frozen samples from contamination and ensure they stay frozen at all times.

Automated batch cryo-lamella milling

Mark multiple points of interest, such as cells, for cryo-lamella milling. Cryo AutoTEM Software is a guided solution for automatically preparing multiple lamellas in unattended runs, increasing your productivity.

Cryo-lift-out lamella preparation

Prepare lamellas from specific targeted regions with nanometer-position accuracy. The high-precision EasyLift NanoManipulator option allows for extraction of site-specific regions, such as fluorescently labeled proteins, even from high-pressure frozen samples. These lamellas can subsequently be placed inside autogrids for cryo-TEM and further imaging of protein structures and protein networks.

3D visualization

With our Cryo-Auto Slice and View Software, you can acquire 3D images from vitrified samples by sequential milling and then imaging of the cross-section of a defined volume of the sample. This process will help you stop milling at the correct subcellular region to prepare your lamella before you observe the ultrastructural detail in your cryo-TEM using cryo-ET.

Accelerate and Advance service packages for Cryo-tomography

Accelerate and Advance service packages for Cryo-tomography will support your success from the moment your purchase your Thermo Scientific Aquilos 2 Cryo-FIB system.  From applications support to system remote monitoring to comprehensive maintenance, our unique blend of data-driven and hand-on services will help you quickly achieve your desired outcomes.

At M&M 2019, Dr. Alex Rigort, Product Marketing Manager, Thermo Fisher Scientific, presented the new features of the Thermo Scientific Aquilos 2 Cryo-FIB. This latest generation of our cryo-DualBeam system is dedicated to the preparation of thin, electron-transparent lamellas for high-resolution cryo-electron tomography or MicroED of micro-crystals.

 

Preparation of cells for cryo-electron tomography

The Thermo Fisher cryo-electron tomography workflow covers flash freezing cells to final 3D visualization. Interior cellular regions are imaged at nanometer-scale resolution and from cryo-lamellae precisely prepared with Aquilos 2 cryo-FIB.

Specifications

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Electron Optics
 
Accelerating voltage range: 200 V – 30 kV
Beam current range: 1.5 pA to 400 nA
Resolution (with cryo-stage): 1.6 nm at 30 kV, 2.6 nm at 2 kV (at room temperature), 6.0 nm* at 2kV (at cryo-temperature)
Resolution (with cryo-stage): 7.0 nm at 30 kV
Ion Optics
 
Source lifetime: 1300 hours
Voltage: 500 V to 30 kV
Beam current: 1.5 pA – 65 nA in 15 steps
Resolution (with cryo-stage): 7.0 nm at 30 kV
Cryo-stage
 
Tilt range at cryo (eucentric WD): -15° to +55°
XY range: 110 mm
Z range: 65 mm
Rotation: 360° (endless)
Cooldown time:  <20 minutes
Vacuum system
 
Chamber vacuum at room temperature:  <4e–4Pa
Chamber vacuum at cryo-conditions:  <8e–5Pa
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Applications

Infectious_Disease_Research_App_w274x180

Infectious Disease Research
 

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

Life_Sciences_Application_274x180_144DPI

Structural Biology Research
 

Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.

DrugDiscovery_Appl_274x180

Drug Discovery
 

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›


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To ensure optimal system performance, we provide you access to a world-class network of field service experts, technical support, and certified spare parts.

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