High-throughput enzyme assays

High throughput enzyme assays

Meet the Gallery Plus Enzyme Master - the first automated enzyme analyzer designed specifically for enzyme assay applications. Thanks to custom designed software, all reagent additions, incubations, and measurements are fully-automated, which saves time, eliminates manual error, and improves method robustness. And with reliable instrumentation, you can facilitate a simple method transfer from R&D to the QA/QC floor. With the flexibility and automation of the Gallery Plus Enzyme Master enzyme analyzer, you’re all set to meet your lab’s current needs—and your future needs, too.

Enzyme assays are laboratory methods that measure the rate of enzyme reactions (enzyme activity). These assays also help to develop enzyme discovery and study enzyme catalytic mechanisms. Enzyme activity and enzyme assay are vital for the study of enzyme kinetics and enzyme inhibition. Usually the assay is carried out by determining the enzyme activity with and without activation by an added coenzyme. The activity can be monitored by measuring changes in concentration of substrates or products during the reaction.

From method development to routine analysis, set it and forget it: Enzyme efficiency finally mastered

Break free from the limitations of UV-VIS spectrophotometric enzyme assay. The Gallery Plus Enzyme Master enzyme analyzer efficiently manages many different enzymes and measuring conditions—all in a single instrument that offers consistent and reliable performance.  You’ll appreciate fully-automated reagents, incubations, and measurements that ensure your method and results can be easily duplicated in other labs, and the time- and labor-savings speak for themselves.

Reliable enzyme assay using a suitable high-throughput screening (HTS) is crucial for enzyme discovery, enzyme engineering, enzyme production monitoring, and drug discovery processes.  Developing sensitive enzyme assays suitable for high-throughput screening requires identification of relevant enzyme and substrates forms, careful measurements of kinetic parameters, buffers, pH, and choice of a detection technology for the final assay. Method development starts with identifying the key method variables through design of experiment (DoE), which requires many sets of samples to be tested for enzyme activities.

See how the Gallery Plus Enzyme Master enzyme analyzer will automate your workflow and give you the ability to provide parallel optimal pH measurements, full traceability, and reliable method development and transferability.

Why is optimal pH critical in enzyme assay?

pH is a critical parameter in method development and routine enzyme assay measurement. pH affects enzyme activity and charge. pH also affects shape of the substrate so that the substrate can not bind to the active site can not be catalyzed to form the final product. All enzymes have an ideal pH value, which is called optimal pH. Under optimal pH conditions, each enzyme shows the maximum activity. Determination of the optimal pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes.

The Gallery Plus Enzyme Master enzyme analyzer with the electrochemistry measurement unit (ECM) allows the parallel determination of sample pH and makes sure that this critical parameter is measured for all the samples.

See how the Gallery Plus Enzyme Master enzyme analyzer will automate your workflow and give you the ability to provide parallel optimal pH measurements, full traceability, and reliable method development and transferability.See how the Gallery Enzyme Master enzyme analyzer will automate your workflow and give you the ability to provide parallel optimal pH measurements, full traceability, and reliable method development and transferability.

Measuring enzyme parameters using UV-VIS spectrophotometry can be slow-going. But an exciting evolution in efficiency has arrived. The Gallery Plus Enzyme Master is the first automated enzyme analyzer designed specifically for enzyme assay applications. The combination of robust hardware and new custom designed software offers a best-in-class solution to streamline enzyme method development. Reliable routine enzyme assay/activity testing provides trusted walkaway efficiency. Fully-automated reagents, incubations, and measurements ensure your method and results can be effortlessly duplicated in other labs with both time- and labor-savings.

High throughput enzyme assays

High-throughput enzyme assays

  • True direct read enzyme analyzer
  • Increased throughput with a sample capacity of up to 350 photometric tests/hour.
  • Optional ECM unit for parallel pH and conductivity measurement
  • Workflow-based operation suitable for all users’ levels
  • Built-in barcode readers for samples and reagents
Enzyme specific assay methods

Enzyme specific assay methods

  • Flexible method parameters for each enzyme type
  • Enzyme specific incubation temperature, incubation time, data collection rate
  • Easy method development and method transfer
  • Wide wavelength coverage: 340–880 nm
  • Wide incubation temperature from 25 °C-60 °C presents new application possibilities
User interface and traceable data management

User interface and traceable data management

  • User administration
  • Audit trail
  • Time stamped raw data for further processing
  • Built-in barcode readers for samples and reagents
  • Instrument validation using IQ Kit
  • LIMS connectivity
Superior temperature control

Superior temperature control

  • Smart incubation temperature
  • Quick start function – fast system warm up
  • Temperature overheating limits
  • Incubation time to the seconds
  • No edging effect
Unique low volume disposable Decacell

Unique low-volume disposable DECACELL

  • Real-time kinetic measurement
  • Superior temperature control
  • Reduced sample and reagent consumption: 2–240 μL
  • Lowest waste generation and disposal cost

 
 
 
Benefits

Confidence in your results

  • Precise temperature control
  • Temperature overheating limits
  • Accurate incubation time to the seconds
  • Superior temperature control and lack of edge effects

Reproducibility

  • Fully automated liquid handling (same, buffer and substrate addition, and mixing)
  • Disposable cuvettes (no carryover)
  • Smart incubation temperature

Parallel pH measurement

  • ECM module for parallel pH measurement
  • Facilitates method development
  • Up to 67 samples pH measurement per hour

Fully automated workflow

  • Easy, automated method development
  • Easy method transfer
  • Enzyme-specific method parameters all in one central place
  • Many enzymes, many substrates, many methods – all on a single instrument

Traceability

  • Time stamped raw data
  • Audit Trail
  • User administration

Confidence in your results

  • Precise temperature control
  • Temperature overheating limits
  • Accurate incubation time to the seconds
  • Superior temperature control and lack of edge effects

Reproducibility

  • Fully automated liquid handling (same, buffer and substrate addition, and mixing)
  • Disposable cuvettes (no carryover)
  • Smart incubation temperature

Parallel pH measurement

  • ECM module for parallel pH measurement
  • Facilitates method development
  • Up to 67 samples pH measurement per hour

Fully automated workflow

  • Easy, automated method development
  • Easy method transfer
  • Enzyme-specific method parameters all in one central place
  • Many enzymes, many substrates, many methods – all on a single instrument

Traceability

  • Time stamped raw data
  • Audit Trail
  • User administration

 
 
 
Gallery Enzyme Master enzyme analyzer customer testimonials

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Sofie Wendel, Senior Department Manager, Assay Technology & Analytics at Novozymes A/S

“Our enzyme assays were developed and standardized based on legacy discrete analyzers. Our continued collaboration with Thermo Scientific resulted in improved and customized software for enzyme assays, and we replaced all the legacy discrete analyzers with the new Gallery Enzyme Master enzyme analyzers. The Gallery Enzyme Master delivers an efficient solution to the challenges of enzyme analysis, providing a tight temperature control, generating robust results. A major advantage is that the Gallery Enzyme Master makes transferability across the globe easier by enabling distribution from a central method development lab.”
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Erica Fritse, Global QC Governance Manager at DSM

“When needing an alternative for our aging population of Discrete Analyzers, we decided to replace it with the new Thermo Scientific Gallery Plus. The Gallery Enzyme Master ensures that we can continue with temperature-controlled measurement of the activity of our enzymes. We use the Gallery Enzyme Master for R&D development and QC release on one easy-to-use, automated system. A big advantage is the central, global management of the measuring protocols. This makes assurance of the use of the latest updated methods easier than ever before.”

Get more from your enzyme method development process—starting with walkaway efficiency. Thermo Scientific Gallery enzyme analyzers are fully-automated, sample oriented random-access analyzers. Many different tests can be run simultaneously from a single sample. Gallery enzyme analyzers employ colorimetric endpoint and kinetic as well as turbidimetric and bichromatic reactions with or without sample blanking. The analyzer includes a combined sample and reagent disk for a maximum capacity of 108 samples and 42 reagents, with the ability to perform up to 350 tests/hour. The wavelength range covers filter configurations from 340 nm up to 880 nm. An optional, integrated ECM unit supports conductivity and pH measurements over a wide range. The ECM unitcan measure up to 67 samples/hour for pH parallelly with other photometric tests.

 

Meet the Gallery Enzyme Master enzyme analyzer – A walkaway solution

Automate labor-intensive and time-consuming enzyme assay analysis

Thermo Scientific Gallery Enzyme Master Discrete Analyzer Thermo Scientific Gallery Plus Enzyme Master Discrete Analyzer

Gallery Enzyme Master
enzyme analyzer

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Gallery Plus Enzyme Master
enzyme analyzer

Request quote ›

Includes a combined sample and reagent disk for a maximum capacity of 90 samples and 30 reagents, with the ability to run up to 200 tests/hour.

Photometric tests/hour

Accommodates 108 samples and 42 reagents in separate sample and reagent disks, with the capability to run up to 350 tests/hour.

30

Reagents capacity

42

45-90

Sample capacity

54-108

Yes

Optional ECM unit

Yes

340, 405, 420, 510, 520, 540, 575, 600, 660, and 700 nm

Dedicated filters for enzyme applications

340, 405, 420, 510, 520, 540, 575, 600, 660, and 700 nm

Yes

Optional filters available

Yes

25-60°C

Incubation temperature range

25-60°C

9 seconds

Data collection rate

9 seconds

98610004

With ECM unit: 98611004

Part number

98620004

With ECM unit: 98621004

Both Gallery Enzyme Master instruments provide an integrated platform for electrochemical (pH and conductivity) measurements which can be run simultaneously with other photometric measurements.

Enzyme analysis at-a-glance

Perform robust, and reproducible enzyme assay with automated Gallery Enzyme Master discrete analyzer. It efficiently manages many different enzymes and many different measuring conditions—all in a single instrument that offers consistent, reproducible performance.

 

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