Biochemical Kinase Assays

Thermo Fisher Scientific offers you the broadest set of biochemical fluorescent assays to address high-throughput screening, hit confirmation and lead optimization programs.  All Invitrogen kinase assay technologies are homogenous, non-radioactive, and highly miniaturizable – and are available as kits, custom or bulk reagents.

For quality kinase profiling data on rapid timelines, choose SelectScreen, the industry’s most utilized kinase profiling service.

To compile the components needed to make the right biochemical kinase assay for your research, use our new Drug Discovery Assay Maker web tool.

See the LanthaScreen Kinase Activity Assay Reactivity Table

Request assay information        Use assay maker

Choose the right biochemical HTS kinase assay for your research?

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  Compatible with fluorescently labeled whole protein substrates Ideal for kinetic studies including on/off rates or for kinases with no known substrate Validated specifically for lipid kinases; can use with unmodified peptide or lipid substrates Primary assay for SelectScreen Kinase Profiling Service
  LanthaScreen Activity Assay LanthaScreen Eu Kinase Binding Assay Adapta Assay Z'-LYTE Assay
Application Technology basis Phospho-antibody TR-FRET Tracer-based TR-FRET ADP detection by TR-FRET FRET peptide phosphorylation
Application Primary screening Unactivated or low-activity kinase target screening Primary and secondary screening Secondary screening, hit validation, and profiling
Number of kinases validated 200 over 320 20 233
Readout Endpoint Endpoint or Kinetic Endpoint Endpoint
Total assay volume 20 µL 15 µL 15 µL 20 µL
ATP concentration range Tested up to 1 mM ATP not used Tested from 10 to 100 µM Tested up to 1 mM
Substrate type Peptide or protein No substrate needed Peptide, protein or lipid Peptide
Total assay volume Substrate concentration range 100 nM–1 µM Flexible; must be greater than ATP concentration 2 µM only
Plate signal stability Up to 18 hours Up to 24 hours Up to 18 hours 4–18 hours (kit-dependent)
Cell lysate compatible      
Overcoming interference Time-resolved readout allows read after interfering signals have decayed Time-resolved readout allows read after interfering signals have decayed; use of epitope-tag ensures no contaminating kinase is detected Time-resolved readout allows read after interfering signals have decayed; red Alexa Fluor dye as acceptor further enhances ability to overcome interference Preread at 445/520 nm
Cost per well $–$$ $–$$ $$–$$$ $$
Total assay volume  

Optimize with multiple high-throughput assay formats

Thermo Fisher Scientific offers four high-throughput assay technologies for your biochemical kinase research.

  • LanthaScreen Eu Kinase Binding Assays—Obtain sensitive detection of kinase inhibitors, including allosterics; interrogate active and non-activated kinases; track slow-binding inhibitors in real time, and compare inhibitor on-off rates with this robust assay technology.
  • LanthaScreen Kinase Activity Assays—A powerful toolbox for TR-FRET kinase activity assay development – choose exactly the reagents you need and acquire high quality data with minimal assay interference.
  • Adapta Universal Kinase Assays—A universal TR-FRET assay suitable for all kinases; including difficult-to-assay targets such as lipid kinases.
  • Z´-LYTE Kinase Assay—An ideal assay for secondary screening, hit validation and profiling, Z’-LYTE is the primary assay technology utilized in our SelectScreen Kinase Profiling Service.

Lower Throughput Assay Technologies

  • NDP Sensor—A universal, real-time kinetic kinase assay platform at a very low price per well
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