Evolution Spectrophotometer family

Meet today’s demands head on with a solution-based approach

Our next generation Thermo Scientific Evolution UV-Vis Spectrophotometers combine reliable and versatile hardware with easy-to-learn, easy-to-use Thermo Scientific Insight Pro Software. Together they will meet the diverse needs your organization requires from an analytical tool.

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Meet today’s demands head on with a solution-based approach
Reliable, versatile hardware
  • Address your laboratory’s needs with fixed or variable bandwidth options
  • Meet chemical, materials science, or biological sampling and measurement needs with a broad range of accessories
  • All accessories from previous Evolution UV-Vis Spectrophotometer models are compatible with today’s Evolution Spectrophotometers
A software partner
  • Meet current 21 CFR part 11 compliance, including database storage
  • Validate according to current USP, EP, or JP standards
  • Streamline your routine, complex, and advanced routines and workflows
Choose the instrument that’s right for you

Evolution One Spectrophotometer

Evolution One Plus Spectrophotometer

Evolution Pro Spectrophotometer

 Evolution One Spectrophotometer
Evolution One Plus Spectrophotometer
Evolution Pro Spectrophotometer
 Routine measurements, high resolution dataGreater experimental flexibility for research and more complex applicationsComprehensive solution for advanced applications
ApplicationQC applicationsComplex applications, research, kineticsHigh value QA/QC, advanced research


Spectral bandwidthFixed: 1 nmVariable:1 nm, 2 nm
AFBG Microcell optimized;
AFBG Fiber optic optimized;
AFBG Materials optimized
Variable: 0.5, 1, 1.5, 2, 4 nm
Light sourceXenonXenonXenon
Optical systemDouble beamDouble beamDouble beam


Current Pharmacopoeia complianceUSP and EPUSP and EPUSP, EP, and JP
21 CFR part 11*
Database storage
Report printing

Featured accessory compatibility**

Mercury lamp
pre-installed option also available
Fiber optics 
Peltier and Sipper systems
Multi-cell options
Integrating sphere for transmittance and reflectance  
VeeMax® Variable Specular Reflectance    
Praying Mantis Diffuse Reflectance  
Warranty2 years2 years2 years

*Requires Thermo Scientific Insight Pro Security Software
**Optional accessories are sold separately


Standards guide
Validated UV-Vis solution for pharmaceuticals

Evolution UV-Vis Spectrophotometers meet all USP and EP performance characteristics and enable 21CFR Part 11 compliance.  

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Pharmacopeia Compliance Specifications

Please note: Evolution One/One Plus meets current USP and EP compliance | Evolution Pro meets current USP, EP, and JP compliance.

ParametersStandard(s) usedPharmacopeia specification (tightest)
Wavelength accuracyMercury lampUSP - ≤ ± 1 nm in UV range ≤ ± 2 nm in visible range
JP - ≤ ± 0.3 nm over full λ range
Holmium perchlorate solutionUSP - ≤ ± 1 nm in UV range ≤ ± 2 nm in visible range
Didymium solution or glassUSP - ≤ ± 1 nm in UV range ≤ ± 3 nm in visible range
JP - ≤ ± 0.5 nm over full λ range
Wavelength repeatabilityMercury lampJP - ≤ ± 0.2 nm over full λ range USP - ≤ ± 0.5 nm over full λ range
Holmium perchlorate solutionEP/USP - ≤ ± 0.5 nm over full λ range
Didymium solution or glassJP - ≤ ± 0.2 nm over full λ range
Photometric accuracyCertified potassium dichromate (K2Cr2O7)EP - ± 0.010A USP - ≤ ± 0.010A in UV range ≤ ± 0.008A in visible range
Certified filtersEP - EP - ≥1.0A, ±0.8%A; <1.0A, ±0.008ª
JP - ± 1% of upper and lower limits of standard transmission rate value
Neutral density filters (Vis only)TGA
Photometric repeatabilityCertified potassium dichromate (K2Cr2O7)JP - For standard values ≤ 0.500, ±0.002 of mean; for standard values >0.500, ±0.004
Neutral density filters (Vis Only)JP - For standard values ≤ 0.500, ±0.002 of mean; for standard values >0.500, ±0.004
Stray lightCertified potassium chloride (KCl, 12g/L)EP - > 2.0A @ 198 nm (< 1%T)
Potassium iodide or sodium iodide (KI/NaI, 10g/L)USP/EP - > 3.0A @ 220 nm
AcetoneUSP - > 0.7A @ 300 nm
Sodium nitrite (NaNO2, 50g/L)EP- > 3.0A @ 340 nm
ResolutionToluene in hexane (0.02%)Ratio @ 0.5 SBW ≥ 2.3 at 25 °C
Photometric linearityCertified filtersJP/EP - Verify linearity of transmission rate, R2 > 0.999