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Evolution UV-Vis Spectrophotometers
Transform your UV-Visible experience with Thermo Scientific Evolution Spectrophotometers. Simplified workflows, advanced technology, and an array of accessories consistently deliver the high-quality results you depend on.
From simple measurements to sophisticated research studies, Evolution Spectrophotometers deliver reliable, accurate results. The simple user interface prompts users through the workflow, and complete solutions enable compliance with regulatory requirements.
Evolution UV-Vis Spectrophotometer features
Our next generation Evolution UV-Vis Spectrophotometers combine reliable and versatile hardware with easy-to-learn, easy-to-use Thermo Scientific Insight Pro Software. Together they will meet the diverse needs your organization requires from an analytical tool.
Meet today’s demands head-on with a solution-based approach
Reliable, versatile hardware
- Address your laboratory’s needs with fixed or variable bandwidth options
- Meets chemical, materials science, or biological sampling and measurement needs with a broad range of accessories, including those designed for previous Evolution models
A software partner
- Meets current 21 CFR part 11 compliance, including database storage (optional software)
- Validate according to current USP, EP, or JP standards
- Streamline your simple, complex, and advanced routines and workflows
Evolution UV-Vis Spectrophotometer selection guide
Evolution Spectrophotometers incorporate a high-performance optical design, versatile Insight Pro Software packages, and the highest quality accessories for the most demanding applications.
Choose the instrument that’s right for you
| Evolution One Spectrophotometer | Evolution One Plus Spectrophotometer | Evolution Pro Spectrophotometer | |
|---|---|---|---|
 |
 |
 |
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| Routine measurements, high resolution data | Greater experimental flexibility for research and more complex applications | Greater experimental flexibility for research and more complex applications | |
| Hardware | |||
| Spectral bandwidth | Fixed: 1 nm | Variable:1 nm, 2 nm AFBG Microcell optimized; AFBG Fiber optic optimized; AFBG Materials optimized |
Variable: 0.5, 1, 1.5, 2, 4 nm |
| Light source | Xenon | Xenon | Xenon |
| Optical system | Double beam | Double beam | Double beam |
| Software | |||
| Current Pharmacopoeia compliance | USP and EP | USP and EP | USP, EP, and JP |
| 21 CFR part 11* | ✓ | ✓ | ✓ |
CUE (Customized User Environment software) |
✓ | ✓ | ✓ |
| Database storage | ✓ | ✓ | ✓ |
| Report printing | ✓ | ✓ | ✓ |
| Kinetics | ✓ | ✓ | ✓ |
| Featured accessory compatibility** | |||
| Mercury lamp | ✓ | ✓ | ✓ pre-installed option also available |
| Fiber optics | ✓ | ✓ | |
| Peltier and Sipper systems | ✓ | ✓ | ✓ |
| Multi-cell options | ✓ | ✓ | ✓ |
| Integrating sphere for transmittance and reflectance | ✓ | ||
| VeeMax® Variable Specular Reflectance | ✓ | ||
| Praying Mantis Diffuse Reflectance | ✓ | ||
| Warranty | 2 years | 2 years | 2 years |
*Requires Thermo Scientific Insight Pro Security Software
**Optional accessories are sold separately
Evolution UV-Vis Spectrophotometer specifications
Product |
Evolution One |
Evolution One Plus |
Evolution Pro |
|
Optical Design |
Double-beam with sample and reference cuvette positions; Czerny-Turner monochromator |
Double-beam with sample and reference cuvette positions; Application Focused Beam Geometry (AFBG); Czerny-Turner monochromator |
Double-beam with sample and reference cuvette/accessory positions; Modified Ebert monochromator |
|
Spectral Bandwidth |
1.0 nm |
Variable: 1.0 nm; 2.0 nm; AFBG Microcell optimized; AFBG Fiber optic optimized; AFBG Materials optimized |
Selectable 0.5, 1.0, 1.5, 2.0, 4.0 nm |
|
Light Source (Typical Lifetime) |
Xenon flash lamp (>5 years; longer if not using live display; |
|||
| Detector | Dual Silicon Photodiodes | Detector dual-matched silicon photodiodes | ||
| Scan Ordinate Modes | Absorbance % Transmittance % Reflectance Kubelka-Munk log (1/R) log (Abs) Abs*Factor Intensity |
Absorbance % Transmittance % Reflectance Kubelka-Munk log (1/R) log (Abs) Abs*Factor Intensity 1st–4th Derivative |
||
| Grating | Holographic, 1200 lines/mm, blazed at 250 nm | Holographic, 1200 lines/mm, blazed at 240 nm | ||
| Wavelength | Range | 190–1100 nm | ||
| Accuracy | ±0.5 nm (541.9 nm xenon, 546.1 nm mercury lines) ±0.8 nm (full range 190–1100 nm) |
±0.20 nm (546.07 nm Hg emission line) ±0.30 nm (190–900 nm) |
||
| Repeatability | ≤0.05 nm (546.1 nm mercury line, SD of 10 measurements) |
|||
| Scanning speed | <1 to 6000 nm/min; variable | Variable, up to 6000 nm/min | ||
| Data intervals | 10, 5, 2, 1.0, 0.5, 0.2, 0.1 nm | 10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm | ||
| Photometric | Range | >3.5 A | >4 A | |
| Display Range | -0.3 to 4.0 A | ±6 A | ||
| Accuracy—Instrument1 | 1A: ±0.004 A 2A: ±0.008 A Measured at 440 nm using neutral density filters traceable to NIST |
1A: ±0.004 A 2A: ±0.004 A 3A: ±0.006 A Measured at 440 nm using neutral density filters traceable to NIST |
||
| Repeatability1 | 1A: ±0.0002 A | 1A: ±0.0001 A | ||
| Noise | 0A: ≤0.00015 A 1A: ≤0.00025 A 2A: ≤0.00050 A 260 nm, 1.0 nm SBW, RMS |
0A: <0.00018 A 1A: <0.00022 A 2A: <0.00050 A 500 nm, 2.0 nm SBW, RMS |
||
| Drift (Stability) | <0.0005 A/hour 500 nm, 1.0 nm SBW, |
<0.0005 A/hour 500 nm, 2.0 nm SBW, |
||
| Stray Light | KCl, 198 nm: ≤1% T NaI, 220 nm: ≤0.05% T NaNO2, 340 nm: <0.05% T |
KCl, 198 nm: ≤0.4% T NaI, 220 nm: ≤0.032% T NaNO2, 340 nm: <0.01% T |
||
| Baseline Flatness | ±0.001 A (200–800 nm) 1.0 nm SBW, smoothed |
±0.001 A (200–800 nm) 2.0 nm SBW, smoothed |
||
| Dimensions (W × D × H) | 593 × 475 × 266 mm (23.3" × 18.7" × 10.6") |
609 × 526 × 270 mm (23.9" x 20.7" x 10.6") |
||
| Weight | 14.5 kg (32 lb) | 20 kg (44 lb) | ||
| Electrical supply | 100–240 V, 50–60 Hz, selected automatically 150 W maximum |
100–240 V, 50–60 Hz | ||
| Additional Specifications | ||||
1 When testing instrument performance, the specification used for pass/fail determination is the sum of the instrument specification listed here and the uncertainty in the calibration data for the filter, listed on the calibration certificate.
Evolution UV-Vis Spectrophotometer pharmacopeia compliance
For UV-Visible spectrophotometers in regulated laboratories, performance verification at installation and at set intervals thereafter is needed. National pharmacopeias with international reach define specific performance levels for various criteria in the pharmaceutical industry.
Note that Evolution One/One Plus models meet current USP and EP compliance and the Evolution Pro model meets current USP, EP, and JP compliance.
| Parameters | Standard(s) used | Pharmacopeia specification (tightest) |
|---|---|---|
| Wavelength accuracy | Mercury lamp | USP - ≤ ± 1 nm in UV range ≤ ± 2 nm in visible range JP - ≤ ± 0.3 nm over full λ range |
| Holmium perchlorate solution | USP - ≤ ± 1 nm in UV range ≤ ± 2 nm in visible range | |
| Didymium solution or glass | USP - ≤ ± 1 nm in UV range ≤ ± 3 nm in visible range JP - ≤ ± 0.5 nm over full λ range |
|
| Wavelength repeatability | Mercury lamp | JP - ≤ ± 0.2 nm over full λ range USP - ≤ ± 0.5 nm over full λ range |
| Holmium perchlorate solution | EP/USP - ≤ ± 0.5 nm over full λ range | |
| Didymium solution or glass | JP - ≤ ± 0.2 nm over full λ range | |
| Photometric accuracy | Certified potassium dichromate (K2Cr2O7) | EP - ± 0.010A USP - ≤ ± 0.010A in UV range ≤ ± 0.008A in visible range |
| Certified filters | EP - ≥1.0A, ±0.8%A; <1.0A, ±0.008ª JP - ± 1% of upper and lower limits of standard transmission rate value |
|
| Neutral density filters (Vis only) | TGA | |
| Photometric repeatability | Certified potassium dichromate (K2Cr2O7) | JP - For standard values ≤ 0.500, ±0.002 of mean; for standard values >0.500, ±0.004 |
| Neutral density filters (Vis Only) | JP - For standard values ≤ 0.500, ±0.002 of mean; for standard values >0.500, ±0.004 | |
| Stray light | Certified potassium chloride (KCl, 12g/L) | EP - > 2.0A @ 198 nm (< 1%T) |
| Potassium iodide or sodium iodide (KI/NaI, 10g/L) | USP/EP - > 3.0A @ 220 nm | |
| Acetone | USP - > 0.7A @ 300 nm | |
| Sodium nitrite (NaNO2, 50g/L) | EP- > 3.0A @ 340 nm | |
| Resolution | Toluene in hexane (0.02%) | Ratio @ 0.5 SBW ≥ 2.3 at 25 °C |
| Photometric linearity | Certified filters | JP/EP - Verify linearity of transmission rate, r2 > 0.999 |
Evolution UV-Vis Spectrophotometer ordering information
Description |
Part Number |
|---|---|
Evolution One UV-Vis Spectrophotometer |
|
Evolution One Plus UV-Vis Spectrophotometer |
|
Evolution Pro UV-Vis Spectrophotometer |
Evolution Family FAQ
The Evolution family consists of advanced UV-Vis spectrophotometers designed for research, pharmaceutical, biotechnology, materials science, and regulated laboratory environments.
Evolution instruments offer enhanced optical performance, expanded automation and solid sampling options, advanced software functionality, and regulatory compliance capabilities compared to the more routine-focused GENESYS family.
Evolution models operate from 1100-190 nm, enabling applications from nucleic acid quantification to materials characterization.
Evolution instruments are controlled using Insight™ Pro software, a PC software which supports advanced data acquisition, analysis, reporting, and compliance workflows.
Yes. When configured with Insight Pro is paired with the Thermo Scientific Security Suite package, Evolution instruments can support 21 CFR Part 11 requirements, including user management, audit trails, and secure data storage.
Yes. Available accessories include:
- Automatic multi-cell changers
- Sipper systems
- Autosampler
Yes. Evolution instruments support advanced kinetics, including multi-wavelength kinetics and time-based studies for enzymatic and reaction monitoring applications.
Yes. Insight Pro includes preconfigured methods for DNA, RNA, and protein quantification, including popular assays like Protein Bradford.
Performance verification is performed using built-in routines and certified standards according to the user guide and laboratory qualification procedures. Evolution instruments also comply with USP <857> and Ph. Eu. 2.2.2.7, and include performance verification testing for ensuring performance with those guidelines.
Alignment, internal calibration, and service procedures should be performed by authorized Thermo Fisher Scientific service personnel.
For Research Use Only. Not for use in diagnostic procedures.