Recombinant Rabbit Monoclonal Antibody Services

Recombinant rabbit monoclonal antibody development and production services

Invitrogen recombinant antibodies are rabbit monoclonal antibodies that are developed by immunizing animals, screening for functionality, and then cloning the immunogen-specific antibody genes into high-level mammalian expression vectors. The recombinant antibodies are then produced by large-scale cell culture and purified with Protein A.

Choose our Invitrogen custom antibody service to obtain recombinant antibodies with the following features:

  • Greater specificity and sensitivity compared to standard antibodies
  • Lot-to-lot consistency due to recombinant technology
  • Animal origin–free antibodies expressed in a mammalian cell system

Custom Antibody Request   Learn more about recombinant technology

Recombinant rabbit polyclonal vs. monoclonal antibodies

Recombinant rabbit antibodies can be developed either as a polyclonal antibody or as a monoclonal antibody. Need help in making the right choice or need more information? Please contact us to discuss the options in detail.

  Description Benefit Suggested applications
Recombinant Rabbit Polyclonal Polyclonal–clone library used for final production Broader utility across assays
  • Western blot (WB)
  • Immunoprecipitation (IP)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Recombinant Rabbit Monoclonal Monoclonal–one clone isolated from library used for final production Quantitative detection
  • ELISA (quantitative assays)
  • Flow cytometry (FC)
  • Chromatin IP (ChIP)
  • Neutralization experiments

Development phases for custom recombinant antibodies

Development phases for custom recombinant antibodies

Notes:

  • Antigens for recombinant antibody development can be purified proteins, standard peptides, or modified peptides (i.e. phosphorylated).
  • Phase II is only performed for monoclonal protocols; polyclonal development uses only Phases I and III.
  • Screening phases can be accomplished using either of these two protocols:
    • Standard development–all screening performed by Invitrogen antibody services
    • Collaborative development–screening performed by the customer and Invitrogen antibody services