Multiplex assays for higher throughput
Luminex multiplex assays are driven by xMAP technology, designed to simultaneously detect and quantitate multiple secreted proteins (e.g., cytokines, chemokines, and growth factors) or expressed genes. This high-throughput technology produces results comparable to conventional assays such as ELISA and qPCR with greater efficiency and throughput.
Luminex assays and instrumentation
Multiplex up to 65 protein targets from complex biological samples, with flexible options from preconfigured solutions to custom mixed panels.
Multiplex up to 80 RNA targets from a single lysate sample. Custom panels are available based on your genes of interest.
As a Luminex partner, the entire Luminex portfolio of multiplexing instruments can be obtained directly from Thermo Fisher Scientific.
How it works- Luminex xMAP Technology
Luminex xMAP (Multi-Analyte Profiling) technology enables scientists to measure multiple proteins in a single well. This technology combines advanced fluidics, optics, and digital signal processing with innovated microsphere technology to deliver multiplexed assay capabilities. Featuring a flexible open-architecture design, xMAP technology can be configured to perform a wide variety of bioassays quickly, cost-effectively, and accurately.
xMAP technology uses superparamagnetic 6.5 micron microspheres with a magnetic core and polystyrene surface. The beads are internally dyed with precise proportions of red and infrared fluorophores. The differing proportions of the red and infrared fluorophores result in 100 unique spectral signature microspheres which are identified by the Luminex xMAP detection systems. The conjugation of a distinct antibody to a distinct bead allows for analysis of multiple analytes in a single well. Multiple analyte-specific beads can then be combined in a single well of a 96- or 384-well microplate- to detect and quantify multiple targets simultaneously, using one of the Luminex instruments for analysis.
The magnetic properties of the beads provide significant workflow advantages as it relates to washing. The included flat-bottom plates are compatible with either a hand-held magnetic plate washer (Cat. No. EPX-55555-000) or an automated magnetic plate washer.
Learn how to multiplex protein and gene expression on the Luminex platform from a single sample
Discover how multiplexing can be used to examine secreted proteins and mRNA expression levels on a single Luminex platform using a single sample. This publication in Methods specifically details how the ProcartaPlex and QuantiGene Plex assays can be paired to measure both proteins and messenger RNA (mRNA) expression in a single sample run on a Luminex platform.
Resources
Biomarker quantitation assay guide
Find detailed information about the different tools for protein and RNA quantitation including single analyte and multiplex assays. This valuable technical resource covers technologies useful for cancer, inflammation research, immunology, neurobiology, and more.
For Research Use Only. Not for use in diagnostic procedures.
