Luminex 200 Sampling Probe

Luminex xMAP technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. The Luminex xMAP systems are comprised of 4 instrument platforms—INTELLIFLEX, Luminex 200 (LX200), FLEXMAP 3D and MAGPIX (discontinued). These instruments utilize analyzers based on the principles of fluorescent flow cytometry or quantitative fluorescent microscopy and are designed to meet the needs of any size research laboratory. The Invitrogen ProcartaPlex and QuantiGene Plex assays are compatible with all 4 Luminex instrument platforms.


Luminex instruments overview

INTELLIFLEX System

INTELLIFLEX

Advanced Technology with a small footprint. This latest multifunctional multiplexing unit can perform up to 80 test and is ideally suited for high throughput applications (96- to 384-well). Advancements include upgraded hardware, a dual-reporter funtionality and side-eject for plates enabling ease of automation.

INTELLIFLEX
INTELLIFLEX DR-SE

Luminex 200 System

Luminex 200

The industry standard. The standard instrument for multiplexing able to perform up to 80 different tests in a single reaction volume. The instrument is flow cytometry-based and capable of delivering fast and cost effective results.

FLEXMAP 3D System

FLEXMAP 3D

Speed and throughput. This multiplexing unit can perform simultaneous measurement of up to 80 tests in a single reaction volume and is compatible for both 96-well and 384-well plates.

MAGPIX System

MAGPIX

Simple and compact. This multiplexing unit performs up to 50 different tests in a single reaction volume with a plate read time of 60 minutes and is based on CCD imaging technology.


Comparison of Luminex instruments

 
xMAP
INTELLIFLEX DR-SE


xMAP
INTELLIFLEX
FLEXMAP 3D System

FLEXMAP 3D

Luminex 200 System

Luminex 200
Detection technologyFlow cytometry-based
Lasers, APDs, PMTs
Footprint including PC (linear bench space)58.4 cm x 61 cm x 76.2 cm58.4 cm x 61 cm x 76.2 cm  110 cm x 62 cm x 63 cm115 cm x 60 cm x 50 cm
Weight (analyzer)54.5 kg (120 lbs)54.5 kg (120 lbs)77.1 kg (170 lbs)49 kg (113 lbs)
MultiplexUp to 500Up to 500Up to 500Up to 100
(80 on MagPlex)
Sample volume6.3-80 uL(assay dependent)  
Plate format96-well/384-well96-well/384-well96-well/384-well96-well
Read time96-well in ~20 min96-well in ~20 min96-well in ~20 min45 min (96-well)
384-well in ~75 min384-well in ~75 min384-well in ~75 min
Sensitivity*
(assay dependent)
0.06-1 pg/ml (immunoassays)
1000 RNA copies per target/well (RNA assays)
Reporter laser532 nm (green) and 405 nm (violet)532 nm (green)532 nm (green)532 nm (green)
Dynamic range≥5.5 logs (RP1)
≥4.5 logs (RP2)
≥5.5 logs (RP1)≥4.5 logs (RP1)≥3.5 logs (RP1)
Operational validationCalibration/verification/fluidics
21 CFR compliance20212021YesYes
Automation20212021Integration softwareIntegration software
Analytics (software)​Thermo Fisher Connect Data Analysis Apps for Luminex assays​xPONENT Basic + Thermo Fisher Connect Data Analysis Apps for Luminex assays


Luminex instrument accessories

Accessory equipment and reagents are available for use with assays run on Luminex systems, including sheath fluid, calibration kits and performance verification kits. We are committed to ensuring that you have all the necessary components you need to run successful Luminex RNA & protein quantitation assays on MAGPIX, LX200, FLEXMAP 3D or INTELLIFLEX Luminex systems.

The Hand-Held Magnetic Plate Washer (96-well) is designed for fast and simple removal of supernatant from samples bound to magnetic particles. The Hand-Held Magnetic Plate Washer (96-well) is an ideal tool for use with Invitrogen singleplex and multiplex biological assays based on the Luminex xMAP Technology. These assays make use of Luminex MagPlex or magnetic microspheres, and the MAGPIX, LX200, FLEXMAP 3D or INTELLIFLEX instruments. For 384-well assay formats, we recommend using an automated magnetic plate washer for consistent and efficient bead washing.

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Luminex technology

Luminex instrumentation

Luminex instrumentation is comprised of four instrument platforms—INTELLIFLEX, LX200, FLEXMAP 3D and MAGPIX. These instruments are based on either the principles of quantitative fluorescent microscopy or fluorescent flow cytometry. The multiplex quantitation capability of an assay is dependent upon instrument model, and ranges from 50 analytes with use of the MAGPIX, to 500 analytes with the INTELLIFLEX and FLEXMAP 3D. Used in combination with Invitrogen QuantiGene Plex and ProcartaPlex assays, the Luminex platform delivers fast and cost-effective gene expression and immunoassay profiling analysis in both 96- and 384-well formats. This multiplex system allows for the simultaneously measurement of up to 80 different analytes for RNA or protein from a single microplate well.

The Invitrogen QuantiGene Plex and Procarta Plex assays utilize xMAP (multi-analyte profiling) technology to perform a wide range of protein- and nucleic acid-based multiplex assays. xMAP Technology uses labeled microspheres or beads, allowing for the simultaneous capture of multiple analytes from a single reaction. The beads are individually read using a Luminex xMAP instrument.

MAGPIX

The MAGPIX system is based on the principles of fluorescence imaging utilizing Light Emitting Diodes (LEDs) and a CCD camera. The compact MAGPIX instrument is capable of simultaneously measuring and quantitating up to 50 analytes in a single microtiter well when used with magnetic micropsheres (beads). The MAGPIX instrument has been discontinued and is no longer available for purchase but MAGPIX Calibration and Performance Verification kits as well as Drive Fluid PLUS for existing instruments will continue to be available. The Invitrogen ProcartaPlex and QuantiGene Plex assays are compatible with existing MAGPIX instruments.

LX200 and FLEXMAP 3D

The LX200 and FLEXMAP 3D reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 80 different sets of color-coded magnetic microsphere beads, each bearing a different assay. The Luminex reader is an essential tool that performs the key functions of this multiplex technology:

Procartiflex technology fluidics

Fluidics

The reader detects individual beads by flow cytometry. The fluidics system of the reader aligns the beads into single file as they enter a stream of sheath fluid and then enter a flow cell. Once the beads are in single file within the flow cell, each bead is individually interrogated for bead color (analyte) and assay signal strength (PE fluorescence intensity).


Reader lasers

Lasers

The reader uses a 532 nm green laser ("assay" laser) is used to excite the PE dye of the assay (streptavidin-PE). The 635 nm solid state laser (red "classify" laser) is used to excite the dyes inside the beads to determine their "color" or "region" and is also used for doublet discrimination by light scatter.


Reader detectors

Detectors

The reader has four detectors, one for each of the optical paths shown in the figure below. Detectors are used to measure the fluorescence of the assay, to make bead determination (1-100) and the last to discriminate between single and aggregate beads.
 

INTELLIFLEX

The Luminex xMAP INTELLIFLEX System is the newest addition to the xMAP line of instruments that combines lasers, fluidics, and real-time digital signal processing for multiplex readouts. It is available in two configurations, the INTELLIFLEX (Cat. No APX2020) and the INTELLIFLEX DR-SE (Cat. No APX2021). The key difference between these configurations is the availability of the dual reporter and side eject feature which are exclusively to the DR-SE configuration. The additional violet reporter laser and detector in the DR-SE configuration makes the INTELLIFLEX DR-SE the only Luminex xMAP instrument capable of measuring up to 2 parameters per bead region.

The Luminex xMAP INTELLIFLEX & INTELLIFLEX DR-SE feature updated fluidics, software and a reduced footprint when compared to the Luminex 200 and FLEXMAP 3D systems. With automated start up, shut down, and maintenance routines, the Luminex xMAP INTELLIFLEX systems are easy to use and maintain, providing fast read times for both 96- and 384-well plate formats. These systems are compatible with all Invitrogen ProcartaPlex multiplex immunoassays and Invitrogen QuantiGene Plex multiplex gene expression assays which are available in both 96- and 384-well plate formats for high throughput applications.


Hand-Held magnetic plate washer for magnetic bead assays

Hand-Held magnetic plate washer for magnetic bead assays

We have developed a proprietary Hand-Held Magnetic Plate Washer (Cat. No. EPX-55555-000, sold separately) to simplify the bead wash process.



xPONENT software

xPONENT software is a modular, flexible, software package for control of the MAGPIX, 200, and FLEXMAP 3D systems, offering:

  • Intuitive graphical user interface for simplified workflow
  • Automated startup, shutdown, and routine maintenance operations
  • Enhanced data analysis and report generation capabilities
  • 21 CFR Part 11 ready
  • Automation and LIS connectivity

xPONENT software benefits:

  • Modular and flexible—Designed for ease of use, xPONENT software allows novice, occasional, and advanced users set up and run assays in minimal amounts of time.
  • User customization—The default graphical user interface follows a typical assay workflow, and it can be customized to meet the needs of individual laboratories.
  • Powerful data analysis—Industry-standard curve fitting models for quantitative analysis, allelic ratios for genotyping, and qualitative algorithms for immunology assays in addition to data visualization tools.
  • xPONENT software support


Invitrogen Luminex multiplex assays

ProcartaPlex Multiplex Immunoassays

ProcartaPlex multiplex immunoassays

ProcartaPlex multiplex immunoassays enable simultaneous detection of up to 65 protein targets from complex biological matrices.

QuantiGene Plex Gene Expression Assays

QuantiGene plex gene expression assays

QuantiGene Plex gene expression assays enable multiplexing of up to 80 RNA targets from a single lysate sample.

Biomarker quantitation assay guide

This 72-page guide provides detailed information about different tools for protein and RNA quantitation. Download this valuable technical resource that covers technologies useful for cancer and inflammation research, immunology, neurology and more. Learn more about how antibody pairs, ELISA kits, and multiplex kits for the Invitrogen Luminex platform may help advance your research.

Download the Biomarker quantitation assay guide

Biomarker quantitation assay guide cover

Resources

Access Luminex instruments and multiplex assays and a targeted collection of resources for multiplex applications: