Luminex xMAP technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. This information is presented to support researchers who use MAGPIX, 100/200, and FlexMAP 3D Luminex systems.
If your Luminex instrument needs attention or you require technical assistance with your Luminex assays or xPONENT software for data acquisition and analysis, contact our Technical Support team or email LuminexFAS@thermofisher.com.
Luminex instruments overview
MAGPIX
Simple, affordable and compact. This multiplexing unit performs up to 50 different tests in a single reaction volume with a plate read time of 60 minutes. Based on CCD imaging technology it is exclusive for its compactness and easy to use operation procedure.
Luminex 200
The industry standard. The standard instrument for multiplexing able to perform up to 80 different tests in a single reaction volume. The instrument is flow cytometry-based and capable of delivering fast and cost effective results.
FLEXMAP 3D
Speed and throughput. Most advanced and multifunctional multiplex unit for simultaneous measurement of up to 80 tests in a single reaction volume, compatible also for 384-well plates.
 Efficient and compact in size |
 The versatile, efficient standard in multiplexing |
 High-throughput, multiplex, automation compatible |
|
|---|---|---|---|
| MAGPIX System | 200 System | FLEXMAP 3D System | |
| Optics | LED/CCD camera | Lasers/APDs/PMTs | Lasers/APDs/PMTs |
| Hardware | Fluorescent imager | Flow cytometry based | Flow cytometry based |
| Software | xPonent 4.2 | xPonent 3.1 | xPonent 4.2 |
| Applications | Protein/Nucleic Acid | Protein/Nucleic Acid | Protein/Nucleic Acid |
| Bead compatibility | Magnetic | Magnetic/polystyrene | Magnetic/polystyrene |
| Multiplex capacity | 50 | 100 | 500 |
| Read time (96-well plate) | ~ 60 min | ~ 40 min | ~ 20 min |
| Dynamic range | 3.5 logs | 3.5 logs | 4.5 logs |
| Sensitivity | Detect ≤ 700 PE fluorochromes per xMAP microsphere | Detect 1,000 PE fluorochromes per xMAP microsphere | |
| Microtiter plate | 96 well | 96 well | 96 and 384 well |
| Weight (Analyzer) | 17.5 kg(38.5 lbs) | 49 kg (113 lbs) | 77.1 kg (170 lbs) |
| Magnetic 96-well Separator | Cat. No. A14179 (compatible with MAGPIX, 200, and FLEXMAP 3D Systems) | ||
xPONENT software
xPONENT software is a modular, flexible, software package for control of the MAGPIX, 200, and FLEXMAP 3D systems, offering:
- Intuitive graphical user interface for simplified workflow
- Automated startup, shutdown, and routine maintenance operations
- Enhanced data analysis and report generation capabilities
- 21 CFR Part 11 ready
- Automation and LIS connectivity
xPONENT software benefits
- Modular and flexible—Designed for ease of use, xPONENT software allows novice, occasional, and advanced users set up and run assays in minimal amounts of time.
- User customization—The default graphical user interface follows a typical assay workflow, and it can be customized to meet the needs of individual laboratories.
- Powerful data analysis—Industry-standard curve fitting models for quantitative analysis, allelic ratios for genotyping, and qualitative algorithms for immunology assays in addition to data visualization tools.
- xPONENT software support
If you require technical assistance with your xPONENT software for data acquisition and analysis, contact our Technical Support team or email LuminexFAS@thermofisher.com.
Accessory equipment, reagents, and services are available for use with assays run on Luminex systems, including sheath fluid, calibration kits, power cords, and extended warranties. We are committed to ensuring that you have all the necessary components you need to run successful Luminex protein quantitation assays on MAGPIX, Luminex 200, or FlexMAP 3D Luminex systems.
The Magnetic 96-Well Separator is designed for fast and simple removal of supernatant from samples bound to magnetic particles and may be used manually or as an integrated part of an automated workstation. The Magnetic 96-Well Separator is an ideal tool for use in singleplex and multiplex biological assays based on the Luminex xMAP Technology. These assays make use of Luminex MagPlex or magnetic microspheres, and the MAGPIX, 200, or FLEXMAP 3D instruments.
Find additional accessories by category
Biomarker quantitation assay guide
This 72-page guide provides detailed information about different tools for protein and RNA quantitation. Download this valuable technical resource that covers technologies useful for cancer and inflammation research, immunology, neurology and more. Learn more about how antibody pairs, ELISA kits, and multiplex kits for the Invitrogen Luminex platform may help advance your research.
Connect with us
Visit this link to answer all your questions when new to our multiplex technologies or if you need to troubleshoot unexpected results. Additionally guides and tools including videos are available for easy learning.
Email us to receive one to one consultation with a Luminex Field Application Scientist who can provide answers to determining the right products, product use and recommendations for data analysis.
Performance guarantee
If an Invitrogen immunoassay does not perform as described on our data sheet, we will replace the product at no cost to you, or if you prefer, we will provide you with a credit for future purchase.*
Learn more about performance guarantee
We're here to help
Our team of field application specialists are trained in immunoassay workflows. We can help you choose an assay or configure a panel, optimize an experiment, troubleshoot and more – face to face or on the phone at no cost.
Contact a field application specialistFor Research Use Only. Not for use in diagnostic procedures.
