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Maximizing performance in your bioreactor processes

Traditional feeding supplements require a compromise between working volume and feeding space in your bioreactor. Not anymore. Now you can overcome the conventional solubility limitations of fed-batch cell culture and achieve superior performance while minimizing risk.

Gibco EfficientFeed A+, B+, and C+ feeding supplements deliver super-concentrated nutrients in a complete, easy-to-prepare, dry-format feed, or ready to use super-concentrated liquid. Both options help maximize performance without compromise.

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Superior performance and flexibility

  • Achieve increases of at least 2- to 5-fold over batch culture, with a means to reach higher titers using super-concentration flexibility
  • Save time with Gibco AGT dry format for simple, rapid preparation that also helps reduce risk
  • Power to super-concentrate AGT dry format up to 150–200g/L at neutral pH, without any additional pH adjustment

Simplicity in use

  • Fewer closed-system connections needed
  • Comparable performance between liquid and AGT formats making it easier to scale-up

Ultimate flexibility through super-concentration

The EfficientFeed + Supplements are designed with the flexibility to super-concentrate chemically defined components up to 3X (i.e., 200 g/L) of original levels while remaining pH-neutral. This is accomplished with nutrient delivery technology that allows reconstitution without pH adjustments (i.e., just add water). By adding different amounts of water during reconstitution, you can concentrate these supplements 2X, or up to 3X.

How to super-concentrate:

  1. Weigh out the increased amount indicated here instead of the 1X amount.
  2. Mix for the extended time shown here to help completely dissolve the more concentrated supplements.
Product Concentration Dissolution Time
EfficientFeed A+ 1X (66.4 g/L) 20 min
  3X (199.2 g/L) 75 min
EfficientFeed B+ 1X (52.7 g/L) 20 min
  3X (158.1 g/L) 50 min
EfficientFeed C+ 1X (81.2 g/L) 40 min
  2X (162.4 g/L) 60 min

No more compromise

Until now, traditional fed-batch bioreactor space necessitated a compromise: the amount of complete feeding supplements one could add meant a trade-off between initial working volume and feeding space (dilution factor).

Today, EfficientFeed + Supplements empower you to deliver up to 200 g/L of needed nutrients in a complete, super-concentrated, pH-neutral feed. The reduction in required feeding space provides you with three ways to optimize your bioreactor output for every run:

  1. Reduce product dilution
  2. Maximize working volume
  3. Increase feeding of nutrients for increased titer

Feeding examples (traditional bolus feeding):

Product Concentration % Addition on Culture Days Indicated
  4 5 6 7 8 9 10
EfficientFeed™ A+ 1X (66.4 g/L) 10% - 10% - 10% - 10%
3X (199.2 g/L) 3.3% - 3.3% - 3.3% - 3.3%
EfficientFeed™ B+ 1X (52.7 g/L) 10% - 10% - 10% - 10%
3X (158.1 g/L) 3.3% - 3.3% - 3.3% - 3.3%
EfficientFeed™ C+ 1X (81.2 g/L) 10% - 10% - 10% - 10%
2X (162.4 g/L) 5% - 5% - 5% - 5%

Increase your bioprocess productivity

Gibco EfficientFeed + Supplements are designed to deliver the same 2- to 5-fold increases in titer possible with original EfficientFeed supplements, plus the flexibility to be super-concentrated up to 3X (or 200 g/L) of original levels while remaining pH-neutral and chemically defined.

In Figure 1, we demonstrate an improvement in titer with super-concentrated EfficientFeed C+ supplement.

Figure 1. Titer comparison with Gibco CD FortiCHO medium and glucose-only, 30% 2X EfficientFeed C (EFC, original formulation), and 15% EfficientFeed C+ supplement (EFC+) respectively.

We made EfficientFeed Supplements even better

Gibco EfficientFeed+ Supplements represent an improved version of earlier formulations. The result is improved titers relative to supplements from other vendors.

Figure 2 illustrates how titer comparisons for cell cultures and demonstrate how well EfficientFeed+ Supplements perform. A customer completed a head-to-head comparison in fed-batch culture of a DG44 cell line expressing IgG and demonstrated superior titers over approximately 20 days (peak titers at 80% harvest viability). In that comparison, EfficientFeed + Supplements demonstrated performance up to 139% better than those from other suppliers.

Figure 2. Titer comparison with CD FortiCHO medium and EfficientFeed C+ and other commercially available feeds on the market. EfficientFeed+ Supplements work better than the original versions. 

Reliable product quality

Figure 3 shows how product quality profiles remain consistent whether increasing feeding percentage or when switching from the original Efficientfeed formulation to the EfficientFeed+ Supplements. These improved formulations allow you to increase your bioreactor’s working volume. Feed more and achieve higher titers without sacrificing product quality.

Figure 3. Relative concentration of product quality profiles stays consistent when increasing feeding percentage or when switching from original EfficientFeed to EfficientFeed+ products.  

Gibco Advanced Granulation Technology (AGT) or liquid formats support process scale-up

AGT and liquid formats for EfficientFeed+ Supplements showed consistent performance in growth and titer. This makes it easy to switch between the two formats as you scale-up your process.


Figures 4 and 5
demonstrate that AGT and liquid formats have comparable performance.

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Figure 4. EfficientFeed+ concentrate supplement with CHO-S average growth

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Figure 5.  EfficientFeed+ concentrate supplement with CHO-S average titer

Simple preparation helps reduce risk

Our AGT dry media format provides ease-of-use while helping you reduce risk by minimizing the numerous steps and adjustments previously required to prepare conventional media.

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Storage of reconstituted liquid also reduces risk

Reconstituted EfficientFeed+ Supplements can be stored at room temperature (in the dark) for the period of a typical fed-batch bioreactor run. Making just one closed-system connection per bioreactor run reduces the opportunities for an error that could lead to contamination.

Bioprocessing resources

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