Maximizing performance in your bioreactor processes
Traditional feeding supplements require a compromise between working volume and feeding space in your bioreactor. Not anymore. Now you can overcome the conventional solubility limitations of fed-batch cell culture and achieve superior performance while minimizing risk.
Gibco EfficientFeed A+, B+, and C+ feeding supplements deliver super-concentrated nutrients in a complete, easy-to-prepare, dry-format feed, or ready to use super-concentrated liquid. Both options help maximize performance without compromise.
Superior performance and flexibility
- Achieve increases of at least 2- to 5-fold over batch culture, with a means to reach higher titers using super-concentration flexibility
- Save time with Gibco AGT dry format for simple, rapid preparation that also helps reduce risk
- Power to super-concentrate AGT dry format up to 150–200g/L at neutral pH, without any additional pH adjustment
Simplicity in use
- Fewer closed-system connections needed
- Comparable performance between liquid and AGT formats making it easier to scale-up
Ultimate flexibility through super-concentration
The EfficientFeed + Supplements are designed with the flexibility to super-concentrate chemically defined components up to 3X (i.e., 200 g/L) of original levels while remaining pH-neutral. This is accomplished with nutrient delivery technology that allows reconstitution without pH adjustments (i.e., just add water). By adding different amounts of water during reconstitution, you can concentrate these supplements 2X, or up to 3X.
How to super-concentrate:
- Weigh out the increased amount indicated here instead of the 1X amount.
- Mix for the extended time shown here to help completely dissolve the more concentrated supplements.
|EfficientFeed A+||1X (66.4 g/L)||20 min|
|3X (199.2 g/L)||75 min|
|EfficientFeed B+||1X (52.7 g/L)||20 min|
|3X (158.1 g/L)||50 min|
|EfficientFeed C+||1X (81.2 g/L)||40 min|
|2X (162.4 g/L)||60 min|
No more compromise
Until now, traditional fed-batch bioreactor space necessitated a compromise: the amount of complete feeding supplements one could add meant a trade-off between initial working volume and feeding space (dilution factor).
Today, EfficientFeed + Supplements empower you to deliver up to 200 g/L of needed nutrients in a complete, super-concentrated, pH-neutral feed. The reduction in required feeding space provides you with three ways to optimize your bioreactor output for every run:
- Reduce product dilution
- Maximize working volume
- Increase feeding of nutrients for increased titer
Feeding examples (traditional bolus feeding):
|Product||Concentration||% Addition on Culture Days Indicated|
|EfficientFeed™ A+||1X (66.4 g/L)||10%||-||10%||-||10%||-||10%|
|3X (199.2 g/L)||3.3%||-||3.3%||-||3.3%||-||3.3%|
|EfficientFeed™ B+||1X (52.7 g/L)||10%||-||10%||-||10%||-||10%|
|3X (158.1 g/L)||3.3%||-||3.3%||-||3.3%||-||3.3%|
|EfficientFeed™ C+||1X (81.2 g/L)||10%||-||10%||-||10%||-||10%|
|2X (162.4 g/L)||5%||-||5%||-||5%||-||5%|
Increase your bioprocess productivity
Gibco EfficientFeed + Supplements are designed to deliver the same 2- to 5-fold increases in titer possible with original EfficientFeed supplements, plus the flexibility to be super-concentrated up to 3X (or 200 g/L) of original levels while remaining pH-neutral and chemically defined.
In Figure 1, we demonstrate an improvement in titer with super-concentrated EfficientFeed C+ supplement.
We made EfficientFeed Supplements even better
Gibco EfficientFeed+ Supplements represent an improved version of earlier formulations. The result is improved titers relative to supplements from other vendors.
Figure 2 illustrates how titer comparisons for cell cultures and demonstrate how well EfficientFeed+ Supplements perform. A customer completed a head-to-head comparison in fed-batch culture of a DG44 cell line expressing IgG and demonstrated superior titers over approximately 20 days (peak titers at 80% harvest viability). In that comparison, EfficientFeed + Supplements demonstrated performance up to 139% better than those from other suppliers.
Reliable product quality
Figure 3 shows how product quality profiles remain consistent whether increasing feeding percentage or when switching from the original Efficientfeed formulation to the EfficientFeed+ Supplements. These improved formulations allow you to increase your bioreactor’s working volume. Feed more and achieve higher titers without sacrificing product quality.
Gibco Advanced Granulation Technology (AGT) or liquid formats support process scale-up
AGT and liquid formats for EfficientFeed+ Supplements showed consistent performance in growth and titer. This makes it easy to switch between the two formats as you scale-up your process.
Figures 4 and 5 demonstrate that AGT and liquid formats have comparable performance.
Figure 4. EfficientFeed+ concentrate supplement with CHO-S average growth
Figure 5. EfficientFeed+ concentrate supplement with CHO-S average titer
Simple preparation helps reduce risk
Our AGT dry media format provides ease-of-use while helping you reduce risk by minimizing the numerous steps and adjustments previously required to prepare conventional media.
Storage of reconstituted liquid also reduces risk
Reconstituted EfficientFeed+ Supplements can be stored at room temperature (in the dark) for the period of a typical fed-batch bioreactor run. Making just one closed-system connection per bioreactor run reduces the opportunities for an error that could lead to contamination.
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