Learn about basic properties of light, fluorophore excitation, emission, and Stokes shift.
Understand advantages of fluorescence microscopy compared to brightfield, the differences between magnification and resolution, and how using fluorescence can help increase resolution.
Learn about the light path in typical epifluorescence microscopes and their limits of resolution
Understand what the code on your objective means, why numerical aperture matters, and ways to make sure your microscope configuration will work for your experiment
Now you can take the Molecular Probes School of Fluorescence Basics of Imaging: Chapter 1—Fundamentals of fluorescence microscopy with you on your devices.
For Research Use Only. Not for use in diagnostic procedures.