Dynabeads for human T Cell expansion

Ready-to-use Dynabeads will allow you to optimize ex vivo T cell activation and proliferation in translational research, while preserving T cell viability and optimal immune function. Simultaneous signaling to TCR/CD3 and CD28 triggers a physiological activation and expansion of human T cells. You can also stimulate and expand human antigen-specific T cell lines or clones using Dynabeads Human T-Activator CD3/CD28/CD137.

This simple, efficient and consistent technology out-performs and replaces traditional home-brew methods for generic activation based on antigen-presenting cells (APCs), mitogens (e.g. ConA, PHA), soluble/plate-bound antibodies, or chemical activators, and is well documented in published literature. Expansion and re-infusion of genetically engineered T cells has the potential to revolutionize the treatment of leukemia and related blood cancers. A clinical research grade version is also available, allowing you to move from mouse studies to clinical research using the same technology platform.

Selection guide for human T cell activation and expansion

 Activation and expansion of polyclonal T cellsActivation and expansion of regulatory T cellsExpansion of antigen-specific T cellsIsolation and expansion of polyclonal T cellscGMP-grade beads for isolation and expansion of polyclonal T cells
 Dynabead Human
T-Activator CD3/CD28
Dynabeads Human Treg Expander Dynabeads Human T-Activator CD3/CD28/CD137
Dynabeads Human T-Expander CD3/CD28 Dynabeads CD3/CD28 CTS
Research gradeResearch gradeResearch gradeResearch gradeResearch-grade version of the cGMP-grade productFor research use or non-commercial manufacturing of cell-based products for clinical research
Starting samplesPure T cells isolated from any samplePure regulatory T cells isolated from any sampleT cell lines or T cell clonesPure T cells isolated from any sample, preferably from cryopreserved human PBMCCryopreserved human PBMC obtained from leukapheresis product
No. of cells processed4 x 107 cells /
mL beads
5 x 106 cells / mL beads5 x 106 cells / mL beads3.3 x 107 cells / mL beads1.6 x 108 cells / mL beads
Bead:T cell ratio for activation/expansion1:14:11:103:13:1
ApplicationsShort-term activation or expansion of polyclonal T cells without the need for feeder cells, e.g., for use in transfection/ transduction, T cell receptor signaling, proteomicsShort-term activation or expansion of pre-isolated regulatory
T cells without the need for feeder cells, e.g., for use in flow cytometry, MLR, proteomics, gene expression
Expansion of antigen-specific
T cells without the use of feeder cells, e.g., for use in flow cytometry, phenotyping, adoptive transfer
Isolation and expansion of CD3+
T cells, or expansion of pre-isolated T cells or T cell subsets without the need for feeder cells
Isolation and expansion of CD3+
T cells or expansion of pre-isolated T cells or T cell subsets without the need for feeder cells
Shelf life from production date2 years2 years2 years2 years2 years
Required materialsMagnets: magnets+ rIL-2 (for expansion)Magnets: magnets+ rIL-2 (for expansion)Magnets: magnets
+ rIL-2
Magnets: DynaMag CTS Magnet (Cat. No. 12102),
or visit magnets
+ rIL-2
Magnets: DynaMag CTS Magnet (Cat. No. 12102), or visit magnets+ rIL-2


The references listed below are among the many published papers documenting the performance of the Dynabeads technology for T cell activation and expansion:

  1. Eyquem, J et al. (2017) Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection. Nature 543: 113-117
  2. Mock, U et al. (2016) Automated manufacturing of chimeric antigen receptor T cells for adoptive immunotherapy using CliniMACS prodigy. Cytotherapy 18: 1002-1011
  3. Suarez, ER et al. (2016) Chimeric antigen receptor T cells secreting anti-PD-L1 antibodies more effectively regress renal cell carcinoma in a humanized mouse model. Oncotarget 7: 34341-34355
  4. Jacomet, F et al. (2015) Evidence for eomesodermin‐expressing innate‐like CD8+ KIR/NKG2A+ T cells in human adults and cord blood samples. Eur J Immunol.  45: 1926-33
  5. Kane, M et al. (2013) MX2 is an interferon-induced inhibitor of HIV-1 infection. Nature 502: 563-566
  6. Levring, TB et al. (2012) Activated human CD4+ T cells express transporters for both cysteine and cystine. Sci Rep. 2: 266
  7. Porter, DL et al. (2011) Chimeric Antigen Receptor–Modified T Cells in Chronic Lymphoid Leukemia. N Engl J Med 365:725-733.
  8. Kalos M et al (2011) T Cells with Chimeric Antigen Receptors Have Potent Antitumor Effects and Can Establish Memory in Patients with Advanced Leukemia. Sci Transl Med 3:95ra73 
  9. Bonyhadi M et al (2005) In vitro engagement of CD3 and CD28 corrects T cell defects in chronic lymphocytic leukemia. J Immunol 174:2366–2375.
  10. Rapoport AP et al (2005) Restoration of immunity in lymphopenic individuals with cancer by vaccination and adoptive T-cell transfer. Nat Med 11:1162–1163.
  11. Godfrey WR et al (2004) In vitro expanded human CD4+CD25+ T regulatory cells can markedly inhibit allogeneic dendritic cell stimulated MLR cultures. Blood 104:453–461.
  12. Coito S et al. (2004) Retrovirus-mediated gene transfer in human primary T lymphocytes induces an activation-and transduction/selection-dependent TCRBV repertoire skewing of gene-modified cells. Stem Cells Dev 13:71–81.
  13. Berger C et al (2003) CD28 costimulation and immunoaffinity-based selection efficiently generate primary gene-modified T cells for adoptive immunotherapy. Blood 101:476–484.
  14. Levine BL et al. (2002) Adoptive transfer of costimulated CD4+ T cells induces expansion of peripheral T cells and decreases CCR5 expression in HIV infection. Nat Med 8:47–53.

For Research Use Only. Not for use in diagnostic procedures.

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