We have categorized this list of publications citing the use of the PrimeFlow RNA Assay according to the application areas listed below. You may use any term to search the publications list by entering a search term and clicking Search.

  • Probe mRNA when an antibody to the protein target is unavailable
  • Analyze mRNA expression at the single-cell level
  • Compare RNA and protein kinetics in the same cell
  • Detect microRNA (miRNA)
  • Evaluate viral RNA in infected cells
  • Validate single-cell RNA sequencing results using PrimeFlow assay

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ApplicationTitleYear
Analyze mRNA expression at the single-cell level1,25-Dihydroxyvitamin D3 Restrains CD4+ T Cell Priming Ability of CD11c+ Dendritic Cells by Upregulating Expression of CD312019
Analyze RNA and protein kinetics in the same cellApplication of Imaging Flow Cytometry for the Characterization of Intracellular Attributes in Chinese Hamster Ovary Cell Lines at the Single Cell Level2019
Analyze mRNA expression at the single-cell levelVirally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)2019
Analyze mRNA expression at the single-cell levelMitochondrial mass governs the extent of T cell senescence2019
Analyze mRNA expression at the single-cell levelHuman lymphoid organ cDC2 and macrophages play complementary roles in T follicular helper responses2019
Analyze mRNA expression at the single-cell levelRegulation of membrane phospholipid asymmetry by Notch-mediated flippase expression controls the number of intraepithelial TCRαβ+CD8αα+ T cells2019
Analyze mRNA expression at the single-cell levelSIV infection modulates CD94 (KLRD1)+ NK cells in rhesus macaques2019
Analyze mRNA expression at the single-cell levelThe zinc transporter Zip14 (SLC39a14) affects Beta-cell Function: Proteomics, Gene expression, and Insulin secretion studies in INS-1E cells2019
Evaluate viral RNA in infected cellsIdentification of HIV transmitting CD11c+ human epidermal dendritic cells2019
Analyze mRNA expression at the single-cell levelNeutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors2019
Analyze mRNA expression at the single-cell levelMetabolic requirements of human pro-inflammatory B cells in aging and obesity2019
Analyze mRNA expression at the single-cell levelAltered differentiation is central to HIV-specific
CD4+ T cell dysfunction in progressive disease
2019
Evaluate viral RNA in infected cellsIn vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay2019
Analyze mRNA expression at the single-cell levelBhlhe40 and Bhlhe41 transcription factors regulate alveolar macrophage self‐renewal and identity2019
Evaluate viral RNA in infected cellsLatency reversal agents affect differently the latent reservoir present in distinct CD4+ T subpopulations2019
Analyze RNA and protein kinetics in the same cellActivation Receptor–Dependent IFN-γ Production by NK Cells Is Controlled by Transcription, Translation, and the Proteasome2019
Analyze mRNA expression at the single-cell levelThe Transcription Factor Bhlhe40 Programs Mitochondrial Regulation of Resident CD8+ T Cell Fitness and Functionality2019
Analyze mRNA expression at the single-cell levelFramework engineering to produce dominant T cell receptors with enhanced antigen-specific function2019
Analyze mRNA expression at the single-cell levelAdenosine mediates functional and metabolic suppression of peripheral and tumor-infiltrating CD8+ T cells2019
Analyze mRNA expression at the single-cell levelConversion of antigen-specific effector/memory T cells into Foxp3-expressing Treg cells by inhibition of CDK8/192019
Analyze mRNA expression at the single-cell levelCancer-Cell-Intrinsic cGAS Expression Mediates Tumor Immunogenicity2019
Analyze mRNA expression at the single-cell levelRORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients2019
Evaluate viral RNA in infected cellsDifferential and convergent utilization of autophagy components by positive-strand RNA viruses2019
Analyze mRNA expression at the single-cell levelSingle cell analyses reveal specific distribution of anti‐bacterial molecule Perforin‐2 in human skin and its modulation by wounding and Staphylococcus aureus infection2019
Analyze RNA and protein kinetics in the same cellA Multiparameter Flow Cytometry Analysis Panel to Assess CD163 mRNA and Protein in Monocyte and Macrophage Populations in Hyperinflammatory Diseases2019
Analyze mRNA expression at the single-cell levelActivity-by-Contact model of enhancer specificity from thousands of CRISPR perturbations2019
Analyze mRNA expression at the single-cell levelDiverse Immunomodulatory Effects of Individual IFNα Subtypes on Virus-Specific Cd8+ T Cell Responses2019
Analyze mRNA expression at the single-cell levelEndothelial toll‐like receptor 4 maintains lung integrity via epigenetic suppression of p16INK4a2019
Analyze RNA and protein kinetics in the same cellCCR7 Chemokine Receptor-Inducible lnc-Dpf3 Restrains Dendritic Cell Migration by Inhibiting HIF-1α-Mediated Glycolysis2019
Analyze mRNA expression at the single-cell levelMixed chimerism established by hematopoietic stem cell transplantation is maintained by host and donor T regulatory cells2019
Analyze mRNA expression at the single-cell levelGNAI1 and GNAI3 Reduce Colitis-Associated Tumorigenesis in Mice by Blocking IL6 Signaling and Downregulating Expression of GNAI22019
Detect microRNA (miRNA)MicroRNA-155 expression suggests a sex disparity in innate lymphoid cells at the single-cell level2019
Detect microRNA (miRNA)miR-181a/b-1 controls thymic selection of Treg cells and tunes their suppressive capacity2019
Analyze mRNA expression at the single-cell levelDiscovering metabolic disease gene interactions by correlated effects on cellular morphology2019
Analyze mRNA expression at the single-cell levelCross-Talk between Myeloid-Derived Suppressor Cells and Mast Cells Mediates Tumor-Specific Immunosuppression in Prostate Cancer2018
Analyze RNA and protein kinetics in the same cellProstaglandin D2 amplifies lupus disease through basophil accumulation in lymphoid organs2018
Analyze RNA and protein kinetics in the same cellType-I interferons inhibit interleukin-10 signaling and favor type 1 diabetes development in NOD mice2018
Analyze RNA and protein kinetics in the same cellRegulatory T cells control endothelial chemokine production and migration of T cells into intestinal tumors of APCmin/+ mice2018
Analyze RNA and protein kinetics in the same cellType-2 CD8+ T lymphocytes responsive to PGD2 and LTE4 in severe eosinophilic asthma2018
Analyze mRNA expression at the single-cell levelSequences enriched in Alu repeats drive nuclear localization of long RNAs in human cells2018
Analyze mRNA expression at the single-cell levelSecretion of autoimmune antibodies in the human subcutaneous adipose tissue2018
Evaluate viral RNA in infected cellsAttenuation of Simian Varicella Virus Infection by Enhanced Green Fluorescent Protein in Rhesus Macaques2018
Analyze mRNA expression at the single-cell levelTracking KLRC2 (NKG2C)+ memory-like NK cells in SIV+ and rhCMV+ rhesus macaques2018
Analyze RNA and protein kinetics in the same cellProspectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration2018
Evaluate viral RNA in infected cellsTools for Visualizing HIV in Cure Research2018
Analyze mRNA expression at the single-cell levelSingle-cell mRNA profiling reveals heterogeneous combinatorial expression of Hoxd genes during limb development2018
Evaluate viral RNA in infected cellsUninfected Bystander Cells Impact the Measurement of HIV-Specific Antibody-Dependent Cellular Cytotoxicity Responses2018
Analyze RNA and protein kinetics in the same cellMicroRNA networks associated with active systemic juvenile idiopathic arthritis regulate CD163 expression and anti‐inflammatory functions in macrophages through two distinct mechanisms2018
Analyze RNA and protein kinetics in the same cellNK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control2018
Evaluate viral RNA in infected cellsThe RNA surveillance proteins UPF1, UPF2 and SMG6 affect HIV-1 reactivation at a post-transcriptional level2018
Analyze mRNA expression at the single-cell levelFatty acid metabolism complements glycolysis in the selective regulatory T cell expansion during tumor growth2018
Analyze RNA and protein kinetics in the same cellRNA Flow Cytometry Using the Branched DNA Technique2018
Analyze RNA and protein kinetics in the same cellTCRβ combinatorial immunoreceptor expression by neutrophils correlates with parasite burden and enhanced phagocytosis during a Plasmodium berghei ANKA malaria infection2018
Analyze mRNA expression at the single-cell levelT cell cytolytic capacity is independent of initial stimulation strength2018
Analyze RNA and protein kinetics in the same cellProgressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract2018
Evaluate viral RNA in infected cellsInfection of epididymal epithelial cells and leukocytes drives seminal shedding of Zika virus in a mouse model2018
Analyze mRNA expression at the single-cell levelThe Transcription Factor ZEB2 Is Required to Maintain the Tissue-Specific Identities of Macrophages2018
Evaluate viral RNA in infected cellsImmature particles and capsid-free viral RNA produced by Yellow fever virus-infected cells stimulate plasmacytoid dendritic cells to secrete interferons2018
Detect microRNA (miRNA)MicroRNA Dysregulation in Lung Injury: The Role of the miR-26a/EphA2 Axis in 2 Regulation of Endothelial Permeability2018
Analyze RNA and protein kinetics in the same cellTCRβ-expressing macrophages induced by a pathogenic murine malaria correlate with parasite burden and enhanced phagocytic activity2018
Analyze mRNA expression at the single-cell levelSequestration of T cells in bone marrow in the setting of glioblastoma and other intracranial tumors2018
Analyze RNA and protein kinetics in the same cell; Detect microRNA (miRNA)Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis2018
Analyze mRNA expression at the single-cell levelHeterogeneous combinatorial expression of Hoxd genes in single cells during limb development2018
Analyze mRNA expression at the single-cell levelThe Long Non-coding RNA Flatr Anticipates Foxp3 Expression in Regulatory T Cells2018
Analyze mRNA expression at the single-cell levelVisualization of HIV-1 RNA Transcription from Integrated HIV-1 DNA in Reactivated Latently Infected Cells2018
Analyze RNA and protein kinetics in the same cellIL-6 Promotes T Cell Proliferation and Expansion under Inflammatory Conditions in Association with Low-Level RORγt Expression2018
Detect microRNA (miRNA)Detection and Differentiation of Multiple Viral RNAs Using Branched DNA FISH Coupled to Confocal Microscopy and Flow Cytometry 2018
Analyze mRNA expression at the single-cell levelGene expression variability across cells and species shapes innate immunity2018
Analyze RNA and protein kinetics in the same cellHVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage2018
Analyze mRNA expression at the single-cell levelCell-intrinsic Wnt4 controls early cDC1 commitment and suppresses development of pathogen-specific Type 2 immunity2018
Evaluate viral RNA in infected cellsFrequency of bovine viral diarrhea virus detected in subpopulations of peripheral blood mononuclear cells in persistently infected animals and health outcome2018
Analyze RNA and protein kinetics in the same cellCD19-positive antibody-secreting cells provide immune memory2018
Detect microRNA (miRNA)Functional pathways regulated by microRNA networks in CD8 T‐cell aging2018
Analyze RNA and protein kinetics in the same cellCommensal-specific T cell plasticity promotes rapid tissue adaptation to injury2018
Analyze mRNA expression levels when antibody is limitedThe prostaglandin D2 receptor CRTH2 regulates accumulation of group 2 innate lymphoid cells in the inflamed lung2015
Analyze mRNA expression levels when antibody is limitedPhenotypic and functional characterization of the major lymphocyte populations in the fruit-eating bat Pteropus alecto2016
Analyze mRNA expression levels when antibody is limitedA Leukocyte Immune-Type Receptor Subset Is a Marker of Antiviral Cytotoxic Cells in Channel Catfish, Ictaluruspunctatus2016
Analyze mRNA expression at the single-cell levelSingle-Cell Genomics Unveils Critical Regulators of Th17 Cell Pathogenicity2015
Analyze mRNA expression at the single-cell levelA Model System for the Study of Gene Expression in the Undergraduate Laboratory2016
Analyze mRNA expression at the single-cell levelAnalysis of EZH2 expression in regulatory T-cells2017
Analyze mRNA expression at the single-cell levelHLA-DR expression variations on human regulatory T cells is associated with impaired immune tolerance2017
Analyze mRNA expression at the single-cell levelCancer‐related mRNA expression analysis using a novel flow cytometry‐based assay2017
Analyze mRNA expression at the single-cell levelDifferential in vivo activation of monocyte subsets during low-grade inflammation through experimental endotoxemiain humans 2016
Analyze mRNA expression at the single-cell levelSex differences in plasmacytoiddendritic cell levels of IRF5 drive higher IFN-α production in women2015
Analyze mRNA expression at the single-cell levelImmunosuppressive plasma cells impede T-cell-dependent immunogenic chemotherapy2015
Analyze mRNA expression at the single-cell levelDectin-1 activation unlocks IL12A expression and reveals the TH1 potency of neonatal dendritic cells2015
Analyze mRNA expression at the single-cell levelFANCA knockout in human embryonic stem cells causes a severe growth disadvantage2014
Compare RNA and protein kinetics in the same cellMeasurement of Low-Abundance Intracellular mRNA Using Amplified FISH Staining and Image-Based Flow Cytometry2016
Compare RNA and protein kinetics in the same cellAryl Hydrocarbon Receptor Activation Suppresses EBF1 and PAX5 and Impairs Human B Lymphopoiesis2017
Compare RNA and protein kinetics in the same cellConsumption of a High-Fat, High-Calorie Meal is Associated with an Increase in Intracellular Co-localization of PPAR-γ mRNA and Protein in Monocytes2017
Compare RNA and protein kinetics in the same cellAffinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells2016
Compare RNA and protein kinetics in the same cellSimultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins2016
Compare RNA and protein kinetics in the same cellUsing Image-Based Flow Cytometry with FISH-Based Flow RNA Assay to Simultaneously Detect Intracellular TNF-α Protein and mRNA in Monocytes Following LPS Stimulation2015
Compare RNA and protein kinetics in the same cellMeasurements of CD34+/CD45-dim stem cells predict healing of diabetic neuropathic wounds2016
Compare RNA and protein kinetics in the same cellHuman mast cells are major IL-22 producers in patients with psoriasis and atopic dermatitis2015
Compare RNA and protein kinetics in the same cellUnipotent megakaryopoietic pathway bridging hematopoietic stem cells and mature megakaryocytes2015
Compare RNA and protein kinetics in the same cellB10 cell Frequencies and suppressive capacity in Myasthenia gravis are associated with Disease severity2017
Compare RNA and protein kinetics in the same cellPhenotypic and functional characteristics of HLA-DR+ neutrophils in Brazilians with cutaneous leishmaniasis2017
Compare RNA and protein kinetics in the same cellGerminal center B cell development has distinctly regulated stages completed by disengagement from T cell help2017
Compare RNA and protein kinetics in the same cellHuman lung tumor FOXP3+ Tregs upregulate four “Treg-locking” transcription factors2017
Detect microRNA (miRNA)MicroRNA-302d targets IRF9 to regulate the IFN-induced gene expression in SLE2017
Detect microRNA (miRNA)High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry2014
Evaluate viral RNA in infected cellsKinetics of HIV-1 latency reversal quantified on the single cell level using a novel flow-based technique2016
Evaluate viral RNA in infected cellsSingle-cell characterization of viral translation-competent reservoirs in HIV-infected individuals2016
Evaluate viral RNA in infected cellsA Novel Single-Cell FISH-Flow Assay Identifies Effector Memory CD4+ T cells as a Major Niche for HIV-1 Transcription in HIV-Infected Patients2017
Evaluate viral RNA in infected cellsKinetics of HIV-1 Latency Reversal Quantified on the Single-Cell Level Using a Novel Flow-Based Technique2016
Evaluate viral RNA in infected cellsMultiparametric characterization of rare HIV-infected cells using an RNA-flow FISH technique2017
Evaluate viral RNA in infected cellsSingle-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome2017
Evaluate viral RNA in infected cellsImproved detection of bovine viral diarrhea virus in bovine lymphoid cell lines using PrimeFlow RNA assay2017
Evaluate viral RNA in infected cellsPersistent KSHV Infection Increases EBV-Associated Tumor Formation In Vivo via Enhanced EBV Lytic Gene Expression2017
Evaluate viral RNA in infected cellsUnderstanding the role of Epstein-Barr virus in T-and NK-cell disorders2016
Evaluate viral RNA in infected cellsThe Alphabet Soup of HIV Reservoir Markers2017
Evaluate viral RNA in infected cellsHighlights from the seventh international workshop on HIV persistence during therapy, 8–11 December 2015, Miami, Florida, USA2016
Evaluate viral RNA in infected cellsCurrent views on HIV-1 latency, persistence, and cure2017
Evaluate viral RNA in infected cellsRNA flow cytometric FISH for investigations into HIV immunology, vaccination and cure strategies2017
Evaluate viral RNA in infected cellsFISHing Out the Hidden Enemy: Advances in Detecting and Measuring Latent HIV-Infected Cells2017
Validate the single-cell RNA sequencing results by PrimeFlowBrain trauma elicits non-canonical macrophage activation states2016

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