AlgiMatrix 3D Culture System is the first user-friendly, animal-free bioscaffold available to develop higher fidelity cell culture models that better predict diseases and drug responses.
Algimatrix 3D cell culture scaffold is made from alginates, which are natural polymers often found in seaweed and other plants.
AlgiMatrix sponge is formulated using pharmaceutical-grade raw materials from brown seaweed. Its chemically defined, pure, non-toxic macro-porous structure delivers lot-to-lot consistency, superior cell loading, and excellent nutrient delivery without damage to cells. The AlgiMatrix sponge is biodegradable and stable at room temperature.
AlgiMatrix 3D cell culture scaffold is available pre-loaded in 96-, 24-, or 6-well plates that integrate into any workflow. Cells can be added directly into these sterile multiwell plates; no pre-treatment or other preparation is required.
The AlgiMatrix 3D Culture System is suitable for a broad range of procedures, including multicellular tumor spheroid assays (MCTS), hepatocyte and cardiomyocyte organogenesis studies, co-culture studies, high-throughput drug screening assays, and embryonic stem cell 3D differentiation. This broad applicability fulfills a critical need for cell-based screening, drug discovery, and in vitro human cell therapy research.
After spheroids have formed and the desired experimental parameters met (culture duration, inducers, etc.) perform assays as desired:
|Cell type||Source||Cell definition||Cell source|
|Hepatocytes: pure culture||Rat||Primary, adult||Isolation|
|Hepatocytes: co-culture with Kupffer cells or non-parenchymal cells||Rat||Primary, adult||Isolation|
|Mixed hepatoblasts and oval (liver stem) cells||Rat||Primary, neonatal||Isolation|
|Hepatocytes—C3A||Human||Cell line, clone derivative of HepG2||ATCC (CRL-10741)|
|Endothelial cells dermal microvascular (HDMEC)||Human||Primary, frozen||Clonetics (CC-2543)|
|Endothelial cells umbilical vein (HUVEC)||Human||Primary, fresh||Isolation|
|Endothelial cells dermal microvascular-transfected (HMEC-1)||Human||Cell line (transfected with encoding SV-40 large T antigen)||Gift*|
|Endothelial cells bovine aortic (BAEC)||Bovine||Primary, frozen||Isolation|
|Dermal fibroblasts (NHDF)||Human||Primary, frozen||Clonetics (CC-2511)|
|Cardiac myocytes||Rat||Primary, embryonic||Isolation|
|Cardiac myocytes||Rat||Primary, neonatal||Isolation|
|Embryonic stem cells||Human||(H-9-Wisconsin lines, Israeli lines)||Technion|
|Mesenchymal stem cells, bone marrow||Human||Primary, bone marrow||Isolation based on plate adherence|
|Mesenchymal stem cells, cord blood (CD133+ enriched)||Human||Primary, cord blood||Isolation based on plate adherence|
|Hematopoietic stem cells, cord blood (CD133+ enriched)||Human||Primary, cord blood||Isolation|
*Gift courtesy of Smadar Cohen and Tsiona Elkayam Ben-Gurion, University of Negev
For Research Use Only. Not for use in diagnostic procedures.