Conditions for using restriction endonucleases for traditional cloning to ensure successful restriction digestion, ligation and colony screening are explained here.
Learn about various cloning strategies, including PCR cloning, subcloning, genomic and cDNA library construction, and shotgun sequencing.
Learn common troubleshooting strategies for basic cloning methods including proper controls to evaluate ligation and transformation problems.
For Research Use Only. Not for use in diagnostic procedures.