KingFisher Flex System


The most versatile benchtop sample prep instrument in the lab for 24 or 96 samples per run. Consistent extraction and purification of DNA, RNA, Protein, and Cells.

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Product features

Typical run with KingFisher instrument

KingFisher Flex details

Instrument size Benchtop
Throughput level Medium to high
Processing volume range
  • PCR plate (20–100 μL*), skirted
  • 20–200 μL: 96-well plate
  • 50–1,000 μL: 96 deep-well plate
  • 200–5,000 μL: 24 deep-well plate
Samples per run 24 or 96
Customizable protocols Yes
Heating/cooling From 5°C above ambient temperature to 115°C
Ultraviolet lamp No

* Or similar skirted PCR plate.

How KingFisher instruments work

KingFisher instruments automate extraction of DNA, RNA, protein, and cells by moving magnetic beads (not liquids), leading to clean extractions and enabling consistent results. Using a simple process (bind, wash, elute), KingFisher instruments can automate the extraction of any analyte of interest with a bead on it.

Kits and reagents

Choose from a wide range of magnetic bead-based kits and reagents from trusted brands, such as Dynabeads and MagMAX.

Search now

Plates and plastics

  • Made of polypropylene
  • Low binding affinity for biomolecules, ideal for magnetic particle processing
  • Sterile plastics come with a purity certificate and are guaranteed to be free of RNase, DNase, DNA and endotoxin contamination, shelf life is 5 years

Select three different plate formats for a range of input volumes.

Software and protocols

Nucleic Acid Protocols

  • Protocols designed for nucleic acid purification procedures and reagent kits

Search and download automated protocols

IP/Protein Protocols

  • Protocols for Immunoprecipitation, Protein A, G, Sheep Anti-Mouse, Sheep Anti-Rabbit, Streptavidin

Search and download automated protocols
Learn more

BindIt Software

BindIt Software

  • Create and store protocols in a PC database
  • Compatible with all KingFisher instruments
  • Easy to use
  • Generate reports

Design or customize your own protocols

Service and support

Instrument and application specialists

Whether servicing your KingFisher purification system or addressing your workflow application questions, our more than 1,000 trained professionals make up the industry’s largest network, ready to assist you when you need it.

Quick-start training

The KingFisher system includes SmartStart orientation to get you up and running quickly in your lab. The orientation includes basic familiarization and setup with online instrument management and includes on-site training with standard kits.

Warranty and service plans

Every KingFisher system comes with a one-year warranty. Extended-coverage service plans are also available at the time of instrument purchase. Whether your laboratory requires the highest service levels and adherence to stringent regulatory guidelines, or you need to maximize performance with a limited budget—or anything in between—we’ll work with you to develop a solution that best suits your needs. KingFisher instrument services and support

Find out more at thermofisher.com/instrumentservices or contact us at thermofisher.com/contactus.

KingFisher Flex how-to videos

How to unbox the KingFisher Flex

Guiding you through the process to safely unbox your KingFisher Flex Instrument.

Remove transportation locks

After receiving your KingFisher Flex, this video will walk through the steps to remove the transportation locks.

Install tip shield

Walking through the process to install the tip shield on the KingFisher Flex.

Power on

What to know in order to power on the KingFisher Flex Instrument.

Change heat block

Helping identify the process to change the heat block on the KingFisher Flex instrument.

Install magnet head and check tip comb

Helping with the steps to install the magnet head of the KingFisher Flex and also how to check the tip comb before loading instrument.

Connect computer and use BindIt software

Walking through the process to connect the KingFisher Flex top laptop and installing the BindIt software.

Transfer BindIt software protocols

Update your instrument with your protocol, simple steps to transfer your BindIt protocol to the KingFisher Flex instrument.

Remove magnet head

Simple steps to help remove the magnet head safely from the KingFisher Flex instrument.

Product features

Typical run with KingFisher instrument

KingFisher Flex details

Instrument size Benchtop
Throughput level Medium to high
Processing volume range
  • PCR plate (20–100 μL*), skirted
  • 20–200 μL: 96-well plate
  • 50–1,000 μL: 96 deep-well plate
  • 200–5,000 μL: 24 deep-well plate
Samples per run 24 or 96
Customizable protocols Yes
Heating/cooling From 5°C above ambient temperature to 115°C
Ultraviolet lamp No

* Or similar skirted PCR plate.

How KingFisher instruments work

KingFisher instruments automate extraction of DNA, RNA, protein, and cells by moving magnetic beads (not liquids), leading to clean extractions and enabling consistent results. Using a simple process (bind, wash, elute), KingFisher instruments can automate the extraction of any analyte of interest with a bead on it.

Kits and reagents

Choose from a wide range of magnetic bead-based kits and reagents from trusted brands, such as Dynabeads and MagMAX.

Search now

Plates and plastics

  • Made of polypropylene
  • Low binding affinity for biomolecules, ideal for magnetic particle processing
  • Sterile plastics come with a purity certificate and are guaranteed to be free of RNase, DNase, DNA and endotoxin contamination, shelf life is 5 years

Select three different plate formats for a range of input volumes.

Software and protocols

Nucleic Acid Protocols

  • Protocols designed for nucleic acid purification procedures and reagent kits

Search and download automated protocols

IP/Protein Protocols

  • Protocols for Immunoprecipitation, Protein A, G, Sheep Anti-Mouse, Sheep Anti-Rabbit, Streptavidin

Search and download automated protocols
Learn more

BindIt Software

BindIt Software

  • Create and store protocols in a PC database
  • Compatible with all KingFisher instruments
  • Easy to use
  • Generate reports

Design or customize your own protocols

Service and support

Instrument and application specialists

Whether servicing your KingFisher purification system or addressing your workflow application questions, our more than 1,000 trained professionals make up the industry’s largest network, ready to assist you when you need it.

Quick-start training

The KingFisher system includes SmartStart orientation to get you up and running quickly in your lab. The orientation includes basic familiarization and setup with online instrument management and includes on-site training with standard kits.

Warranty and service plans

Every KingFisher system comes with a one-year warranty. Extended-coverage service plans are also available at the time of instrument purchase. Whether your laboratory requires the highest service levels and adherence to stringent regulatory guidelines, or you need to maximize performance with a limited budget—or anything in between—we’ll work with you to develop a solution that best suits your needs. KingFisher instrument services and support

Find out more at thermofisher.com/instrumentservices or contact us at thermofisher.com/contactus.

KingFisher Flex how-to videos

How to unbox the KingFisher Flex

Guiding you through the process to safely unbox your KingFisher Flex Instrument.

Remove transportation locks

After receiving your KingFisher Flex, this video will walk through the steps to remove the transportation locks.

Install tip shield

Walking through the process to install the tip shield on the KingFisher Flex.

Power on

What to know in order to power on the KingFisher Flex Instrument.

Change heat block

Helping identify the process to change the heat block on the KingFisher Flex instrument.

Install magnet head and check tip comb

Helping with the steps to install the magnet head of the KingFisher Flex and also how to check the tip comb before loading instrument.

Connect computer and use BindIt software

Walking through the process to connect the KingFisher Flex top laptop and installing the BindIt software.

Transfer BindIt software protocols

Update your instrument with your protocol, simple steps to transfer your BindIt protocol to the KingFisher Flex instrument.

Remove magnet head

Simple steps to help remove the magnet head safely from the KingFisher Flex instrument.