For additional performance data for specific kits and reagents, follow their Cat. No. links in the figure captions. For additional application data, download the application notes listed in this section and on the Resources page.

SARS-CoV-2 testing

Nasopharyngeal swab specimens were spiked with 250 copies/mL (left) or 750 copies/mL (right) of SARS-CoV-2 viral RNA (1x or 3x the limit of detection, respectively), and RNA was extracted from 200 μL or 400 μL of the spiked sample in triplicate using the MagMAX Viral/Pathogen Kit (Cat. No. A42352) with (+) or without (–) Wash 3. After elution in 50 μL, the samples were assayed by qRT-PCR for four RNA targets (colored symbols) in triplicate. Ct values for each target were highly consistent (symbols cluster closely) and virtually identical between sample volumes and wash conditions, validating the streamlined lower-volume protocols. All negative samples had Ct values of “Undetected” (data not shown).

Ct values for contrived SARS-CoV-2–positive nasopharyngeal swab samples with different sample preparation workflows.

Ct values for contrived SARS-CoV-2–positive nasopharyngeal swab samples

Recovery and purity of DNA from whole blood

A KingFisher Duo Prime was used to isolate DNA from two donors’ whole blood using the MagMAX Multi-Sample Ultra 2.0 Kit (Cat. No. A36570). Samples >400 µL were processed using the large-volume protocol in a 24-well plate, while samples 50–400 µL were processed using the small volume protocol in a 96-well plate. (No sample volume normalization was needed, even when 50 µL and 400 µL samples were run at the same time on the same plate.) Run time was 45 minutes. (Top) Recovery volumes were linear across both small and large volume inputs. (Bottom) A260/A280 and A260/A230 ratios demonstrated high purity across sample volumes.

Recovery and purity of DNA isolated from whole blood.

MagMax-workflow-22

Sequencing consistency of FFPE cancer samples

DNA and RNA were extracted from matched core needle biopsies and fine-needle aspirate samples using the MagMAX FFPE DNA/RNA Ultra Kit (Cat. No. A31881) on the KingFisher Duo prime. Libraries were prepared and samples were templated and sequenced. Sequencing metrics such as (A) mean read length and (B) DNA mapping and uniformity were consistent, demonstrating functional recovery of nucleic acids from different specimen types.

Sequencing of cancer research samples.

Sequencing of cancer research samples

miRNA biomarker screening for prostate cancer

Total RNA was isolated from the serum of 10 individuals with prostate cancer and 10 healthy individuals using the MagMAX mirVana Total RNA Isolation Kit (Cat. No. A27828) on the KingFisher Flex. Expression levels of 19 miRNAs were measured by qRT-PCR using the TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007) and TaqMan Advanced miRNA Assays (Cat. No. A25576). The y-axis displays ΔCt values for each miRNA, normalized to reference mRNA. Large differences between healthy male samples (blue) and prostate cancer samples (green) identify potential cancer biomarkers.

Sequencing of cancer research samples.

Sequencing of cancer research sample

Microbiota identification

Total nucleic acid from fecal, urine, and saliva samples from two donors was isolated using the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42357 and A42358) on the KingFisher Flex, submitted for shotgun metagenomic sequencing, and compared with known microbial profiles. The figure shows abundance heat maps for selected microbiota for all samples. Results confirmed that each body habitat harbored different dominant signature taxa, with only a few shared species, mostly between urine and saliva. In each community, there was substantial overlap between donors but also marked individual differences.

Microbiota heat maps for three samples from two donors.

Microbiota heat maps for three samples from two donors.

Veterinary pathogen detection

For a study at the University of California Davis School of Veterinary Medicine, whole blood, feces, and nasal secretions were obtained from canine, feline, and equine species. Total nucleic acids were extracted on the KingFisher Flex and a vacuum-based automated system and amplified with qPCR. Quantification cycle (Cq) values were comparable for the two methods and the total number of pathogens detected was similar. However, the extraction time with the KingFisher Flex was three times faster.

Detection of veterinary pathogens with qPCR.


Immunoprecipitation

Jurkat cells expressing CD81 were lysed and incubated with Dynabeads Protein G (Cat. No. 10004D) coated with an anti-CD81 antibody. CD81 was isolated from the cell lysate in triplicate using an optimized walkaway IP protocol on the KingFisher Duo Prime and Flex instruments and compared with the manual protocol. Electrophoresis and western blotting confirmed good performance on the two instruments, equivalent to the results obtained with the manual protocol.

Immunoprecipitation (IP) of CD81 using manual vs automated methods.

Immunoprecipitation (IP) of CD81 using manual vs automated methods.

Peptide mapping

The drug infliximab was digested using the SMART Digest Trypsin Kit on the KingFisher Duo Prime. Analysis of the UV peptide map by LC-MS confirmed complete sequence coverage of 100% for both the light and heavy chain of the antibody. In other data, the automated protocol was found to be reproducible and easy to use, even by those who had no prior experience with protein digestion.

Sequence coverage map of infliximab drug product.

Sequence coverage map of infliximab drug product.

Exosome pull-down

CD9+ exosomes from SW480 colon cancer cells were isolated on the KingFisher Flex by targeting CD9 followed by staining and analyzed by flow cytometry. CD9 exosomes from Jurkat cells were included as controls. Histograms show a distinct CD9+ population (red) among the SW480 cells (B) compared to Jurkat controls (C). The scatter plot and gating profile is also shown (A).

Flow cytometry of exosomes isolated with Dynabeads magnetic beads.

Flow cytometry of exosomes isolated with Dynabeads magnetic beads.

For Research Use Only. Not for use in diagnostic procedures.