Our newest multimode microplate reader combines simplicity with top-of-the-line performance. Designed for researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX Multimode Microplate Reader offers features that help save time and reduce common errors in the lab—including automated dynamic range selection, which adjusts the optimal reading range based on signal intensities; and built-in safety controls, which alert you to potential errors before they happen.

 


Features

In addition to the common features of all Microplate Reader instruments, the Varioskan LUX offers:

Automatic dynamic range selection

Designed to automatically select the optimal reading range based on the signal intensity in each well. This feature saves time and reduces the risk of loss of sensitivity or over-saturation of signals.

 

Integrated gas control

The ability to control O2 and CO2 levels in your microplate reader replaces the need for constant handling of plates between the incubator and reader, and helps to ensure that cells remain alive throughout the experiment.

Smart safety controls

Automatic safety checks designed to alert you of potential problems before they happen. This decreases the potential of a range of experimental errors, from user mistakes to power outages.

 

Flexible wavelength selection

The instrument selects the measurement wavelength using filters or monochromators, depending on which is optimal for each measurement technology.


Available measurement technology configurations

All configurations available with top or top and bottom fluorescence intensity reading; none, one, or two dispensers; and optional integrated gas modules.

 

System configuration options

Absorbance and Fluorescence intensity

Absorbance, Fluorescence intensity, and Luminescence

Absorbance, Fluorescence intensity, Luminescence, and AlphaScreen

Absorbance, Fluorescence intensity, Luminescence, and Time-resolved fluorescence 

Absorbance, Fluorescence intensity, Luminescence, Time-resolved fluorescence, and AlphaScreen


Videos and demos


Specifications

Category

Attribute

Description

Absorbance

Plate types

6-384 well plates

 

Wavelength selection

Double monochromators

 

Wavelength range

200–1,000 nm

 

Light source

Xenon flash lamp

 

Read out range

0–6 Abs

 

Linear measurement range

0–4 Abs (96-well plate) at 450 nm, ± 2%

0–3 Abs (384-well plate) at 450 nm, ± 2%

 

Accuracy

0.003 Abs or ± 2%, at 200–399 nm (0–2 Abs)

0.003 Abs or ± 1%, at 400–1,000 nm (0–3 Abs)

 

Precision

SD <0.001 Abs or CV <0.5%, at 450 nm (0–3 Abs)

Fluorescence Intensity

Plate types

6 – 1536-well plates (top reading)

6 – 384-well plates (bottom reading)

 

Wavelength selection

Double excitation and emission monochromators

 

Excitation wavelength range

200–1,000 nm

 

Emission wavelength range

270–840 nm

 

Light source

Xenon flash lamp

 

Sensitivity

Top reading: <0.4 fmol fluorescein/well (black 384-well plate)

Bottom reading: <4 fmol fluorescein/well, (clear bottom black 384 square well plate)

 

Dynamic range

Top reading >6 decades

Bottom reading >5.5 decades

Time-resolved fluorescence

Plate types

6–1,536 well plates

 

Wavelength selection

Filters (spectral scanning with double excitation and emission monochromators)

 

Excitation wavelength range

Fixed to 334 nm (spectral scanning 200–840 nm)

 

Emission wavelength range

400–700 nm (spectral scanning 270–840 nm)

 

Light source

Xenon flash lamp

 

Sensitivity

<1 amol Eu/well (white low volume 384 well plate)

 

Dynamic range

>6 decades

Luminescence

Plate types

6–1536 well plates (spectral scanning 6-384 well plates)

 

Wavelength selection

Direct or filters (spectral scanning with double monochromators)

 

Wavelength range

360–670 nm

 

Sensitivity

<7 amol ATP/well (white 384-well plate)

 

Dynamic range

>7 decades

Alpha

Plate types

6-1536 well plates

 

Wavelength selection

Filters

 

Excitation wavelength range

Fixed to 680 nm

 

Emission wavelength range

400–660 nm

 

Light source

LED

 

Sensitivity

<100 amol phosphotyrosine/well (white 384-well plate)

Dispensing

Plate types

6-384 well plates

 

No. of dispensers

None, one or two

 

Syringe size

1 ml (standard), 5 ml (optional)

 

Dispensing volume

2–5,000 μl, in 1 µl increments (1 ml syringe)

5–25,000 μl, in 5 µl increments (5 ml syringe)

 

Accuracy

<1 μl with 50 μl (0.4 mm tip)

<0.2 μl with 5 μl (0.25 mm tip)

 

Precision

<1 μl with 50 μl (0.4 mm tip)

<0.25 μl with 5 μl (0.25 mm tip)

 

Dead volume

Reagent loss <100 μl, Total tubing volume <800 μl

Incubator and Shaker

Temperature range

From ambient + 4°C to 45 °C

 

Shaking type

Orbital

Integrated Gas Module

Measurement chamber O2 concentration accuracy

±1.0% (37°C, 1% O2)

 

Measurement chamber CO2 concentration accuracy

±1.0%. (37°C, 5% CO2)

 

Measurement chamber O2 concentration

1-21%

 

Measurement chamber CO2 concentration

0.1-15%

  Gas concentration recovery time 10 minutes

General Features

Measurement modes

End-point, kinetic, spectra, multipoint and kinetic spectra

 

Measurement speed

Reads a 96-well plate in 15 s, a 384-well plate in

45 s, and a 1,536-well plate in 135 s (minimum times)

 

Interface

PC software (SkanIt software)

 

Dimensions (D x W x H)

58 x 53 x 51 cm (23 x 21 x 20 in)

 

Weight

53 - 60 kg, (117-132 lb), depending on configuration

For Research Use Only. Not for use in diagnostic procedures.