Below you will find tips for qPCR assay design as well as videos about best practices for real-time PCR assays, including how to measure PCR efficiency, baselines, and thresholds; the best quencher molecule; and the purpose of ROX dye in your assay.
Know your target but don’t want to do all the above work? Use one of our more than 28 million pre-designed TaqMan Assays. These assays are designed with a validated process and come with the TaqMan Guarantee.
Have a target not found using the pre-designed assay search tool? Use our Custom Assay Design Tool for custom assays designed using the same process as our pre-designed assays.
For Research Use Only. Not for use in diagnostic procedures.