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Applied Biosystems TaqMan Fast Virus 1-Step Master Mix is a one-tube, one-step, 4X master mix designed for reliable, high-sensitivity real-time RT-PCR even in the presence of common reaction inhibitors. Its features have been selected to enhance virus detection in the kinds of research samples labs are likely to encounter.
High sensitivity allows viral detection in samples with low levels of target, while buffer components are formulated to handle common RT-PCR inhibitors in research samples contaminated with blood, dirt, or tissues. The mix has been optimized to work with multiple target amplicons. A single-tube format allows for uniform handling and processing for both RNA and DNA viruses while two formulation options (with and without ROX dye) enable ultimate multiplexing flexibility.
Key features of TaqMan Fast Virus 1-Step Master Mixes are designed to boost virus detection:
High sensitivity
Higher-concentration master mixes allow more accurate quantification with low-titer samples or smaller reactions and fast cycling protocols with normal-titer samples.
Target flexibility
Single-run profile compatible with RNA and DNA and on any supported instrument allows for easy mix-and-match of targets on a plate.
Consistent results
Formulated to handle common RT-PCR inhibitors found in blood, stool, and other difficult research samples
Optimized for multiple targets
Excellent performance for both singleplex and multiplex reactions, with exogenous or endogenous internal controls. The formulation without ROX reference dye enables even greater flexibility for multiplexing of four targets in a single reaction well.
Increased qRT-PCR speed on both fast and standard instruments, speeding up your time to results and maximizing the usage of your real-time PCR instruments.
Because virus research often includes multiplexed primers and probes and internal reaction controls, we have optimized master mixes to work with multiple targets. TaqMan Fast Virus 1-Step master mixes demonstrate exceptional sensitivity in commonly used duplex reactions. For higher-order multiplexing, TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) has increased flexibility to accommodate four targets in a single reaction well. By removing the ROX passive reference dye, this formulation of the master mix enables customers to use other dyes, such as JUN™ dye, in the channel.
It is common for labs to test for both RNA and DNA viruses in a variety of samples. To simplify your experiments, a single TaqMan Fast Virus 1-Step Master Mix protocol has been developed to assay both types of nucleic acids, so you can perform RNA and DNA virus queries next to each other on the same plate using the same handling steps.
Research samples commonly assayed for viruses include blood, dirt, and tissues. Buffer components and proprietary additives in TaqMan Fast Virus 1-Step Master Mix have been optimized to handle RT-PCR inhibitors to help ensure consistent performance even with these difficult samples, so you can be more confident in your results.
For Research Use Only. Not for use in diagnostic procedures.