Custom and Custom Plus TaqMan™ Copy Number Assays enable the generation of specific, reproducible and easy-to-interpret copy number results. They are suited for validation studies and large sample screens and provide a flexible targeted approach to copy number analysis.
Each custom assay is a mix of forward primer, reverse primer, and FAM™ dye-labeled TaqMan™ MGB™ probe. Designed to run under universal oligo concentration and thermal cycling conditions, these assays are simple to use. Just add TaqMan™ Genotyping Master Mix and your sample to generate sensitive and reproducible data.
There are two custom assay options to meet your research needs (Custom TaqMan® Copy Number Assays and Custom Plus TaqMan® Copy Number Assays). Both allow you to submit a target sequence to our TaqMan® Assay design pipeline, which uses your input sequence to design custom primer and probe sequences. See the key differences between the two options in Table 1:
|Feature||Custom Plus TaqMan® Copy Number Assays||Custom TaqMan® Copy Number Assays||Predesigned TaqMan® Copy Number Assays|
|Designed with copy number-specific algorithm optimized for performance||✔||✔||✔|
|Available limited to human and mouse assays||✔||✔|
|Contains TaqMan® FAM™ dye-labeled MGB™ probes and 2 unlabeled PCR primers||✔||✔||✔|
|Targets undergo SNP and repetitive sequencing mask||✔||✔|
|Reference assay compatibility check||✔ (optional)||✔ (optional)||✔|
|Assay sequences provided||✔|
|Assay context sequences and genome location provided||✔||✔|
Browse the GeneAssist™ Copy Number Assay Workflow Builder. Navigate to the human or mouse genomic region of interest to select predesigned assays, or select a targe region for Custom Plus Assay design. Alternatively, submit pre-masked sequences for standard Custom Assay design or submit primer/probe pair sequences for assay synthesis.
|Catalog Number||Product Name||No. of 10 µL reactions (384-well plate)||No. of 20 µL reactions (96-well plate)|
|4442487||Custom Plus TaqMan® Copy Number Assay, SM||720||360|
|4442520||Custom Plus TaqMan® Copy Number Assay, MED||1,500||750|
|4442488||Custom Plus TaqMan® Copy Number Assay, LG||5,800||2,900|
|4400294||Custom TaqMan® Copy Number Assay, SM||720||360|
|4400295||Custom TaqMan® Copy Number Assay, MED||1,500||750|
|4400296||Custom TaqMan® Copy Number Assay, LG||5,800||2,900|
Approximate delivery times are 4-6 days in the US and 6-10 days in Europe.