The assay design could cause later than expected amplification if the assay being used does not detect all of the splice variants for the gene of interest.
|Source of Assays||You need to:|
|TaqMan® Gene Expression Assay||
Check the alignment of the assay with target sequence. When you search TaqMan® assay, there is an Alignment Map link under each assay ID when search result is returned. The link leads to an alignment map as shown below:
|Custom TaqMan® Gene Expression Assay||Submit a template sequence that is common to all of the alternative splicing variants.|
|Assay designed by yourself||Design the primer and probe on a template sequence that is common for all of the alternative splicing variants.|
For Research Use Only. Not for use in diagnostic procedures.