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Ion GeneStudio S5 Systems
Expand your research to cover 2M to 130M sequencing reads per run with this scalable platform
The Ion GeneStudio S5 systems are designed to enable a broad range of targeted next-generation sequencing (NGS) applications with speed and scalability. Five Ion S5 chips enable a sequencing throughput range of 2M to 130M reads per run.
Sequencing and analysis in as little as three hours
As little as 15 minutes hands-on time and same day results.
| Ion GeneStudio S5 System | Ion GeneStudio S5 Plus System | Ion GeneStudio S5 Prime System |
Max. throughput/day (chip type) | 15 Gb | 30 Gb | 50 Gb |
Total sequencing and analysis time at max. throughput | 19 hr (Ion 540 Chip) | 10 hr (Ion 540 Chip) | 6.5 hr (Ion 540 Chip) |
Compatible chips | Ion 510, 520, 530, 540 chips | Ion 510, 520, 530, 540, and 550 chips | |
Sequencing throughput to support multiple applications of NGS
From gene panels to exomes, gene expression profiling to transcriptomes, or microbial genomes to microbiomes, Ion Torrent semiconductor sequencing optimizes cost and throughput for small and large NGS projects.
Cancer research: |  | Inherited disease research: panels for targeted gene or whole exome analysis |
Gene expression analysis: | Reproductive health research: aneuploidy detection, carrier screening research | |
| Microbiology/infectious disease research: microbial whole genomes, microbial typing, and metagenomics |
Cancer research
Enabling NGS analysis and discovery of multi-biomarker types (fusions, insertion/deletions (indels), single nucleotide variants, and copy number variations), Oncomine assays are part of an end-to-end workflow that includes simple, scalable sequencing with optimized bioinformatics and reporting—designed for cancer research.
Logistic regression scores for responders and non-responders
ROC for prediction of response by TCR convergence and evenness
Figure 1. Prediction of immunotherapy outcome via TCR evenness and convergence
Peripheral blood TCRB repertoire convergence and clonal expansion predict response to anti-CTLA-4 monotherapy for cancer
Approach: Leverage the low substitution error rate of the Ion Torrent platform to evaluate convergence as a predictive biomarker for response to anti-CTLA-4 monotherapy in a set of 22 individuals with cancer. Eight peripheral blood leukocyte (PBL) samples derived from three donors were analyzed using the Oncomine TCRB-LR assay or Sequenta/Adaptive Biotechnologies TCRB assay.
Results: The combination of TCR evenness and convergence improves prediction of response (AUC = .89).
Complex and inherited disease research
Our complete NGS solutions are uniquely suited to research in understanding how the combination of genetics and the environment influence development of complex diseases such as autoimmune disorders, neurodegenerative diseases, and many others.
Molecular analysis of inherited cardiomyopathy using next generation semiconductor sequencing technologies
Approach: identify causative variants of different inherited cardiomyopathies using a single Ion AmpliSeq gene panel.
Results: Potential pathogenic variants found in 23.6% of study samples including a homozygous variant in the SLC25A4 gene.
Video: Targeted panels or exome—which is the right NGS approach for inherited disease research?
Microbiology/infectious disease research
Uncover microbial diversity, study pathogen outbreaks, and identify mutations that may be associated with antibiotic resistance. Take advantage of increased throughput, higher accuracy, and longer reads to produce rapid and accurate sequencing of microbes with streamlined sample preparation and a simple, scalable, and optimized data analysis workflow.
A time travel story: metagenomic analyses decipher the unknown geographical shift and the storage history of possibly smuggled antique marble statues
Approach: reconstruct the history of the storage of three single statues to infer the relationship among them, and to elucidate their geographical shift using metagenomic analyses of the 16S ribosomal DNA (rDNA) and other analyses
Results: eukaryotic microbiomes showed similarities comprising many soil-inhabiting organisms. Two statues displayed many halotolerant and halophilic bacteria which may indicate a longer stay in arid and semi-arid surroundings as well as marine environments
Ion Torrent targeted next-generation sequencing workflow
- From as little as 1 ng DNA or RNA input material, prep libraries using FFPE, fine needle aspirates, cell-free DNA, and other sample types using Ion AmpliSeq technology
- Automate library prep, template prep, and chip loading with Ion Chef System automation
- 2.5-hr sequencing runs on Ion GeneStudio S5 instruments with <15 minutes of setup time using simple cartridge-loaded reagents
- Transform data to insight with analysis, QC reporting, and variant calling on Connect cloud-based platform or an in-house Ion Reporter Server
- From as little as 1 ng DNA or RNA input material, prep libraries using FFPE, fine needle aspirates, cell-free DNA, and other sample types using Ion AmpliSeq technology
- Automate library prep, template prep, and chip loading with Ion Chef System automation
- 2.5-hr sequencing runs on Ion GeneStudio S5 instruments with <15 minutes of setup time using simple cartridge-loaded reagents
- Transform data to insight with analysis, QC reporting, and variant calling on Connect cloud-based platform or an in-house Ion Reporter Server
Support
- Get expert support from a global network of over 1,300 service and support personnel dedicated to helping you maximize data integrity and instrument investment
Targeted Gene Sequencing Resources
For Research Use Only. Not for use in diagnostic procedures.